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Genetic characterization of hydatid cysts of different intermediate hosts

W. M. Mousa
W. M. Mousa
, 
A. M. Abdel-Wahab
A. M. Abdel-Wahab
, 
M. El-Gameel Sohila
M. El-Gameel Sohila
 and 
O. A. Mahdy
O. A. Mahdy
   | Aug 05, 2020
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Article Category: Research Article
Published Online: Aug 05, 2020
Page range: 185 - 195
Received: Nov 19, 2019
Accepted: Jan 31, 2020
DOI: https://doi.org/10.2478/helm-2020-0031
Keywords
© 2020 W. M. Mousa, A. M. Abdel-Wahab, M. El-Gameel Sohila, O. A. Mahdy, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1

An ethidium bromide-stained agarose gel (1.5 %) showing the PCR amplification product of the ITSI gene of E. granulosus. Lane M: a 100-bp plus molecular size marker; lane –ve: negative control; lane 1: camel host DNA; lane 2: cattle host DNA; lane 3: sheep host DNA; lane 4: donkey host DNA; and lane 5: rabbit host DNA.
An ethidium bromide-stained agarose gel (1.5 %) showing the PCR amplification product of the ITSI gene of E. granulosus. Lane M: a 100-bp plus molecular size marker; lane –ve: negative control; lane 1: camel host DNA; lane 2: cattle host DNA; lane 3: sheep host DNA; lane 4: donkey host DNA; and lane 5: rabbit host DNA.

Fig. 2

Genetic relationship between an E. canadensis isolate from a camel HC (accession no.: MK460268) and the reference sequences of genotypes G1–G8 based on phylogenetic analysis of ITS1 nucleotide sequences.
Genetic relationship between an E. canadensis isolate from a camel HC (accession no.: MK460268) and the reference sequences of genotypes G1–G8 based on phylogenetic analysis of ITS1 nucleotide sequences.

Fig. 3

Genetic relationship between an E. canadensis isolate from a camel HC (accession no.: MK460269) and the reference sequences of genotypes G1–G8 based on phylogenetic analysis of ITS1 nucleotide sequences.
Genetic relationship between an E. canadensis isolate from a camel HC (accession no.: MK460269) and the reference sequences of genotypes G1–G8 based on phylogenetic analysis of ITS1 nucleotide sequences.

Fig. 4

Genetic relationship between an E. canadensis isolate from a camel HC (accession no.: MK460272) and the reference sequences of genotypes G1–G8 based on phylogenetic analysis of ITS1 nucleotide sequences.
Genetic relationship between an E. canadensis isolate from a camel HC (accession no.: MK460272) and the reference sequences of genotypes G1–G8 based on phylogenetic analysis of ITS1 nucleotide sequences.

Fig. 5

Genetic relationship between an E. granulosus s. s. isolate from a cattle HC (accession no.: MK460273) and the reference sequences of genotypes G1–G8 based on phylogenetic analysis of ITS1 nucleotide sequences.
Genetic relationship between an E. granulosus s. s. isolate from a cattle HC (accession no.: MK460273) and the reference sequences of genotypes G1–G8 based on phylogenetic analysis of ITS1 nucleotide sequences.

Fig. 6

Genetic relationship between an E. granulosus s. s. isolate from a sheep HC (accession no.: MK460270) and the reference sequences of genotypes G1–G8 based on phylogenetic analysis of ITS1 nucleotide sequences.
Genetic relationship between an E. granulosus s. s. isolate from a sheep HC (accession no.: MK460270) and the reference sequences of genotypes G1–G8 based on phylogenetic analysis of ITS1 nucleotide sequences.

Fig. 7

Genetic relationship between E. equinus isolates from donkeys (accession no.: MK460266) and the reference sequences of genotypes G1–G8 based on phylogenetic analysis of ITS1 nucleotide sequences.
Genetic relationship between E. equinus isolates from donkeys (accession no.: MK460266) and the reference sequences of genotypes G1–G8 based on phylogenetic analysis of ITS1 nucleotide sequences.

Fig. 8

Genetic relationship between an E. canadensis isolate from HCs of experimentally infected rabbits (accession no.: MK460267) and the reference sequences of genotypes G1–G8 based on phylogenetic analysis of ITS1 nucleotide sequences.
Genetic relationship between an E. canadensis isolate from HCs of experimentally infected rabbits (accession no.: MK460267) and the reference sequences of genotypes G1–G8 based on phylogenetic analysis of ITS1 nucleotide sequences.

Fig. 9

Genetic relationship between an E. canadensis isolate from HCs of experimentally infected rabbits (accession no.: MK460271) and the reference sequences of genotypes G1–G8 based on phylogenetic analysis of ITS1 nucleotide sequences.
Genetic relationship between an E. canadensis isolate from HCs of experimentally infected rabbits (accession no.: MK460271) and the reference sequences of genotypes G1–G8 based on phylogenetic analysis of ITS1 nucleotide sequences.

Fig. 10

Position of nucleotide substitution in the rabbit HC partial ITS1 nucleotide sequence. GRS: GenBank reference sequence; CHC: camel HC; RHC: rabbit HC.
Position of nucleotide substitution in the rabbit HC partial ITS1 nucleotide sequence. GRS: GenBank reference sequence; CHC: camel HC; RHC: rabbit HC.

Echinococcus species and GenBank accession numbers of the ITS1 gene used for phylogenetic analysis.

HC isolatesE. granulosusGenotype of isolateGenBank accession no. of isolateReference accession no. in GenBank
CamelE. canadensisG6MK460268AJ237775.1
CamelE. canadensisG6MK460269AJ237775.1
CamelE. canadensisG6MK460272KP866147.1
CattleE. granulosus s.sG1MK460273AY969044.1
SheepE. granulosus s.sG1MK460270KJ363926.1
DonkeyE. equinusG4MK460266AJ237773.1
RabbitE. canadensisG6MK460267AJ237775.1
RabbitE. canadensisG6MK460271KP866147.1
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