<abstract xmlns="http://www.w3.org/1999/xhtml"><bold>Introduction:</bold> Iron and molybdenum are essential trace elements for cell metabolism. They are involved in maintaining proper functions of enzymes, cell proliferation, and metabolism of DNA.<bold>Material and Methods:</bold> BALB/3T3 and HepG2 cells were incubated with iron chloride or molybdenum trioxide at concentrations from 100 to 1,400 µM. The cells were also incubated in mixtures of iron chloride at 200 μM plus molybdenum trioxide at 1,000 μM or iron chloride at 1,000 μM plus molybdenum trioxide at 200 μM. Cell viability was determined with MTT reduction, LHD release, and NRU tests.<bold>Results:</bold> A decrease in cell viability was observed after incubating both cell lines with iron chloride or molybdenum trioxide. In cells incubated with mixtures of these trace elements, a decrease in cell viability was observed, assessed by all the used assays.<bold>Conclusions:</bold> Iron (III) and molybdenum (III) decrease cell viability in normal and cancer cells. A synergistic effect of the mixture of these elements was observed.</abstract>

Introduction: Iron and molybdenum are essential trace elements for cell metabolism. They are involved in maintaining proper functions of enzymes, cell proliferation, and metabolism of DNA.Material and Methods: BALB/3T3 and HepG2 cells were incubated with iron chloride or molybdenum trioxide at concentrations from 100 to 1,400 µM. The cells were also incubated in mixtures of iron chloride at 200 μM plus molybdenum trioxide at 1,000 μM or iron chloride at 1,000 μM plus molybdenum trioxide at 200 μM. Cell viability was determined with MTT reduction, LHD release, and NRU tests.Results: A decrease in cell viability was observed after incubating both cell lines with iron chloride or molybdenum trioxide. In cells incubated with mixtures of these trace elements, a decrease in cell viability was observed, assessed by all the used assays.Conclusions: Iron (III) and molybdenum (III) decrease cell viability in normal and cancer cells. A synergistic effect of the mixture of these elements was observed.

Cytotoxicity of iron (III), molybdenum (III), and their mixtures in BALB/3T3 and HepG2 cells

The John Paul II Catholic University of Lublin

Department of Anaesthesiology and Intensive Care, Faculty of Medicine

Department of Microbiology and Clinical Immunology, Faculty of Veterinary Medicine

University of Warmia and Mazury in Olsztyn

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction: Iron and molybdenum are essential trace elements for cell metabolism. They are involved in maintaining proper functions of enzymes, cell...

Cytotoxicity of iron (III), molybdenum (III), and their mixtures in BALB/3T3 and HepG2 cells

Published Online: Dec 31, 2018

Page range: 527 - 533

Received: Jul 12, 2018

Accepted: Nov 19, 2018

DOI: https://doi.org/10.2478/jvetres-2018-0066

Keywords
molybdenum (III), iron (III), cytotoxicity, interactions

© 2018 Sylwia Terpiłowska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Cytotoxicity of iron (III), molybdenum (III), and their mixtures in BALB/3T3 and HepG2 cells

Published Online: Dec 31, 2018

Page range: 527 - 533

Received: Jul 12, 2018

Accepted: Nov 19, 2018

DOI: https://doi.org/10.2478/jvetres-2018-0066

Keywordsmolybdenum (III), iron (III), cytotoxicity, interactions

© 2018 Sylwia Terpiłowska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
molybdenum (III), iron (III), cytotoxicity, interactions