Cloning and differential expression analyses of Cdc42 from sheep
Published Online: Mar 30, 2018
Page range: 113 - 119
Received: Sep 07, 2017
Accepted: Feb 14, 2018
DOI: https://doi.org/10.2478/jvetres-2018-0016
Keywords
© 2018 Y.-J. Yang1et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Introduction
Serological diagnosis of brucellosis is still a great challenge due to the infeasibility of discriminating infected animals from vaccinated ones, so it is necessary to search for diagnostic biomarkers for differential diagnosis of brucellosis.
Material and Methods
Cell division cycle 42 (Cdc42) from sheep (
Results
The full-length cDNA of OaCdc42 was 1,609 bp containing an open reading frame (ORF) of 576 bp. OaCdc42 mRNAs were detected in the heart, liver, spleen, lung, kidneys, rumen, small intestine, skeletal muscles, and buffy coat, and the highest expression was detected in the small intestine. Compared to the control, the levels of OaCdc42 mRNA from sheep infected with
Conclusion
OaCdc42 is not a good potential diagnostic biomarker for differential diagnosis of brucellosis in sheep.