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The mouse bile duct tapeworm, Hymenolepis microstoma in free-living small mammals in Slovakia: occurrence and genetic analysis

J. Jarošová
J. Jarošová
, 
V. Šnábel
V. Šnábel
, 
S. Cavallero
S. Cavallero
, 
G. Chovancová
G. Chovancová
, 
Z. Hurníková
Z. Hurníková
 and 
D. Antolová
D. Antolová
   | May 23, 2020
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Published Online: May 23, 2020
Page range: 120 - 128
Received: Dec 19, 2019
Accepted: Feb 17, 2020
DOI: https://doi.org/10.2478/helm-2020-0022
Keywords
© 2020 J. Jarošová, V. Šnábel, S. Cavallero, G. Chovancová, Z. Hurníková, D. Antolová, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Fig. 1

Neighbor-joining (NJ) phylogram generated from the sequences of the paramyosine (pmy, 617 bp) partial gene showing the relationships among the SK-CS isolate from common shrew in Slovakia and GenBank-retrieved hymenolepididae sequences. Reference sequence for Hymenolepis microstoma (Hm) and H. citelli (Hc) were taken from the PhD thesis of Macnish (2001). Taenia solium cestode was used as the outgroup. Numbers next to the branches indicate the bootstrap value calculated from 1,000 replicates. Only bootstrap values >50% are shown at the branching points. Species involved in the phylogram: H. microstoma (Hm), H. nana (Hn), H. diminuta (Hd), H. citelli (Hc), Taenia solium (Ts). Geographical origins of the isolates: POR – Portugal, AUS – Australia, UK – United Kingdom, MEX – Mexico.
Neighbor-joining (NJ) phylogram generated from the sequences of the paramyosine (pmy, 617 bp) partial gene showing the relationships among the SK-CS isolate from common shrew in Slovakia and GenBank-retrieved hymenolepididae sequences. Reference sequence for Hymenolepis microstoma (Hm) and H. citelli (Hc) were taken from the PhD thesis of Macnish (2001). Taenia solium cestode was used as the outgroup. Numbers next to the branches indicate the bootstrap value calculated from 1,000 replicates. Only bootstrap values >50% are shown at the branching points. Species involved in the phylogram: H. microstoma (Hm), H. nana (Hn), H. diminuta (Hd), H. citelli (Hc), Taenia solium (Ts). Geographical origins of the isolates: POR – Portugal, AUS – Australia, UK – United Kingdom, MEX – Mexico.

Fig. 2

Neighbor-joining (NJ) phylogram generated from the sequences of the ribosomal internal transcribed spacer (ITS1, 632 bp) showing the relationships among SK-EH isolate from European hamster in Slovakia and GenBank-retrieved hymenolepididae sequences. Numbers next to the branches indicate the bootstrap value calculated from 1,000 replicates. Only bootstrap values >50% are shown at the branching points. Species involved in the phylogram: Hymenolepis microstoma (Hm), H. nana (Hn), H. diminuta (Hd), Rodentolepis straminea (Rs), Pseudoanoplocephala crawfordi (Pc), Staphylocystis furcata (Sf), Staphylocystis schilleri (Ss). Geographical origins of the isolates: AUS – Australia, POR – Portugal, SPA – Spain, IRA – Iran, UK - United Kindom, UKR – Ukraine, FRA – France, USA – United States of America, CHI – China.
Neighbor-joining (NJ) phylogram generated from the sequences of the ribosomal internal transcribed spacer (ITS1, 632 bp) showing the relationships among SK-EH isolate from European hamster in Slovakia and GenBank-retrieved hymenolepididae sequences. Numbers next to the branches indicate the bootstrap value calculated from 1,000 replicates. Only bootstrap values >50% are shown at the branching points. Species involved in the phylogram: Hymenolepis microstoma (Hm), H. nana (Hn), H. diminuta (Hd), Rodentolepis straminea (Rs), Pseudoanoplocephala crawfordi (Pc), Staphylocystis furcata (Sf), Staphylocystis schilleri (Ss). Geographical origins of the isolates: AUS – Australia, POR – Portugal, SPA – Spain, IRA – Iran, UK - United Kindom, UKR – Ukraine, FRA – France, USA – United States of America, CHI – China.

List of small mammal species collected from two localities in eastern and northern Slovakia.

LocalityAnimal speciesNo. of tested individuals
Tatra National Park and surroundingsBrown rat (Ratus norvegicus)1
House mouse (Mus musculus12
Striped field mouse (Apodemus agrarius)4
Yellow-necked mouse (Apodemus flavicollis)21
Common shrew (Sorex araneus)38
Eurasian pygmy shrew (Sorex minutus)14
Alpine shrew (Sorex alpinus)1
Mediterranean water shrew (Neomys anomalus)1
Common vole (Microtus arvalis)1
Field vole (Microtus agrestis)5
Bank vole (Clethrionomys glareolus)26
European mole (Talpa europaea)4
Brown rat (Ratus norvegicus)21
Košice city and Rozhanovce village and surroundingsHouse mouse (Mus musculus)7
European hamster (Cricetus cricetus)2
Eurasian harvest mouse (Micromys minutus)1
Hazel dormouse (Muscardinus avellanarius)1
Striped field mouse (Apodemus agrarius)2
Yellow-necked mouse (Apodemus flavicollis)2
Lesser shrew (Crocidura suaveolens)5
Eurasian pygmy shrew (Sorex minutus)3
Common shrew (Sorex araneus)2
Common vole (Microtus arvalis)4
European pine vole (Microtus subterraneus)7
European mole (Talpa europea)1
TOTAL186
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