<abstract xmlns="http://www.w3.org/1999/xhtml"><sec id="j_jvetres-2018-0033_s_005_w2aab3b7c16b1b6b1aab1c16b1Aa"><h3>Introduction</h3><p>Glucagon-like peptide-1 (GLP-1) is a polypeptide that is mainly produced by intestinal L cells and is encoded by the proglucagon gene. In this study, GLP-1 localisation was investigated in the ileum of healthy and diabetic mice by immunohistochemistry and proglucagon gene expression was assayed by reverse transcription-polymerase chain reaction.</p></sec><sec id="j_jvetres-2018-0033_s_006_w2aab3b7c16b1b6b1aab1c16b2Aa"><h3>Material and Methods</h3><p>This study included 18 male <italic>Balb/c</italic> mice that were divided into diabetic, sham, and control groups. Mice in the diabetic group received 100 mg/kg of streptozotocin. Immunohistochemical expression of GLP-1 was determined using the avidin–biotin–peroxidase complex technique, and proglucagon gene expression was determined by RT-PCR.</p></sec><sec id="j_jvetres-2018-0033_s_007_w2aab3b7c16b1b6b1aab1c16b3Aa"><h3>Results</h3><p>Analysis of GLP-1 immunohistochemical localisation showed that GLP-1-immunopositive cells (L cells) were present between epithelial cells in the intestinal crypts. The intensity and localisation of GLP-1 immunoreactivity were similar among the mice in all the groups. Proglucagon gene expression levels were also statistically similar among the mice in all the groups.</p></sec><sec id="j_jvetres-2018-0033_s_008_w2aab3b7c16b1b6b1aab1c16b4Aa"><h3>Conclusion</h3><p>No difference was demonstrated among the mice in the diabetic, sham, or control groups with respect to proglucagon gene expression and GLP-1 localisation in the ileum, suggesting that diabetes does not affect proglucagon gene expression in the ileum.</p></sec></abstract>

IntroductionGlucagon-like peptide-1 (GLP-1) is a polypeptide that is mainly produced by intestinal L cells and is encoded by the proglucagon gene. In this study, GLP-1 localisation was investigated in the ileum of healthy and diabetic mice by immunohistochemistry and proglucagon gene expression was assayed by reverse transcription-polymerase chain reaction.Material and MethodsThis study included 18 male Balb/c mice that were divided into diabetic, sham, and control groups. Mice in the diabetic group received 100 mg/kg of streptozotocin. Immunohistochemical expression of GLP-1 was determined using the avidin–biotin–peroxidase complex technique, and proglucagon gene expression was determined by RT-PCR.ResultsAnalysis of GLP-1 immunohistochemical localisation showed that GLP-1-immunopositive cells (L cells) were present between epithelial cells in the intestinal crypts. The intensity and localisation of GLP-1 immunoreactivity were similar among the mice in all the groups. Proglucagon gene expression levels were also statistically similar among the mice in all the groups.ConclusionNo difference was demonstrated among the mice in the diabetic, sham, or control groups with respect to proglucagon gene expression and GLP-1 localisation in the ileum, suggesting that diabetes does not affect proglucagon gene expression in the ileum.

GLP-1 localisation and proglucagon gene expression in healthy and diabetic mouse ileum

Department of Histology and Embryology, Faculty of Veterinary Medicine

Department of Histology and Embryology, Faculty of Medicine

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

IntroductionGlucagon-like peptide-1 (GLP-1) is a polypeptide that is mainly produced by intestinal L cells and is encoded by the proglucagon gene. In this...

Group	N	Blood glucose (mg/dL) minimum	Blood glucose (mg/dL) maximum	Blood glucose (mg/dL) mean ± SD
Diabetic	6	240	360	275.66^a ± 45.71
Control	6	74	83	79.50^b ± 3.27
Sham	6	72	84	79.33^b ± 4.13

Gene		Primers of genes	Reference number
β-actin 5'		5'-TCATGAAGTGTGACGTTGACATCCGT-3'	20
β-actin 3'		5'-CCTAGAAGCATTTGCGGTGCACGATG-3'	20
Proglucagon	(GCG) 5'	5'-GACTTCCCAGAAGAAGTCGCCAT-3'	28
Proglucagon	(GCG) 3'	5'-CTACGGTTACCAGGTGGTCATGT-3'	28

GLP-1 localisation and proglucagon gene expression in healthy and diabetic mouse ileum

Published Online: Jul 07, 2018

Page range: 237 - 242

Received: Mar 29, 2018

Accepted: Jun 22, 2018

DOI: https://doi.org/10.2478/jvetres-2018-0033

Keywords
mice, diabetes mellitus, GLP-1, L cells, proglucagon

© 2018 Serap Koral Taşçı et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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Blood glucose level (mg/dL) on the 3rd day. Comparison among groups

Sequences of β-actin and proglucagon (GCG) specific primer pairs

GLP-1 localisation and proglucagon gene expression in healthy and diabetic mouse ileum

Published Online: Jul 07, 2018

Page range: 237 - 242

Received: Mar 29, 2018

Accepted: Jun 22, 2018

DOI: https://doi.org/10.2478/jvetres-2018-0033

Keywordsmice, diabetes mellitus, GLP-1, L cells, proglucagon

© 2018 Serap Koral Taşçı et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Fig. 2

Fig. 3

Fig. 4

Fig. 5

Fig. 6

Fig. 7

Fig. 8

Blood glucose level (mg/dL) on the 3rd day. Comparison among groups

Sequences of β-actin and proglucagon (GCG) specific primer pairs

Keywords
mice, diabetes mellitus, GLP-1, L cells, proglucagon