<abstract xmlns="http://www.w3.org/1999/xhtml"><sec id="j_jvetres-2018-0045_s_005_w2aab3b7c12b1b6b1aab1c16b1Aa"><h3>Introduction</h3><p>Epstein–Barr virus (EBV) is a γ-herpesvirus associated with various neoplasms in humans and is a probable aetiological agent in breast cancer; however, a causal relationship has not yet been established. Because of the epidemiological and clinicopathological similarities between breast cancer and canine mammary tumours, dogs have been proposed as a valid model for breast cancer.</p></sec><sec id="j_jvetres-2018-0045_s_006_w2aab3b7c12b1b6b1aab1c16b2Aa"><h3>Material and Methods</h3><p>A total of 47 canine mammary gland tumour tissues were processed by routine histopathological technique with haematoxylin-eosin staining and classified according to the type of neoplasm. DNA was extracted from paraffin-embedded tissues and the EBNA-1 gene and the <italic>Bam</italic>HI-W region specific for EBV were evaluated by nested PCR.</p></sec><sec id="j_jvetres-2018-0045_s_007_w2aab3b7c12b1b6b1aab1c16b3Aa"><h3>Results</h3><p>The histopathological evaluation revealed 2 benign neoplasms, and many carcinomas: 2 <italic>in situ</italic>, 9 simple, 3 solid, 10 complex, and 21 mixed. One sample was positive for the EBNA-1 gene, while all were negative for the <italic>Bam</italic>HI-W region.</p></sec><sec id="j_jvetres-2018-0045_s_008_w2aab3b7c12b1b6b1aab1c16b4Aa"><h3>Conclusion</h3><p>No association was found between EBV and mammary tumours in dogs. However, here we report for the first time the presence of an EBV gene sequence in a canine mammary tumour. It is likely that detection of EBV might be affected by the quality and quantity of DNA extracted from paraffin-embedded tissues. Additional studies are necessary to establish any association of EBV with mammary gland cancer in humans and in dogs, which could eventually lead to better public health prevention and control.</p></sec></abstract>

IntroductionEpstein–Barr virus (EBV) is a γ-herpesvirus associated with various neoplasms in humans and is a probable aetiological agent in breast cancer; however, a causal relationship has not yet been established. Because of the epidemiological and clinicopathological similarities between breast cancer and canine mammary tumours, dogs have been proposed as a valid model for breast cancer.Material and MethodsA total of 47 canine mammary gland tumour tissues were processed by routine histopathological technique with haematoxylin-eosin staining and classified according to the type of neoplasm. DNA was extracted from paraffin-embedded tissues and the EBNA-1 gene and the BamHI-W region specific for EBV were evaluated by nested PCR.ResultsThe histopathological evaluation revealed 2 benign neoplasms, and many carcinomas: 2 in situ, 9 simple, 3 solid, 10 complex, and 21 mixed. One sample was positive for the EBNA-1 gene, while all were negative for the BamHI-W region.ConclusionNo association was found between EBV and mammary tumours in dogs. However, here we report for the first time the presence of an EBV gene sequence in a canine mammary tumour. It is likely that detection of EBV might be affected by the quality and quantity of DNA extracted from paraffin-embedded tissues. Additional studies are necessary to establish any association of EBV with mammary gland cancer in humans and in dogs, which could eventually lead to better public health prevention and control.

Lack of association between Epstein–Barr virus and mammary tumours in dogs

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"Lack of association between Epstein\u2013Barr virus and mammary tumours in dogs"}

IntroductionEpstein–Barr virus (EBV) is a γ-herpesvirus associated with various neoplasms in humans and is a probable aetiological agent in breast cancer;...

Lack of association between Epstein–Barr virus and mammary tumours in dogs

Published Online: Oct 23, 2018

Page range: 309 - 315

Received: Apr 09, 2018

Accepted: Aug 24, 2018

DOI: https://doi.org/10.2478/jvetres-2018-0045

Keywords
dogs, Epstein–Barr virus, mammary gland cancer, comparative pathology

© 2018 G.A.R. López et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Lack of association between Epstein–Barr virus and mammary tumours in dogs

Published Online: Oct 23, 2018

Page range: 309 - 315

Received: Apr 09, 2018

Accepted: Aug 24, 2018

DOI: https://doi.org/10.2478/jvetres-2018-0045

Keywordsdogs, Epstein–Barr virus, mammary gland cancer, comparative pathology

© 2018 G.A.R. López et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
dogs, Epstein–Barr virus, mammary gland cancer, comparative pathology