<abstract xmlns="http://www.w3.org/1999/xhtml"><p><bold>Introduction:</bold> Significant improvement of sperm motility within one month effected by oral supplementation of selenium and vitamin E was described in four infertile male dogs which failed to conceive in their last three matings with different bitches. <bold>Material and Methods:</bold> The dogs (a Golden Retriever, an English Cocker Spaniel, and two Tibetan Mastiffs) were supplemented daily with selenium (Se) (0.6 mg/kg organic Se yeast) and vitamin E (vit. E) (5 mg/kg) <italic>per os</italic> for 60 days. Semen was collected on days 0, 30, 60, and 90. The sperm concentration and motility parameters were evaluated by the CASA system, sperm morphology was explored by Diff-Quick staining, and live and dead spermatozoa were differentiated by eosin/nigrosin staining. The concentrations of Se and vit. E were measured in peripheral blood serum on semen collection days. <bold>Results:</bold> Before administration, the concentrations of Se in blood plasma were low (86.0–165.0 µg/L). After 30 days of treatment there was an observable improvement in total and progressive sperm motility and kinematic parameters (VAP, VSK, VCL, ALH, BCF, and RAPID). The percentages of live and normal morphology sperm cells were also higher. There was also an observable increase in Se and vitamin E concentrations in blood serum. Bitches were successfully mated and delivered four to six puppies. <bold>Conclusion:</bold> Supplementation with Se and vit. E improved rapid sperm motility and restored fertility in infertile dogs with low Se status.</p></abstract>

Introduction: Significant improvement of sperm motility within one month effected by oral supplementation of selenium and vitamin E was described in four infertile male dogs which failed to conceive in their last three matings with different bitches. Material and Methods: The dogs (a Golden Retriever, an English Cocker Spaniel, and two Tibetan Mastiffs) were supplemented daily with selenium (Se) (0.6 mg/kg organic Se yeast) and vitamin E (vit. E) (5 mg/kg) per os for 60 days. Semen was collected on days 0, 30, 60, and 90. The sperm concentration and motility parameters were evaluated by the CASA system, sperm morphology was explored by Diff-Quick staining, and live and dead spermatozoa were differentiated by eosin/nigrosin staining. The concentrations of Se and vit. E were measured in peripheral blood serum on semen collection days. Results: Before administration, the concentrations of Se in blood plasma were low (86.0–165.0 µg/L). After 30 days of treatment there was an observable improvement in total and progressive sperm motility and kinematic parameters (VAP, VSK, VCL, ALH, BCF, and RAPID). The percentages of live and normal morphology sperm cells were also higher. There was also an observable increase in Se and vitamin E concentrations in blood serum. Bitches were successfully mated and delivered four to six puppies. Conclusion: Supplementation with Se and vit. E improved rapid sperm motility and restored fertility in infertile dogs with low Se status.

Improvement of sperm motility within one month under selenium and vitamin E supplementation in four infertile dogs with low selenium status

Department of Animal Reproduction with Clinic, Faculty of Veterinary Medicine

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction: Significant improvement of sperm motility within one month effected by oral supplementation of selenium and vitamin E was described in four...

Improvement of sperm motility within one month under selenium and vitamin E supplementation in four infertile dogs with low selenium status

Published Online: Jun 20, 2019

Page range: 293 - 297

Received: Jan 10, 2019

Accepted: Apr 29, 2019

DOI: https://doi.org/10.2478/jvetres-2019-0025

Keywords
dog, selenium, vitamin E, semen, fertility

© 2019 Anna Domosławska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Improvement of sperm motility within one month under selenium and vitamin E supplementation in four infertile dogs with low selenium status

Published Online: Jun 20, 2019

Page range: 293 - 297

Received: Jan 10, 2019

Accepted: Apr 29, 2019

DOI: https://doi.org/10.2478/jvetres-2019-0025

Keywordsdog, selenium, vitamin E, semen, fertility

© 2019 Anna Domosławska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
dog, selenium, vitamin E, semen, fertility