<abstract xmlns="http://www.w3.org/1999/xhtml"><p><italic>Apis cerana</italic> Fabricius, 1793 is the eastern honeybee species distributed throughout Asia from the tropical climate in the southern part to the temperate climate in the northern part. We sequenced and annotated the complete mitochondrial DNA (mtDNA) of <italic>A. cerana</italic> from Vladivostok, Primorsky Krai of the Russian Far East and uploaded it to the database GenBank (AP018450). MtDNA sequence has 15,919 bp length, AT-content 84% and GC-content 16% and contains 22 tRNA genes, 13 protein-coding genes, two ribosomal RNA genes, one AT-rich region and four non-coding intergenic regions (NC1-4). All proteincoding genes start with ATT and ATG codons, except for ATC, the start codon of the ATP8 gene, which and stop with the common stop codons TAA and TAG. A comparative analysis of complete mtDNA of <italic>A. cerana</italic> from China, Indonesia, Korea, Malaysia, Russia, Taiwan, Thailand, Vietnam, and Japan found that the Russian Far East <italic>Apis cerana</italic> differed from others on the subspecies level. Based on the comparative analysis of complete mtDNA (~16,000 bp), nuclear DNA (nDNA) gene Vitellogenin (VG) (~4,100 bp) and morphological measurements (six parameters), we assumed that the Russian Far-East <italic>A. cerana</italic> can be a distinct northern Asia population and can be described as a separate unique subspecies of <italic>A. c. ussuriensis</italic> subsp. nov. <italic>A. c. koreana</italic> subsp. nov. is also validated and described as a new subspecies.</p></abstract>

Apis cerana Fabricius, 1793 is the eastern honeybee species distributed throughout Asia from the tropical climate in the southern part to the temperate climate in the northern part. We sequenced and annotated the complete mitochondrial DNA (mtDNA) of A. cerana from Vladivostok, Primorsky Krai of the Russian Far East and uploaded it to the database GenBank (AP018450). MtDNA sequence has 15,919 bp length, AT-content 84% and GC-content 16% and contains 22 tRNA genes, 13 protein-coding genes, two ribosomal RNA genes, one AT-rich region and four non-coding intergenic regions (NC1-4). All proteincoding genes start with ATT and ATG codons, except for ATC, the start codon of the ATP8 gene, which and stop with the common stop codons TAA and TAG. A comparative analysis of complete mtDNA of A. cerana from China, Indonesia, Korea, Malaysia, Russia, Taiwan, Thailand, Vietnam, and Japan found that the Russian Far East Apis cerana differed from others on the subspecies level. Based on the comparative analysis of complete mtDNA (~16,000 bp), nuclear DNA (nDNA) gene Vitellogenin (VG) (~4,100 bp) and morphological measurements (six parameters), we assumed that the Russian Far-East A. cerana can be a distinct northern Asia population and can be described as a separate unique subspecies of A. c. ussuriensis subsp. nov. A. c. koreana subsp. nov. is also validated and described as a new subspecies.

Phylogenetic Relationships of Russian Far-East <italic>Apis cerana</italic> with Other North Asian Populations

Institute of Biochemistry and Genetics, Russian Academy of Sciences

Division of Life Sciences, Major of Biological Sciences, Incheon National University Convergence Research Center for Insect Vectors, Incheon National University

Federal Scientific Center of the East Asia Terrestrial Biodiversity

Journal of Apicultural Science

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Phylogenetic Relationships of Russian Far-East  with Other North Asian Populations

Apis cerana Fabricius, 1793 is the eastern honeybee species distributed throughout Asia from the tropical climate in the southern part to the temperate climate...

Phylogenetic Relationships of Russian Far-East Apis cerana with Other North Asian Populations

Published Online: Dec 26, 2019

Page range: 289 - 314

Received: Feb 02, 2019

Accepted: Sep 03, 2019

DOI: https://doi.org/10.2478/jas-2019-0024

Keywords
mitochondrial genome, new subspecies, Russian Far East, vitellogenin

© 2019 Rustem A. Ilyasov et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Phylogenetic Relationships of Russian Far-East Apis cerana with Other North Asian Populations

Published Online: Dec 26, 2019

Page range: 289 - 314

Received: Feb 02, 2019

Accepted: Sep 03, 2019

DOI: https://doi.org/10.2478/jas-2019-0024

Keywordsmitochondrial genome, new subspecies, Russian Far East, vitellogenin

© 2019 Rustem A. Ilyasov et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
mitochondrial genome, new subspecies, Russian Far East, vitellogenin