<abstract xmlns="http://www.w3.org/1999/xhtml"><p><bold>Introduction:</bold> Bovine parainfluenza virus-3 (BPIV3) and bovine respiratory syncytial virus (BRSV) are the cause of respiratory disease in cattle worldwide. With other pathogens, they cause bovine respiratory disease complex (BRDC) in ruminants. The aim of the study was the detection and molecular characterisation of BPIV3 and BRSV from nasal swabs and lung samples of cows in and around the Erzurum region of eastern Turkey. <bold>Material and Methods:</bold> In total, 155 samples were collected. Of animals used in the study 92 were males and 63 females. The age of the animals was between 9 months and 5 years, mean 1.4 years. Most males were in the fattening period and being raised in open sheds; females were in the lactating period and kept in free stall barns. All samples were tested for the presence of viral genes using RT-PCR. Gene-specific primers in a molecular method (RT-PCR) identified BRSV (fusion gene) and BPIV3 (matrix gene) strains at the genus level. <bold>Results:</bold> RNA from BRSV and BPIV3 was detected in two (1.29%) and three (1.93%) samples, respectively, one of each of which was sequenced and the sequences were aligned with reference virus strains. Phylogenetic analyses clustered the strains in genotype C/BPIV3 and subgroup III/BRSV. <bold>Conclusion:</bold> The results indicate that BRSV and BPIV3 contribute to bovine respiratory disease cases in Turkey. This is the first report on their detection and molecular characterisation in ruminants in Turkey.</p></abstract>

Introduction: Bovine parainfluenza virus-3 (BPIV3) and bovine respiratory syncytial virus (BRSV) are the cause of respiratory disease in cattle worldwide. With other pathogens, they cause bovine respiratory disease complex (BRDC) in ruminants. The aim of the study was the detection and molecular characterisation of BPIV3 and BRSV from nasal swabs and lung samples of cows in and around the Erzurum region of eastern Turkey. Material and Methods: In total, 155 samples were collected. Of animals used in the study 92 were males and 63 females. The age of the animals was between 9 months and 5 years, mean 1.4 years. Most males were in the fattening period and being raised in open sheds; females were in the lactating period and kept in free stall barns. All samples were tested for the presence of viral genes using RT-PCR. Gene-specific primers in a molecular method (RT-PCR) identified BRSV (fusion gene) and BPIV3 (matrix gene) strains at the genus level. Results: RNA from BRSV and BPIV3 was detected in two (1.29%) and three (1.93%) samples, respectively, one of each of which was sequenced and the sequences were aligned with reference virus strains. Phylogenetic analyses clustered the strains in genotype C/BPIV3 and subgroup III/BRSV. Conclusion: The results indicate that BRSV and BPIV3 contribute to bovine respiratory disease cases in Turkey. This is the first report on their detection and molecular characterisation in ruminants in Turkey.

Identification and molecular characterisation of bovine parainfluenza virus-3 and bovine respiratory syncytial virus - first report from Turkey

Virology Department, Faculty of Veterinary Medicine

Virology Department, Pendik Veterinary Control Institute

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction: Bovine parainfluenza virus-3 (BPIV3) and bovine respiratory syncytial virus (BRSV) are the cause of respiratory disease in cattle worldwide. With...

Identification and molecular characterisation of bovine parainfluenza virus-3 and bovine respiratory syncytial virus - first report from Turkey

Published Online: Jun 20, 2019

Page range: 167 - 173

Received: Nov 20, 2018

Accepted: Mar 25, 2019

DOI: https://doi.org/10.2478/jvetres-2019-0022

Keywords
cattle, bovine parainfluenza virus-3, bovine respiratory syncytial virus, molecular characterisation, Turkey

© 2019 Mehmet Ozkan Timurkan et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Identification and molecular characterisation of bovine parainfluenza virus-3 and bovine respiratory syncytial virus - first report from Turkey

Published Online: Jun 20, 2019

Page range: 167 - 173

Received: Nov 20, 2018

Accepted: Mar 25, 2019

DOI: https://doi.org/10.2478/jvetres-2019-0022

Keywordscattle, bovine parainfluenza virus-3, bovine respiratory syncytial virus, molecular characterisation, Turkey

© 2019 Mehmet Ozkan Timurkan et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
cattle, bovine parainfluenza virus-3, bovine respiratory syncytial virus, molecular characterisation, Turkey