<abstract xmlns="http://www.w3.org/1999/xhtml">In this study, the effects of compounds providing Fe in chelated (NaFeEDTA and Fe(III)AC) and non-chelated (FeSO4·7H2O) forms as components of culture media, on <italic>in vitro</italic> shoot multiplication and rooting of <italic>Magnolia soulangeana</italic> ‘Alexandrina’, <italic>Magnolia grandiflora</italic> and <italic>Prunus cerasifera</italic> ‘Nigra’ were comparatively evaluated. Each of the tested chemicals was used as a single Fe source in the basal salt medium. In the stages of shoot multiplication and rooting plant response was scored by biometrical indices (number of shoots, leaves and roots, shoot and root length, percent of rooted plants and root hairs). The occurrence of physiological disorders was estimated by visual observations. In presence of FeSO4, symptoms of chlorosis, hyperhy-dricity, early senescence and specific morphology of roots, suggesting Fe deficiency, were observed. These deteriorations were entirely prevented at the application of Fe chelates of which, in this experimental systems, Fe(III)AC was tested for the first time. The addition of Fe(III)AC positively affected the plant quality to extent comparable to that of NaFeEDTA. The obtained data suggest that both applied Fe chelates are more appropriate than non-chelated Fe form and can be alternatively used in the optimization of nutrient media for micropropagation of <italic>Magnolia</italic> and <italic>Prunus cerasifera</italic> genotypes.</abstract>

In this study, the effects of compounds providing Fe in chelated (NaFeEDTA and Fe(III)AC) and non-chelated (FeSO4·7H2O) forms as components of culture media, on in vitro shoot multiplication and rooting of Magnolia soulangeana ‘Alexandrina’, Magnolia grandiflora and Prunus cerasifera ‘Nigra’ were comparatively evaluated. Each of the tested chemicals was used as a single Fe source in the basal salt medium. In the stages of shoot multiplication and rooting plant response was scored by biometrical indices (number of shoots, leaves and roots, shoot and root length, percent of rooted plants and root hairs). The occurrence of physiological disorders was estimated by visual observations. In presence of FeSO4, symptoms of chlorosis, hyperhy-dricity, early senescence and specific morphology of roots, suggesting Fe deficiency, were observed. These deteriorations were entirely prevented at the application of Fe chelates of which, in this experimental systems, Fe(III)AC was tested for the first time. The addition of Fe(III)AC positively affected the plant quality to extent comparable to that of NaFeEDTA. The obtained data suggest that both applied Fe chelates are more appropriate than non-chelated Fe form and can be alternatively used in the optimization of nutrient media for micropropagation of Magnolia and Prunus cerasifera genotypes.

Influence Of Iron Sources In The Nutrient Medium On <italic>In Vitro</italic> Shoot Multiplication And Rooting Of Magnolia And Cherry Plum

Institute of Ornamental Plants, 1222 Negovan, Sofia, Bulgaria

Journal of Horticultural Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Influence Of Iron Sources In The Nutrient Medium On  Shoot Multiplication And Rooting Of Magnolia And Cherry Plum

In this study, the effects of compounds providing Fe in chelated (NaFeEDTA and Fe(III)AC) and non-chelated (FeSO4·7H2O) forms as components of culture media,...

Influence Of Iron Sources In The Nutrient Medium On In Vitro Shoot Multiplication And Rooting Of Magnolia And Cherry Plum

Published Online: Dec 30, 2015

Page range: 27 - 38

Received: Aug 01, 2015

Accepted: Nov 01, 2015

DOI: https://doi.org/10.2478/johr-2015-0014

Keywords
Ammonium iron (III) citrate, NaFeEDTA, FeSO, × ‘Alexandrina’, ‘Nigra’

© 2015 Rosen S. Sokolov et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Influence Of Iron Sources In The Nutrient Medium On In Vitro Shoot Multiplication And Rooting Of Magnolia And Cherry Plum

Published Online: Dec 30, 2015

Page range: 27 - 38

Received: Aug 01, 2015

Accepted: Nov 01, 2015

DOI: https://doi.org/10.2478/johr-2015-0014

KeywordsAmmonium iron (III) citrate, NaFeEDTA, FeSO, × ‘Alexandrina’, ‘Nigra’

© 2015 Rosen S. Sokolov et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
Ammonium iron (III) citrate, NaFeEDTA, FeSO, × ‘Alexandrina’, ‘Nigra’