Open Access

Screening of early diagnostic markers of gentamicin-induced acute kidney injury in canines

Jia-San Zheng
Jia-San Zheng
, 
Jing-Nie
Jing-Nie
, 
Ting-Ting Zhu
Ting-Ting Zhu
, 
Hong-Ri Ruan
Hong-Ri Ruan
, 
Xue-Wei
Xue-Wei
 and 
Rui-Wu
Rui-Wu
   | Sep 13, 2019
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Published Online: Sep 13, 2019
Page range: 405 - 411
Received: Feb 07, 2019
Accepted: Jul 24, 2019
DOI: https://doi.org/10.2478/jvetres-2019-0048
Keywords
© 2019 J.-S. Zheng et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Pathological changes in canine kidneys before and after gentamicin administration (HE, 20 ×); a – control kidney tissue; b – appearance of the kidney tissue at the earliest SCr elevation; c – appearance of the kidney tissue after acute kidney injury was established
Pathological changes in canine kidneys before and after gentamicin administration (HE, 20 ×); a – control kidney tissue; b – appearance of the kidney tissue at the earliest SCr elevation; c – appearance of the kidney tissue after acute kidney injury was established

Fig. 2

Ultrastructural changes in canine kidney tissues before and after gentamicin administration (1,700 ×) d – control kidney tissue; e – appearance of the kidney tissue at the earliest SCr elevation; f – appearance of the kidney tissue after acute kidney injury was established
Ultrastructural changes in canine kidney tissues before and after gentamicin administration (1,700 ×) d – control kidney tissue; e – appearance of the kidney tissue at the earliest SCr elevation; f – appearance of the kidney tissue after acute kidney injury was established

Fig. 3

Area under the ROC curve for NGAL and Kim-1
Area under the ROC curve for NGAL and Kim-1

Cutoff concentrations, sensitivity, specificity, and area under the ROC curve of NGAL and Kim-1

Variables Area under the curve (AUC) 95% confidence interval Standard error
NGAL 0.84 0.793–0.887 0.031
Kim-1 0.954 0.941–0.977 0.028

Changes in plasma concentrations of renal injury markers before and after gentamicin administration

Time (h) NGAL content (ng/mL) Kim-1 content (pg/mL) L-FABP content (pg/mL) SCr content (μmol/L)
0 16.42 ± 2.55 29.35 ± 2.82 161.70 ± 9.77 72.50 ± 9.10
6 16.70 ± 2.83 27.97 ± 2.15 151.85 ± 18.24 71.29 ± 6.47
12 18.32 ± 2.27 29.51 ± 4.20 203.78 ± 43.31* 71.79 ± 5.96
18 21.81 ± 2.86*** 33.24 ± 5.60 204.86 ± 44.98* 73.79 ± 5.49
24 24.43 ± 4.02*** 31.11 ± 6.27 197.70 ± 55.87* 71.07 ± 8.47
30 22.39 ± 3.88*** 33.44 ± 6.47* 158.91 ± 38.40 75.79 ± 6.17
36 22.11 ± 3.79*** 39.04 ± 5.86*** 191.15 ± 51.00 73.36 ± 4.03
42 22.13 ± 3.64*** 51.64 ± 7.99*** 192.49 ± 52.17 74.50 ± 11.80
50 22.89 ± 6.19*** 57.95 ± 3.32*** 179.70 ± 18.36 78.36 ± 8.08
56 20.85 ± 3.26** 58.14 ± 4.56*** 195.61 ± 60.94* 74.93 ± 7.99
62 23.63 ± 3.17*** 58.38 ± 5.07*** 168.15 ± 42.62 76.50 ± 6.42
68 22.36 ± 2.37*** 58.99 ± 6.45*** 163.16 ± 33.00 75.29 ± 6.37
72 25.80 ± 2.69*** 59.20 ± 6.22*** 130.70 ± 33.27* 78.50 ± 5.95
78 30.41 ± 4.60*** 58.96 ± 5.46*** 148.56 ± 37.39 82.00 ± 4.41*
84 32.88 ± 3.63*** 67.49 ± 8.58*** 161.701 ± 9.77 87.00 ± 4.63*
90 33.36 ± 7.26*** 69.81 ± 8.73*** 151.85 ± 18.24 84.92 ± 8.84*
96 33.56 ± 7.80*** 85.95 ± 9.20*** 203.78 ± 43.31* 93.75 ± 8.31***
102 37.89 ± 10.99*** 90.51 ± 10.84*** 204.86 ± 44.98* 115.08 ± 16.75***
108 36.74 ± 3.39*** 113.89 ± 28.50*** 197.70 ± 55.87* 129.00 ± 19.42***

Tubular injury score (n = 2)

Group Cortical area (score) Medulla area (score)
Normal control 0.20 ± 0.41 0.25 ± 0.44
Earliest SCr elevation 1.50 ± 0.51 0.70 ± 0.46
Day of acute kidney injury establishment 4.95 ± 0.22 0.73 ± 0.45
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