<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The map of the linkage groups: LG2, LG12 and LG14, which are expected to contain QTLs related to fruit quality, was generated by analysis of 56 individuals of the cross: ‘Retina’ × ‘Topaz’. Twenty three of the 27 SSR markers covered 225 cM in ‘Retina’ and 371 cM in ‘Topaz’ genome. High level of colinearity (≈85%) was found between obtained map and the respective map regions of ‘Fiesta’, ‘Discovery’, ‘Ralls Janet’ and ‘Delicious’. Only single inversions of marker positions were noted, predominantly in ‘Topaz’. CAPS and SSCP/SNP markers in seven ESTs, chosen based on the metabolic pathways of ascorbic acids and sugar, were identified. Four of the CAPS markers, linked to the genes coding: <italic>UDP</italic> glucose: flavonoid 3-o-glucosyl transferase, ascorbate peroxidase, and two sugar transporters, were mapped on LG2 (GFglTra - both cultivars), LG12 (PGiso1B and PSTS - ‘Topaz’) and LG14 (PST - ‘Retina’). According to our knowledge, loci of these markers have never been identified on the apple genome map.</p></abstract>

The map of the linkage groups: LG2, LG12 and LG14, which are expected to contain QTLs related to fruit quality, was generated by analysis of 56 individuals of the cross: ‘Retina’ × ‘Topaz’. Twenty three of the 27 SSR markers covered 225 cM in ‘Retina’ and 371 cM in ‘Topaz’ genome. High level of colinearity (≈85%) was found between obtained map and the respective map regions of ‘Fiesta’, ‘Discovery’, ‘Ralls Janet’ and ‘Delicious’. Only single inversions of marker positions were noted, predominantly in ‘Topaz’. CAPS and SSCP/SNP markers in seven ESTs, chosen based on the metabolic pathways of ascorbic acids and sugar, were identified. Four of the CAPS markers, linked to the genes coding: UDP glucose: flavonoid 3-o-glucosyl transferase, ascorbate peroxidase, and two sugar transporters, were mapped on LG2 (GFglTra - both cultivars), LG12 (PGiso1B and PSTS - ‘Topaz’) and LG14 (PST - ‘Retina’). According to our knowledge, loci of these markers have never been identified on the apple genome map.

Identification of Four New Est-Based Markers on the Apple <italic>(M<sc>ALUS ⋉ DOMESTICA</sc>)</italic> Genetic Map

Research Institute of Horticulture Konstytucji 3 Maja 1/3, 96-100 Skierniewice, Poland

Journal of Horticultural Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Identification of Four New Est-Based Markers on the Apple  Genetic Map

The map of the linkage groups: LG2, LG12 and LG14, which are expected to contain QTLs related to fruit quality, was generated by analysis of 56 individuals of...

Identification of Four New Est-Based Markers on the Apple (MALUS ⋉ DOMESTICA) Genetic Map

Published Online: Sep 10, 2014

Page range: 93 - 100

Received: Dec 02, 2013

Accepted: May 30, 2014

DOI: https://doi.org/10.2478/johr-2014-0011

Keywords
comparative mapping, SSR transferability, ascorbic acid, sugar metabolism

© 2014 Sylwia Keller-Przybyłkowicz and Małgorzata Korbin

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Identification of Four New Est-Based Markers on the Apple (MALUS ⋉ DOMESTICA) Genetic Map

Published Online: Sep 10, 2014

Page range: 93 - 100

Received: Dec 02, 2013

Accepted: May 30, 2014

DOI: https://doi.org/10.2478/johr-2014-0011

Keywordscomparative mapping, SSR transferability, ascorbic acid, sugar metabolism

© 2014 Sylwia Keller-Przybyłkowicz and Małgorzata Korbin

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
comparative mapping, SSR transferability, ascorbic acid, sugar metabolism