Maximizing Hybrid Seedlings Recovery and Early Identification of Highly Polyembryonic Acid Lime (Citrus aurantifolia Swing.) × Lemon (Citrus limon Burm.) Hybrids using SSR Markers
, , and | Dec 18, 2020
About this article
Published Online: Dec 18, 2020
Page range: 43 - 52
Received: Jun 01, 2020
Accepted: Nov 01, 2020
DOI: https://doi.org/10.2478/johr-2020-0024
Keywords
© 2020 Anil Kumar Dubey et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Figure 1
Figure 2
Effect of embryo age and media composition on embryo germination, days taken for germination and survival of ‘Kagzi’ (acid lime) × ‘Konkan Seedless’ (lemon) seedlings
| Medium composition | No. of embryo cultured | No. of germinated embryos | Days to initiate germination | Days to complete germination | Germination (%) | Seedling survival at 60 DAI |
|---|---|---|---|---|---|---|
| M1 | 25 | 19 | 3 | 8 | 76 cd | 84 a |
| M2 | 25 | 24 | 3 | 8 | 96 a | 71 cb |
| M3 | 27 | 22 | 3 | 8 | 81 cb | 73 cb |
| M1 | 26 | 16 | 2 | 9 | 62 e | 75 b |
| M2 | 26 | 21 | 2 | 11 | 81 cb | 67 c |
| M3 | 26 | 26 | 2 | 7 | 100 a | 73 cb |
| M1 | 16 | 12 | 2 | 7 | 75 d | 67 c |
| M2 | 15 | 13 | 2 | 7 | 87 b | 69 cb |
| M3 | 15 | 15 | 2 | 7 | 100 a | 53 d |
Effect of embryo age on number of seeds per fruit and seeds with and without embryos in ‘Kagzi’ (acid lime) × ‘Konkan Seedless’ (lemon) crosses
| Embryo age (DAP) | No. of seeds per fruit | No. of seeds obtained | No. of seeds containing functional embryos | Seeds with functional embryos (%) |
|---|---|---|---|---|
| 80–90 | 22 | 88 | 77 | 88 |
| 110–120 | 32 | 96 | 76 | 79 |
| 130–140 | 27 | 53 | 46 | 90 |
Effect of embryo age and media composition on plantlets growth obtained from ‘Kagzi’ (acid lime) × ‘Konkan Seedless’ (lemon) crosses at 30 and 60 DAI
| Medium composition | Root length (mm) | Shoot length (mm) | Leaves per plantlet | ||||||
|---|---|---|---|---|---|---|---|---|---|
| 30 DAI | 60 DAI | Increase over 30 DAI (%) | 30 DAI | 60 DAI | Increase over 30 DAI (%) | 30 DAI | 60 DAI | Increase over 30 DAI (%) | |
| M1 | 2.1 b | 4.3 bc | 102 a | 2.8 ab | 3.9 cd | 37 b | 2.0 ab | 3.6 a | 80 d |
| M2 | 2.2 b | 4.3 bc | 97 b | 2.1 bc | 4.8 ab | 131 a | 1.2 b | 3.4 a | 183 a |
| M3 | 2.5 b | 4.9 a | 100 ab | 3.0 a | 5.3 a | 76 b | 1.6 ab | 3.2 a | 100 c |
| Means | 100 | 81 | 121 | ||||||
| M1 | 2.5 b | 4.7 ab | 88 b | 2.4 abc | 4.2 bc | 76 b | 1.6 ab | 4.0 a | 150 cd |
| M2 | 2.7 ab | 4.2 bc | 57 c | 2.5 ab | 4.2 bc | 687 b | 2.0 ab | 3.8 a | 90 abc |
| M3 | 3.2 a | 4.0 cd | 25 d | 2.2 abc | 3.7 cd | 66 b | 2.6 a | 3.6 a | 38 e |
| Means | 57 | 70 | 93 | ||||||
| M1 | 1.3 c | 2.5 e | 914 b | 1.5 c | 2.6 e | 73 b | 2.6 a | 3.6 a | 38 ae |
| M2 | 2.5 b | 3.5 d | 40 cd | 2.3 abc | 3.6 cd | 54 b | 2.2 ab | 1.6 a | −27 f |
| M3 | 2.7 ab | 3.8 cd | 40 cd | 2.7 ab | 3.4 d | 27 c | 2.6 a | 2.0 a | −23 f |
| Means | 57 | 51 | −4 | ||||||
| LSD (p ≤ 0.05) | |||||||||
| Embryo age (EA) | 0.21 | 0.21 | 8.03 | 0.31 | 0.24 | 9.50 | 0.41 | 0.88 | 4.11 |
| Media (M) | 0.21 | 0.21 | 8.03 | 0.31 | 0.24 | 9.50 | 0.41 | 0.88 | 4.11 |
| EA × M | 0.59 | 0.59 | 22.69 | 0.89 | 0.70 | 26.84 | 1.18 | NS | 11.61 |