<abstract xmlns="http://www.w3.org/1999/xhtml"><bold>Introduction:</bold> Quercetin is a polyphenolic flavonoid which has been used in traditional Chinese medicine as a natural therapeutic agent with a broad spectrum of activities (antioxidant, anticancer, neuroprotective, anti-inflammatory, antiviral and antibacterial). The aim of this study was to develop and validate a rapid and simple ultra-high-performance liquid chromatography with tandem mass spectrometry (UHPLC-MS/MS) method for the determination of quercetin in milk.<bold>Material and Methods:</bold> Sample preparation was based on a liquid-liquid extraction with 0.5% formic acid in acetonitrile. The chromatographic separation was performed on a ZORBAX SB-C18 column with methanol and 0.5% formic acid as a mobile phase.<bold>Results:</bold> The procedure was successfully validated. The mean recovery of the analyte was 98%, with the corresponding intra- and inter-day variation less than 10% and 15%, respectively, and the repeatability and reproducibility were in the range of 3%–7.2% and 6.1%–12%, respectively. The lowest level of quantification was 1.0 μg/kg.<bold>Conclusion:</bold> The proposed method was successfully applied in evaluating the pharmacokinetics of quercetin in milk obtained from dairy cows with clinical mastitis after intramammary administration.</abstract>

Introduction: Quercetin is a polyphenolic flavonoid which has been used in traditional Chinese medicine as a natural therapeutic agent with a broad spectrum of activities (antioxidant, anticancer, neuroprotective, anti-inflammatory, antiviral and antibacterial). The aim of this study was to develop and validate a rapid and simple ultra-high-performance liquid chromatography with tandem mass spectrometry (UHPLC-MS/MS) method for the determination of quercetin in milk.Material and Methods: Sample preparation was based on a liquid-liquid extraction with 0.5% formic acid in acetonitrile. The chromatographic separation was performed on a ZORBAX SB-C18 column with methanol and 0.5% formic acid as a mobile phase.Results: The procedure was successfully validated. The mean recovery of the analyte was 98%, with the corresponding intra- and inter-day variation less than 10% and 15%, respectively, and the repeatability and reproducibility were in the range of 3%–7.2% and 6.1%–12%, respectively. The lowest level of quantification was 1.0 μg/kg.Conclusion: The proposed method was successfully applied in evaluating the pharmacokinetics of quercetin in milk obtained from dairy cows with clinical mastitis after intramammary administration.

Development of a UHPLC-MS/MS method for the determination of quercetin in milk and its application to a pharmacokinetic study

Department of Pharmacology and Toxicology

Department of Pharmacology, Faculty of Veterinary Medicine

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction: Quercetin is a polyphenolic flavonoid which has been used in traditional Chinese medicine as a natural therapeutic agent with a broad spectrum of...

Development of a UHPLC-MS/MS method for the determination of quercetin in milk and its application to a pharmacokinetic study

Published Online: Mar 28, 2019

Page range: 87 - 91

Received: Sep 04, 2018

Accepted: Feb 27, 2019

DOI: https://doi.org/10.2478/jvetres-2019-0013

Keywords
quercetin, UHPLC-MS/MS, milk, pharmacokinetics

© 2019 Małgorzata Gbylik-Sikorska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Development of a UHPLC-MS/MS method for the determination of quercetin in milk and its application to a pharmacokinetic study

Published Online: Mar 28, 2019

Page range: 87 - 91

Received: Sep 04, 2018

Accepted: Feb 27, 2019

DOI: https://doi.org/10.2478/jvetres-2019-0013

Keywordsquercetin, UHPLC-MS/MS, milk, pharmacokinetics

© 2019 Małgorzata Gbylik-Sikorska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
quercetin, UHPLC-MS/MS, milk, pharmacokinetics