<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec id="j_jvetres-2018-0006_s_005">
<h3>Introduction</h3>
<p>Phagocytic activity and oxygen metabolism of peripheral blood granulocytes from rabbits with experimental trichophytosis were assessed by flow cytometry.</p></sec>
<sec id="j_jvetres-2018-0006_s_006">
<h3>Material and Methods</h3>
<p>Virulent species of <italic>T</italic>. <italic>mentagrophytes</italic> var. <italic>granulosum</italic> (Tm-K) isolated from rabbits with natural trichophytosis was used for experimental infection. The phagocytic activity of granulocytes was measured in whole blood by flow cytometry using the commercial Phagotest kit. Oxidative burst was measured in whole blood by flow cytometry using the commercial Bursttest kit.</p></sec>
<sec id="j_jvetres-2018-0006_s_007">
<h3>Results</h3>
<p>It was found that rabbits were susceptible to infection with <italic>Trichophyton mentagrophytes</italic> under experimental conditions. The analysis of the phagocytic activity indices and oxygen metabolism of granulocytes in peripheral blood of infected rabbits showed that changes of the indices were connected with the progression and regression of the disease. A significant decrease in phagocytic activity and oxygen metabolism was observed during development of fungal lesions and it remained similar throughout the progress of the disease. The highest means of the percentage of activated and ingesting phagocytes and a significant increase in the mean fluorescence intensity (representing the number of ingested bacteria) were observed during spontaneous recovery. Therefore, the decrease or increase in the indices of phagocytic activity and oxygen metabolism of granulocytes from rabbits experimentally infected with <italic>T</italic>. <italic>mentagrophytes</italic> is somehow related to the progress of infection and suppressive activity of the fungus, whose elimination during recovery caused significant increases in investigated indices of non-specific cellular immunity.</p></sec>
<sec id="j_jvetres-2018-0006_s_008">
<h3>Conclusion</h3>
<p>The results of the present investigation confirm that the mechanism of oxygen-dependent killing is crucial in infections caused by <italic>T</italic>. <italic>mentagrophytes</italic>.</p></sec>
</abstract>

Introduction
Phagocytic activity and oxygen metabolism of peripheral blood granulocytes from rabbits with experimental trichophytosis were assessed by flow cytometry.

Material and Methods
Virulent species of T. mentagrophytes var. granulosum (Tm-K) isolated from rabbits with natural trichophytosis was used for experimental infection. The phagocytic activity of granulocytes was measured in whole blood by flow cytometry using the commercial Phagotest kit. Oxidative burst was measured in whole blood by flow cytometry using the commercial Bursttest kit.

Results
It was found that rabbits were susceptible to infection with Trichophyton mentagrophytes under experimental conditions. The analysis of the phagocytic activity indices and oxygen metabolism of granulocytes in peripheral blood of infected rabbits showed that changes of the indices were connected with the progression and regression of the disease. A significant decrease in phagocytic activity and oxygen metabolism was observed during development of fungal lesions and it remained similar throughout the progress of the disease. The highest means of the percentage of activated and ingesting phagocytes and a significant increase in the mean fluorescence intensity (representing the number of ingested bacteria) were observed during spontaneous recovery. Therefore, the decrease or increase in the indices of phagocytic activity and oxygen metabolism of granulocytes from rabbits experimentally infected with T. mentagrophytes is somehow related to the progress of infection and suppressive activity of the fungus, whose elimination during recovery caused significant increases in investigated indices of non-specific cellular immunity.

Conclusion
The results of the present investigation confirm that the mechanism of oxygen-dependent killing is crucial in infections caused by T. mentagrophytes.

Phagocytic activity and oxygen metabolism of peripheral blood granulocytes from rabbits experimentally infected with <italic>Trichophyton mentagrophytes</italic>

Department of Epizootiology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Phagocytic activity and oxygen metabolism of peripheral blood granulocytes from rabbits experimentally infected with 

Introduction
Phagocytic activity and oxygen metabolism of peripheral blood granulocytes from rabbits with experimental trichophytosis were assessed by flow...

Phagocytic activity and oxygen metabolism of peripheral blood granulocytes from rabbits experimentally infected with Trichophyton mentagrophytes

Published Online: Mar 30, 2018

Page range: 43 - 48

Received: May 11, 2017

Accepted: Feb 14, 2018

DOI: https://doi.org/10.2478/jvetres-2018-0006

Keywords
rabbits, phagocytic activity, oxygen metabolism, granulocytes

© 2018 K. Wojcicka-Lorenowicz et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1a

Fig. 1b

Fig. 2a

Fig. 2b

Phagocytic activity and oxygen metabolism of peripheral blood granulocytes from rabbits experimentally infected with Trichophyton mentagrophytes

Published Online: Mar 30, 2018

Page range: 43 - 48

Received: May 11, 2017

Accepted: Feb 14, 2018

DOI: https://doi.org/10.2478/jvetres-2018-0006

Keywordsrabbits, phagocytic activity, oxygen metabolism, granulocytes

© 2018 K. Wojcicka-Lorenowicz et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1a

Fig. 1b

Fig. 2a

Fig. 2b

Keywords
rabbits, phagocytic activity, oxygen metabolism, granulocytes