<abstract xmlns="http://www.w3.org/1999/xhtml"><bold>Introduction:</bold> In calves, hyposelenosis degenerates skeletal muscles in different parts of the body. The extent of damage to muscle cells can be diagnosed by determining the activity of creatine kinase (CK), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH). The aim of this study was to analyse variations in the serum levels of LDH isoenzymes in calves with nutritional muscular dystrophy (NMD), to determine the applicability of this parameter for diagnosing NMD, and to describe the influence of hyposelenosis on total protein (TP), triglyceride (TG), and cholesterol (CHOL) levels.<bold>Material and Methods:</bold> Two groups of calves (n = six animals per group) were used. After birth, control group calves (SC) were intramuscularly administered 10 ml of a preparation containing selenium (Se) and vitamin E, and experimental group animals (SE) that were not injected. Blood was collected after 5, 15, and 25 days, and the concentrations of Se, vitamin E, TP, TG, and CHOL and the activity of glutathione peroxidase (GSH-Px), CK, and LDH fractions were determined.<bold>Results:</bold> Hypocholesterolaemia and elevated TG levels were found in SE group calves whose LDH fractions revealed a significant increase in LDH4 and LDH5 activity and a decrease in LDH1 activity when electrophoretically separated.<bold>Conclusions:</bold> Nutritional muscular dystrophy is accompanied by hypocholesterolaemia and elevated TG levels caused by muscle lipolysis. LDH4 and LDH5 activity parameters assist early diagnosis of NMD in calves.</abstract>

Introduction: In calves, hyposelenosis degenerates skeletal muscles in different parts of the body. The extent of damage to muscle cells can be diagnosed by determining the activity of creatine kinase (CK), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH). The aim of this study was to analyse variations in the serum levels of LDH isoenzymes in calves with nutritional muscular dystrophy (NMD), to determine the applicability of this parameter for diagnosing NMD, and to describe the influence of hyposelenosis on total protein (TP), triglyceride (TG), and cholesterol (CHOL) levels.Material and Methods: Two groups of calves (n = six animals per group) were used. After birth, control group calves (SC) were intramuscularly administered 10 ml of a preparation containing selenium (Se) and vitamin E, and experimental group animals (SE) that were not injected. Blood was collected after 5, 15, and 25 days, and the concentrations of Se, vitamin E, TP, TG, and CHOL and the activity of glutathione peroxidase (GSH-Px), CK, and LDH fractions were determined.Results: Hypocholesterolaemia and elevated TG levels were found in SE group calves whose LDH fractions revealed a significant increase in LDH4 and LDH5 activity and a decrease in LDH1 activity when electrophoretically separated.Conclusions: Nutritional muscular dystrophy is accompanied by hypocholesterolaemia and elevated TG levels caused by muscle lipolysis. LDH4 and LDH5 activity parameters assist early diagnosis of NMD in calves.

Applicability of the protein-lipid profile and activity of lactate dehydrogenase isoenzymes for diagnosing nutritional muscular dystrophy in calves

Department and Clinic of Internal Diseases, Faculty of Veterinary Medicine

Department of Animal Reproduction with Clinic, Faculty of Veterinary Medicine

Division of Functional Genomics in Biological and Biomedical Science, Interdisciplinary Centre of Modern Technology

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction: In calves, hyposelenosis degenerates skeletal muscles in different parts of the body. The extent of damage to muscle cells can be diagnosed by...

Applicability of the protein-lipid profile and activity of lactate dehydrogenase isoenzymes for diagnosing nutritional muscular dystrophy in calves

Published Online: Dec 31, 2018

Page range: 503 - 509

Received: Jul 26, 2018

Accepted: Nov 30, 2018

DOI: https://doi.org/10.2478/jvetres-2018-0072

Keywords
calf, selenium, nutritional muscular dystrophy, isoenzymes

© 2018 Katarzyna Żarczyńska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Applicability of the protein-lipid profile and activity of lactate dehydrogenase isoenzymes for diagnosing nutritional muscular dystrophy in calves

Published Online: Dec 31, 2018

Page range: 503 - 509

Received: Jul 26, 2018

Accepted: Nov 30, 2018

DOI: https://doi.org/10.2478/jvetres-2018-0072

Keywordscalf, selenium, nutritional muscular dystrophy, isoenzymes

© 2018 Katarzyna Żarczyńska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
calf, selenium, nutritional muscular dystrophy, isoenzymes