<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Cyanobacteria are the rich resource of various secondary metabolites including toxins with broad pharmaceutical significance. The aim of this work was to evaluate the antiproliferative, neurotoxic, genotoxic and mutagenic effects of cyanobacterial extracts containing Microcystin-LR (MCLR) <italic>in vitro</italic>. ELISA analysis results showed that MCLR contents of five cyanobacterial extracts were 2.07 ng/mL, 1.43 ng/mL, 1.41 ng/mL, 1.27 ng/mL, and 1.12 ng/mL for <italic>Leptolyngbya</italic> sp. SB1, <italic>Phormidium</italic> sp. SB4, <italic>Oscillatoria earlei</italic> SB5, <italic>Phormidium</italic> sp. SB2, Uncultured <italic>cyanobacterium</italic>, respectively. <italic>Phormidium</italic> sp. SB4 and <italic>Phormidium</italic> sp. SB2 extracts had the lowest neurotoxicity (86% and 79% cell viability, respectively) and <italic>Oscillatoria earlei</italic> SB5 extracts had the highest neurotoxicity (47% cell viability) on PC12 cell at 1000 µg/ml extract concentration. <italic>Leptolyngbya</italic> sp. SB1 and <italic>Phormidium</italic> sp. SB2 showed the highest antiproliferative effect (92% and 77% cell death) on HT29 cell. On the other hand, all concentrations of five toxic cyanobacterial extracts induced DNA damage between 3.0% and 1.3% of tail intensity and did not cause any direct mutagenic effect at the 1000 µg/plate cyanobacterial extracts. These results suggest that cyanobacteria-derived MCLR is a promising candidate for development of effective agents against colon cancer.</p></abstract>

Cyanobacteria are the rich resource of various secondary metabolites including toxins with broad pharmaceutical significance. The aim of this work was to evaluate the antiproliferative, neurotoxic, genotoxic and mutagenic effects of cyanobacterial extracts containing Microcystin-LR (MCLR) in vitro. ELISA analysis results showed that MCLR contents of five cyanobacterial extracts were 2.07 ng/mL, 1.43 ng/mL, 1.41 ng/mL, 1.27 ng/mL, and 1.12 ng/mL for Leptolyngbya sp. SB1, Phormidium sp. SB4, Oscillatoria earlei SB5, Phormidium sp. SB2, Uncultured cyanobacterium, respectively. Phormidium sp. SB4 and Phormidium sp. SB2 extracts had the lowest neurotoxicity (86% and 79% cell viability, respectively) and Oscillatoria earlei SB5 extracts had the highest neurotoxicity (47% cell viability) on PC12 cell at 1000 µg/ml extract concentration. Leptolyngbya sp. SB1 and Phormidium sp. SB2 showed the highest antiproliferative effect (92% and 77% cell death) on HT29 cell. On the other hand, all concentrations of five toxic cyanobacterial extracts induced DNA damage between 3.0% and 1.3% of tail intensity and did not cause any direct mutagenic effect at the 1000 µg/plate cyanobacterial extracts. These results suggest that cyanobacteria-derived MCLR is a promising candidate for development of effective agents against colon cancer.

Antiproliferative, neurotoxic, genotoxic and mutagenic effects of toxic cyanobacterial extracts

Environmental Engineering, Faculty of Engineering-Architecture, Life sciences

Biology, Faculty of Science, Life sciences

Interdisciplinary Toxicology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Cyanobacteria are the rich resource of various secondary metabolites including toxins with broad pharmaceutical significance. The aim of this work was to...

Antiproliferative, neurotoxic, genotoxic and mutagenic effects of toxic cyanobacterial extracts

Published Online: Oct 18, 2019

Page range: 267 - 274

Received: Aug 15, 2017

Accepted: Jan 13, 2018

DOI: https://doi.org/10.2478/intox-2018-0026

Keywords
Cyanobacteria, Microcystin-LR, HT29 cells, HeLa cells, PC12 cells

© 2018 Interdisciplinary Toxicology, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Antiproliferative, neurotoxic, genotoxic and mutagenic effects of toxic cyanobacterial extracts

Published Online: Oct 18, 2019

Page range: 267 - 274

Received: Aug 15, 2017

Accepted: Jan 13, 2018

DOI: https://doi.org/10.2478/intox-2018-0026

KeywordsCyanobacteria, Microcystin-LR, HT29 cells, HeLa cells, PC12 cells

© 2018 Interdisciplinary Toxicology, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
Cyanobacteria, Microcystin-LR, HT29 cells, HeLa cells, PC12 cells