<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec id="j_jvetres-2019-0041_s_005">
<h3>Introduction</h3>
<p>Comparative analysis of the karyotype structure was made in two hedgehog species: the northern white-breasted hedgehog (<italic>Erinaceus roumanicus)</italic> and the African pygmy hedgehog (<italic>Atelerix albiventris)</italic>.</p></sec>
<sec id="j_jvetres-2019-0041_s_006">
<h3>Material and Methods</h3>
<p>The cytogenetic analysis used differential staining techniques (DAPI, Ag-NOR, and C-banding/DAPI) and sequential QFQ/FISH banding with NOR20 and TEL20 probes which showed 45S rDNA and (TTAGGG)<sub>n</sub> repeat sequences, respectively, on hedgehog chromosomes.</p></sec>
<sec id="j_jvetres-2019-0041_s_007">
<h3>Results</h3>
<p>It was confirmed that the somatic cells of the hedgehogs have a constant number of chromosomes (2n = 48,XY). Differences were observed in the NOR number between the species. NORs were identified on three autosome pairs in the northern white-breasted hedgehog and on only two pairs in the African pygmy hedgehog. Chromosome analysis by C-banding/DAPI showed large segments of heterochromatin rich in A-T pairs on three autosome pairs in both the northern white-breasted and African pygmy hedgehogs. The heterochromatin segments encompassed large fragments of the longer arm of chromosome pairs 13, 14 and 20. The (TTAGGG)<sub>n</sub> repeat sequences on the hedgehog chromosomes were only observed in the terminal position of all the chromosomes in both species.</p></sec>
<sec id="j_jvetres-2019-0041_s_008">
<h3>Conclusion</h3>
<p>Our observations provide new information on the level of diversity within the <italic>Erinaceidae</italic> family.</p></sec>

</abstract>

Introduction
Comparative analysis of the karyotype structure was made in two hedgehog species: the northern white-breasted hedgehog (Erinaceus roumanicus) and the African pygmy hedgehog (Atelerix albiventris).

Material and Methods
The cytogenetic analysis used differential staining techniques (DAPI, Ag-NOR, and C-banding/DAPI) and sequential QFQ/FISH banding with NOR20 and TEL20 probes which showed 45S rDNA and (TTAGGG)n repeat sequences, respectively, on hedgehog chromosomes.

Results
It was confirmed that the somatic cells of the hedgehogs have a constant number of chromosomes (2n = 48,XY). Differences were observed in the NOR number between the species. NORs were identified on three autosome pairs in the northern white-breasted hedgehog and on only two pairs in the African pygmy hedgehog. Chromosome analysis by C-banding/DAPI showed large segments of heterochromatin rich in A-T pairs on three autosome pairs in both the northern white-breasted and African pygmy hedgehogs. The heterochromatin segments encompassed large fragments of the longer arm of chromosome pairs 13, 14 and 20. The (TTAGGG)n repeat sequences on the hedgehog chromosomes were only observed in the terminal position of all the chromosomes in both species.

Conclusion
Our observations provide new information on the level of diversity within the Erinaceidae family.

Cytogenetic karyotype analysis in selected species of the <italic>Erinaceidae</italic> family

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Cytogenetic karyotype analysis in selected species of the  family

Introduction
Comparative analysis of the karyotype structure was made in two hedgehog species: the northern white-breasted hedgehog (Erinaceus roumanicus) and...

Cytogenetic karyotype analysis in selected species of the Erinaceidae family

Published Online: Sep 13, 2019

Page range: 353 - 358

Received: Dec 21, 2019

Accepted: Jul 02, 2019

DOI: https://doi.org/10.2478/jvetres-2019-0041

Keywords
hedgehog, chromosomes, heterochromatin, nucleolar organisers, telomere

© 2019 A. Grzesiakowska et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Cytogenetic karyotype analysis in selected species of the Erinaceidae family

Published Online: Sep 13, 2019

Page range: 353 - 358

Received: Dec 21, 2019

Accepted: Jul 02, 2019

DOI: https://doi.org/10.2478/jvetres-2019-0041

Keywordshedgehog, chromosomes, heterochromatin, nucleolar organisers, telomere

© 2019 A. Grzesiakowska et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Keywords
hedgehog, chromosomes, heterochromatin, nucleolar organisers, telomere