Emerging evidence indicates that inflammation in atopic dermatitis (AD) is associated with immune-mediated abnormalities in the skin. The history and severity of AD are risk factors for dust mite allergy. Bee venom (BV) is used in a complementary medicine to treat various diseases and skin disorders. Purified BV is obtained through electric stunning with a BV collector, without the harming of honeybees, followed by the removal of impurities from the collected BV and lyophilization of the final product. To evaluate the therapeutic potential of purified BV for AD, we investigated the anti-inflammatory effects of BV on house dust mite (