<abstract xmlns="http://www.w3.org/1999/xhtml"><sec id="j_jvetres-2018-0031_s_005_w2aab3b7c14b1b6b1aab1c16b1Aa"><h3>Introduction</h3><p>Inosine pranobex (Isoprinosine) stimulates cell-mediated immune responses to viral infections in humans and might have also therapeutic use in animals. The aim of this study was to compare three <italic>in vitro</italic> cytotoxicity assays on mouse embryo fibroblasts and liver cancer cells and determine their ability to detect early cytotoxic effects for inosine pranobex.</p></sec><sec id="j_jvetres-2018-0031_s_006_w2aab3b7c14b1b6b1aab1c16b2Aa"><h3>Material and Methods</h3><p>BALB/3T3 clone A31and HepG2 cells were incubated with inosine pranobex at concentrations from 0.1 to 1,000 μg/mL. Cell viability was determined with the MTT reduction, the LHD release, and the NRU tests.</p></sec><sec id="j_jvetres-2018-0031_s_007_w2aab3b7c14b1b6b1aab1c16b3Aa"><h3>Results</h3><p>A decrease in the cell viability was observed after incubating the BALB/3T3 clone A31and HepG2 cells with inosine pranobex.</p></sec><sec id="j_jvetres-2018-0031_s_008_w2aab3b7c14b1b6b1aab1c16b4Aa"><h3>Conclusions</h3><p>Based on the cytotoxicity endpoints measured in these investigations in BALB/3T3 clone A31cells, it can be concluded that the cell membrane may be the first part of the cell to be affected by inosine pranobex. The disintegration of lysosomes and mitochondria follows mitochondria damage. In HepG2 cells likewise, the cell membrane may be the first part of the cell to be affected by inosine pranobex. Also in liver cancer cells, the disintegration of mitochondria (assessed with the MTT reduction assay) and next of lysosomes (assessed with the NRU assay) follows mitochondria damage.</p></sec></abstract>

IntroductionInosine pranobex (Isoprinosine) stimulates cell-mediated immune responses to viral infections in humans and might have also therapeutic use in animals. The aim of this study was to compare three in vitro cytotoxicity assays on mouse embryo fibroblasts and liver cancer cells and determine their ability to detect early cytotoxic effects for inosine pranobex.Material and MethodsBALB/3T3 clone A31and HepG2 cells were incubated with inosine pranobex at concentrations from 0.1 to 1,000 μg/mL. Cell viability was determined with the MTT reduction, the LHD release, and the NRU tests.ResultsA decrease in the cell viability was observed after incubating the BALB/3T3 clone A31and HepG2 cells with inosine pranobex.ConclusionsBased on the cytotoxicity endpoints measured in these investigations in BALB/3T3 clone A31cells, it can be concluded that the cell membrane may be the first part of the cell to be affected by inosine pranobex. The disintegration of lysosomes and mitochondria follows mitochondria damage. In HepG2 cells likewise, the cell membrane may be the first part of the cell to be affected by inosine pranobex. Also in liver cancer cells, the disintegration of mitochondria (assessed with the MTT reduction assay) and next of lysosomes (assessed with the NRU assay) follows mitochondria damage.

Influence of inosine pranobex on cell viability in normal fibroblasts and liver cancer cells

The John Paul II Catholic University of Lublin

Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine

University of Warmia and Mazury in Olsztyn

Department of Microbiology and Clinical Immunology, Faculty of Veterinary Medicine

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

IntroductionInosine pranobex (Isoprinosine) stimulates cell-mediated immune responses to viral infections in humans and might have also therapeutic use in...

	BALB/ 3T3 clone A31 line	HepG2 line
MTT reduction assay	500	50
LDH release assay	100	10
NRU assay	500	100

Influence of inosine pranobex on cell viability in normal fibroblasts and liver cancer cells

Published Online: Jul 07, 2018

Page range: 215 - 220

Received: Mar 29, 2018

Accepted: Jun 21, 2018

DOI: https://doi.org/10.2478/jvetres-2018-0031

Keywords
inosine pranobex, cell culture, cytotoxicity

© 2018 Sylwia Tobólska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Fig. 2

Fig. 3

Cytotoxicity of inosine pranobex. Inhibitory concentration (IC50, μg/mL)

Influence of inosine pranobex on cell viability in normal fibroblasts and liver cancer cells

Published Online: Jul 07, 2018

Page range: 215 - 220

Received: Mar 29, 2018

Accepted: Jun 21, 2018

DOI: https://doi.org/10.2478/jvetres-2018-0031

Keywordsinosine pranobex, cell culture, cytotoxicity

© 2018 Sylwia Tobólska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Fig. 2

Fig. 3

Cytotoxicity of inosine pranobex. Inhibitory concentration (IC50, μg/mL)

Keywords
inosine pranobex, cell culture, cytotoxicity