<abstract xmlns="http://www.w3.org/1999/xhtml"><p><bold>Introduction:</bold> Clinical doses of anaesthetic agents were administered to rabbits and effects on the brain, heart, and liver were investigated biochemically and histopathologically. <bold>Material and Methods:</bold> The rabbits were randomly divided into three main groups (16 rabbits each) and each group into study (n = 8) and control (n = 8) groups. All study group rabbits received 3 mg/kg of midazolam (M) intramuscularly. Group 1.1 (M) received nothing further, group 2.1 (MK) also received 25 mg/kg of ketamine, and group 3.1 (MKI) besides ketamine was also given 2% isoflurane to induce anaesthesia for 30 min. NaCl solution in the same volume as midazolam and ketamine was injected into the controls. <bold>Results:</bold> In clinical evaluation significant differences were detected in respiratory and heart rates. In blood gas analysis the PO2 and PCO2 values showed statistical differences in anaesthesia intervals. Significant biochemical value changes were recorded in creatine kinase-Mb, glucose, and total protein. Histopathological liver examinations revealed higher total apoptotic and normal cell numbers in the MK than in the M and MKI groups. Apoptotic cell numbers were statistically significant in M and MK groups. <bold>Conclusion:</bold> Anaesthetic agents may increase programmed apoptosis. The MKI anaesthetics combination was found to cause less cell destruction in general than the other study groups. It was indicated that MKI was the safer anaesthetic combination in rabbits.</p></abstract>

Introduction: Clinical doses of anaesthetic agents were administered to rabbits and effects on the brain, heart, and liver were investigated biochemically and histopathologically. Material and Methods: The rabbits were randomly divided into three main groups (16 rabbits each) and each group into study (n = 8) and control (n = 8) groups. All study group rabbits received 3 mg/kg of midazolam (M) intramuscularly. Group 1.1 (M) received nothing further, group 2.1 (MK) also received 25 mg/kg of ketamine, and group 3.1 (MKI) besides ketamine was also given 2% isoflurane to induce anaesthesia for 30 min. NaCl solution in the same volume as midazolam and ketamine was injected into the controls. Results: In clinical evaluation significant differences were detected in respiratory and heart rates. In blood gas analysis the PO2 and PCO2 values showed statistical differences in anaesthesia intervals. Significant biochemical value changes were recorded in creatine kinase-Mb, glucose, and total protein. Histopathological liver examinations revealed higher total apoptotic and normal cell numbers in the MK than in the M and MKI groups. Apoptotic cell numbers were statistically significant in M and MK groups. Conclusion: Anaesthetic agents may increase programmed apoptosis. The MKI anaesthetics combination was found to cause less cell destruction in general than the other study groups. It was indicated that MKI was the safer anaesthetic combination in rabbits.

Comparison of systemic effects of midazolam, ketamine, and isoflurane anaesthesia in rabbits

Department of Surgery, Veterinary Faculty

Department of Pathology, Veterinary Faculty

Department of Internal Medicine, Veterinary Faculty

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction: Clinical doses of anaesthetic agents were administered to rabbits and effects on the brain, heart, and liver were investigated biochemically and...

Comparison of systemic effects of midazolam, ketamine, and isoflurane anaesthesia in rabbits

Published Online: Jun 20, 2019

Page range: 275 - 283

Received: Dec 18, 2018

Accepted: Apr 16, 2019

DOI: https://doi.org/10.2478/jvetres-2019-0035

Keywords
isoflurane, ketamine, midazolam, systemic effects, histopathology

© 2019 Güneri Atalan et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Comparison of systemic effects of midazolam, ketamine, and isoflurane anaesthesia in rabbits

Published Online: Jun 20, 2019

Page range: 275 - 283

Received: Dec 18, 2018

Accepted: Apr 16, 2019

DOI: https://doi.org/10.2478/jvetres-2019-0035

Keywordsisoflurane, ketamine, midazolam, systemic effects, histopathology

© 2019 Güneri Atalan et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
isoflurane, ketamine, midazolam, systemic effects, histopathology