<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Washing soda has been identified as a precarious contaminant of the freshwater ponds and lakes, the natural habitat of <italic>Eunapius carteri</italic>. Treatment of sublethal concentrations of washing soda for 384 hours exhibited a significant decrease in the densities of blast like cells, small and large amoebocytes. The percentage occurrence of granular cells and archaeocytes yielded a marked increase against the experimental concentrations of washing soda. Washing soda mediated alterations in the differential cell densities of <italic>E. carteri</italic> indicative of a state of physiological stress and an undesirable shift in the cellular homeostasis of the organism distributed in polluted environment. Experimental exposure of washing soda yielded a significant increase in the cellular dimensions of large amoebocytes and archaeocytes. Prolonged treatment with washing soda presented a gross reduction in nonself surface adhesion efficacy of <italic>E. carteri</italic> cells. Experimental concentrations of washing soda resulted in a dose dependent increment in the frequencies of binucleation and micronucleation in the cells of <italic>E. carteri</italic>. The data were indicative of a high level of genotoxicity of washing soda in <italic>E. carteri</italic>. The present investigation provides an important information base in understanding the toxin induced chemical stress on the archaic immune defense of a primitive urmetazoa.</p></abstract>

Washing soda has been identified as a precarious contaminant of the freshwater ponds and lakes, the natural habitat of Eunapius carteri. Treatment of sublethal concentrations of washing soda for 384 hours exhibited a significant decrease in the densities of blast like cells, small and large amoebocytes. The percentage occurrence of granular cells and archaeocytes yielded a marked increase against the experimental concentrations of washing soda. Washing soda mediated alterations in the differential cell densities of E. carteri indicative of a state of physiological stress and an undesirable shift in the cellular homeostasis of the organism distributed in polluted environment. Experimental exposure of washing soda yielded a significant increase in the cellular dimensions of large amoebocytes and archaeocytes. Prolonged treatment with washing soda presented a gross reduction in nonself surface adhesion efficacy of E. carteri cells. Experimental concentrations of washing soda resulted in a dose dependent increment in the frequencies of binucleation and micronucleation in the cells of E. carteri. The data were indicative of a high level of genotoxicity of washing soda in E. carteri. The present investigation provides an important information base in understanding the toxin induced chemical stress on the archaic immune defense of a primitive urmetazoa.

Washing soda induced alteration of the differential cell count, nonself surface adhesion efficacy and nuclear morphology of the polyphenotypic cells of a freshwater sponge of India

Interdisciplinary Toxicology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Washing soda has been identified as a precarious contaminant of the freshwater ponds and lakes, the natural habitat of Eunapius carteri. Treatment of sublethal...

Washing soda induced alteration of the differential cell count, nonself surface adhesion efficacy and nuclear morphology of the polyphenotypic cells of a freshwater sponge of India

Published Online: Mar 02, 2019

Page range: 155 - 168

Received: Aug 25, 2017

Accepted: Jan 31, 2018

DOI: https://doi.org/10.2478/intox-2018-0012

Keywords
cellular adhesion, differential cell count, genotoxicity, sodium carbonate

© 2018 Interdisciplinary Toxicology, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Washing soda induced alteration of the differential cell count, nonself surface adhesion efficacy and nuclear morphology of the polyphenotypic cells of a freshwater sponge of India

Published Online: Mar 02, 2019

Page range: 155 - 168

Received: Aug 25, 2017

Accepted: Jan 31, 2018

DOI: https://doi.org/10.2478/intox-2018-0012

Keywordscellular adhesion, differential cell count, genotoxicity, sodium carbonate

© 2018 Interdisciplinary Toxicology, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
cellular adhesion, differential cell count, genotoxicity, sodium carbonate