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Journal of Veterinary Research
Volume 63 (2019): Issue 4 (December 2019)
Open Access
Early detection of active glomerular lesions in dogs and cats using podocin
Barbara Szczepankiewicz
Barbara Szczepankiewicz
,
Urszula Pasławska
Urszula Pasławska
,
Marcin Nowak
Marcin Nowak
,
Remigiusz Bąchor
Remigiusz Bąchor
,
Agnieszka Czyżewska-Buczyńska
Agnieszka Czyżewska-Buczyńska
,
Robert Pasławski
Robert Pasławski
and
Zbigniew Szewczuk
Zbigniew Szewczuk
| Dec 19, 2019
Journal of Veterinary Research
Volume 63 (2019): Issue 4 (December 2019)
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Article Category:
Review Article
Published Online:
Dec 19, 2019
Page range:
573 - 577
Received:
Jul 29, 2019
Accepted:
Nov 05, 2019
DOI:
https://doi.org/10.2478/jvetres-2019-0062
Keywords
podocytes
,
podocyturia
,
urinalysis
,
glomerulopathy
,
kidneys
,
markers
© 2019 B. Szczepankiewicz et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Fig. 1
A histopathological assessment of the kidney of a dog without blood azotaemia, suffering from cardiac insufficiency due to severe stage Da mitral endocardiosis according to the ACVIM classification (9) a – H-E stained section. Interstitial, lymphocytic inflammatory infiltration (black arrow) and glomerular hyalinosis and atrophy (white arrow) are visible; b – the immunohistochemical reaction in the kidney section of the same dog, using polyclonal rabbit anti-nephrin antibody (cat no. abx019258, Abexxa, UK, 1:200 dilution). A weak reaction indicates podocyte damage; c – a weak immunohistochemical reaction indicating podocyte damage, using polyclonal rabbit anti-podocin antibody (cat no. abx019257, Abexxa, 1:400 dilution). The sections were washed with Dako Wash Buffer (10×) (product code S3006, Agilent, USA). The Peroxidase/DAB+ Rabbit/Mouse Dako Real EnVision Detection System was used to visualise the immunohistochemical reaction (product code K8006, Agilent). The microphotographs of the studied sections come from our own research and were subjected to computer-aided image analysis using cellSens software (Olympus Soft Imaging Solution GmbH, Germany) coupled with an optical Olympus BX53 microscope equipped with a ColorView IIIu digital camera (Olympus, Japan)
Fig. 2
Multiple reaction monitoring (MRM) chromatograms for the analysis of an H-AAEILAATPAAVQLR-OH peptide, which is a fragment of canine podocin in urine sediment. The retention time of 3.78 min corresponds to the studied compound. The presence of an additional signal at 3.32 min is associated with the presence of cis-trans isomerism of the proline residues (29)