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A controlled study on efficacy and egg reappearance period of Ivermectin in donkeys naturally infected with small strongyles


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Arias, M., Cazapal-Monteiro, C., Valderrábano, E., Miguélez, S., Rois, J.L., López-Arellano, M.E., Madeira de Carvalho, L., Mendoza de Gives, P., Sánchez-Andrade, R., Paz-Silva, A. (2013): A preliminary study of the biological control of strongyles affecting equids in a zoological park. J. Equine Vet. Sci. 33(12): 1115–1120. DOI: 10.1016/j.jevs.2013.04.013AriasM.Cazapal-MonteiroC.ValderrábanoE.MiguélezS.RoisJ.L.López-ArellanoM.E.Madeira de CarvalhoL.Mendoza de GivesP.Sánchez-AndradeR.Paz-SilvaA.2013A preliminary study of the biological control of strongyles affecting equids in a zoological parkJ. Equine Vet. Sci33121115112010.1016/j.jevs.2013.04.013Open DOISearch in Google Scholar

Bellaw, J.L., Krebs, K., Reinemeyer, C.R., Norris, J.K., Scare, J.A., Pagano, S., Nielsen, M.K. (2018): Anthelmintic therapy of equine cyathostomin nematodes – larvicidal efficacy, egg reappearance period, and drug resistance. Int. J. Parasitol. 48(2): 97–105. DOI: 10.1016/j.ijpara.2017.08.009BellawJ.L.KrebsK.ReinemeyerC.R.NorrisJ.K.ScareJ.A.PaganoS.NielsenM.K.2018Anthelmintic therapy of equine cyathostomin nematodes – larvicidal efficacy, egg reappearance period, and drug resistanceInt. J. Parasitol4829710510.1016/j.ijpara.2017.08.00929050919Open DOISearch in Google Scholar

Borioni, N., Marinaro, P., Celestini, S., Del Sole, F., Magro, R., Zoppi, D., Mattei, F., Dall’ Armi, V., Mazzarella, F., Cesario, A., Bonassi, S. (2012): Effect of equestrian therapy and onotherapy in physical and psycho-social performances of adults with intellectual disability: a preliminary study of evaluation tools based on the ICF classification. Disabil. Rehabil. 34(4): 279–287. DOI: 10.3109/09638288.2011.605919BorioniN.MarinaroP.CelestiniS.Del SoleF.MagroR.ZoppiD.MatteiF.Dall’ ArmiV.MazzarellaF.CesarioA.BonassiS.2012Effect of equestrian therapy and onotherapy in physical and psycho-social performances of adults with intellectual disability: a preliminary study of evaluation tools based on the ICF classificationDisabil. Rehabil34427928710.3109/09638288.2011.60591921988646Open DOISearch in Google Scholar

Bosco, A., Rinaldi, L., Maurelli, M.P., Musella, V., Coles, G.C., Cringoli, G. (2014): The comparison of FLOTAC, FECPAK and McMaster techniques for nematode egg counts in cattle. Acta Parasitol. 59(4): 625–628. DOI: 10.2478/s11686-014-0282-7BoscoA.RinaldiL.MaurelliM.P.MusellaV.ColesG.C.CringoliG.2014The comparison of FLOTAC, FECPAK and McMaster techniques for nematode egg counts in cattleActa Parasitol59462562810.2478/s11686-014-0282-725236271Open DOISearch in Google Scholar

Bu, Y., Niu, H., Gasser, R.B., Baveridge, I., Zhang, L. (2009): Strongyloid nematodes in the caeca of donkeys in Henan Province, China. Acta Parasitol. 54(3): 263–268. DOI: 10.2478/s11686-009-0037-zBuY.NiuH.GasserR.B.BaveridgeI.ZhangL.2009Strongyloid nematodes in the caeca of donkeys in Henan Province, ChinaActa Parasitol54326326810.2478/s11686-009-0037-zOpen DOISearch in Google Scholar

Buono, F., Roncoroni, C., Pacifico, L., Piantedosi, D., Neola, B., Barile, V.L., Fagiolo, A., Várady, M., Veneziano, V. (2018): Cyathostominae egg reappearance period after treatment with major horse anthelmintics in donkeys. J. Equine Vet. Sci. 65: 6–11. DOI: 10.1016/j.jevs.2018.02.018BuonoF.RoncoroniC.PacificoL.PiantedosiD.NeolaB.BarileV.L.FagioloA.VáradyM.VenezianoV.2018Cyathostominae egg reappearance period after treatment with major horse anthelmintics in donkeysJ. Equine Vet. Sci6561110.1016/j.jevs.2018.02.018Open DOISearch in Google Scholar

Canever, R.J., Braga, P.R., Boeckh, A., Grycajuck, M., Bier, D., Molento, M.B. (2013): Lack of Cyathostomin sp. reduction after anthelmintic treatment in horses in Brazil. Vet. Parasitol. 194(1): 35–39. DOI: 10.1016/j.vetpar.2012.12.020CaneverR.J.BragaP.R.BoeckhA.GrycajuckM.BierD.MolentoM.B.2013Lack of Cyathostomin spreduction after anthelmintic treatment in horses in Brazil. Vet. Parasitol1941353910.1016/j.vetpar.2012.12.02023318166Open DOISearch in Google Scholar

Cernea, M., Madeira, de Carvalho, L.M., Cozma, V. (2008): Atlas de diagnostic al strongilidozelor la ecvine [Atlas of diagnosis of equine Strongylidosis]. Cluj-Napoca, RO, Academic Press, 118 pp (In Romanian)CerneaM.Madeirade Carvalho, L.M.CozmaV.2008Atlas de diagnostic al strongilidozelor la ecvine [Atlas of diagnosis of equine Strongylidosis]Cluj-Napoca, ROAcademic Press118pp (In Romanian)Search in Google Scholar

Dibaba, M.D., Getachew, A.M., Assefa, Z., Fanta, A., Etana, M., Firew, S., Goshu, L., Burden, F. (2017): Seasonal variation of strongylosis in working donkeys of Ethiopia: a cross-sectional and longitudinal studies. Parasitol. Res. 116 (7): 2009–2015. DOI: 10.1007/s00436-017-5485-zDibabaM.D.GetachewA.M.AssefaZ.FantaA.EtanaM.FirewS.GoshuL.BurdenF.2017Seasonal variation of strongylosis in working donkeys of Ethiopia: a cross-sectional and longitudinal studiesParasitol. Res11672009201510.1007/s00436-017-5485-z28536880Open DOISearch in Google Scholar

Fangama, M.I., Seri, H.I., Suliman, S.E., Imam, S.M.A., Mozamel, E.A. (2013): Comparative efficacy evaluation of moxidectin and ivermectin injectable formulation against helminths infestation of donkeys Equus asinus in Sudan. Assiut Vet. Med. J. 59(137): 1–8. Getachew, M., Trawford, A., Feseha, G., Reid, S.W.J. (2010): Gastrointestinal parasites of working donkeys of Ethiopia. Trop. Anim. Health Prod. 42(1): 27–33. DOI: 10.1007/s11250-009-9381-0FangamaM.I.SeriH.I.SulimanS.E.ImamS.M.A.MozamelE.A.2013Comparative efficacy evaluation of moxidectin and ivermectin injectable formulation against helminths infestation of donkeys Equus asinus in SudanAssiut Vet. Med. J5913718Getachew, M., Trawford, A., Feseha, G., Reid, S.W.J. (2010): Gastrointestinal parasites of working donkeys of Ethiopia. Trop. Anim. Health Prod. 42(1): 27–3310.1007/s11250-009-9381-019548106Open DOISearch in Google Scholar

Grosenbaugh, G.A., Reinemeyer, C.R., Figueiredo, M.D. (2011). Pharmacology and therapeutics in donkeys. Equine Vet. Educ. 23(10): 523–530. DOI: 10.1111/j.2042-3292.2011.00291.xGrosenbaughG.A.ReinemeyerC.R.FigueiredoM.D.2011Pharmacology and therapeutics in donkeysEquine Vet. Educ231052353010.1111/j.2042-3292.2011.00291.xOpen DOISearch in Google Scholar

Imam, S.M.A., Seri, H.I., Hassan, T., Tigani, T.A., Zolain, H.B., Abakar, A.D. (2010): Therapeutic efficacy evaluation of anthelmintics activity of albendazole and ivermectin drench formulations in donkeys in Darfur, Sudan. Vet. Arhiv 80(5): 585–595ImamS.M.A.SeriH.I.HassanT.TiganiT.A.ZolainH.B.AbakarA.D.2010Therapeutic efficacy evaluation of anthelmintics activity of albendazole and ivermectin drench formulations in donkeys in Darfur, SudanVet. Arhiv805585595Search in Google Scholar

Ismail, A.A., Ahmed, N.K., Bashar, A.E., Seri, H.I., El Tigani-Asil, E.T.A., Abakar, A.D. (2016): A survey of seasonal gastrointestinal parasitic infections in donkeys from a semiarid sub-Saharan region, Sudan. J. Pathog. 2016: 4602751. DOI: 10.1155/2016/4602751IsmailA.A.AhmedN.K.BasharA.E.SeriH.I.El Tigani-AsilE.T.A.AbakarA.D.2016A survey of seasonal gastrointestinal parasitic infections in donkeys from a semiarid sub-Saharan region, SudanJ. Pathog2016460275110.1155/2016/4602751Open DOISearch in Google Scholar

Jajere, S.M., Lawal, J.R., Bello, A.M., Wakil, Y., Turaki, U.A., Waziri, I. (2016): Risk factors associated with the occurrence of gastrointestinal helminths among indigenous donkeys Equus asinus in Northeastern Nigeria. Scientifica (Cairo) 2016: 3735210. DOI: 10.1155/2016/3735210JajereS.M.LawalJ.R.BelloA.M.WakilY.TurakiU.A.WaziriI.2016Risk factors associated with the occurrence of gastrointestinal helminths among indigenous donkeys Equus asinus in Northeastern NigeriaScientifica (Cairo)2016373521010.1155/2016/3735210Open DOISearch in Google Scholar

Kaplan, R.M., Nielsen, M.K. (2010): An evidence-based approach to equine parasite control: it ain’t the 60s anymore Equine Vet. Educ. 22(6): 306–316. DOI: 10.1111/j.2042-3292.2010.00084.xKaplanR.M.NielsenM.K.2010An evidence-based approach to equine parasite control: it ain’t the 60s anymoreEquine Vet. Educ22630631610.1111/j.2042-3292.2010.00084.xOpen DOISearch in Google Scholar

Kornaś, S., Gawor, J., Cabaret, J., Molenda, K., Skalsk a, M., Nowosad, B. (2009): Morphometric identification of equid cyathostome (Nematoda: Cyathostominae) infective larvae. Vet. Parasitol. 162 (3-4): 290–294. DOI: 10.1016/j.vetpar.2009.03.018KornaśS.GaworJ.CabaretJ.MolendaK.Skalska, M.NowosadB.2009Morphometric identification of equid cyathostome (Nematoda: Cyathostominae) infective larvaeVet. Parasitol1623-429029410.1016/j.vetpar.2009.03.018Open DOISearch in Google Scholar

Larsen, M.L., Ritz, C., Petersen, S.L., Nielsen, M.K. (2011): Determination of ivermectin efficacy against cyathostomins and Parascaris equorum on horse farms using selective therapy. Vet. J. 188(1): 44–47. DOI: 10.1016/j.tvjl.2010.03.009.LarsenM.L.RitzC.PetersenS.L.NielsenM.K.2011Determination of ivermectin efficacy against cyathostomins and Parascaris equorum on horse farms using selective therapyVet. J1881444710.1016/j.tvjl.2010.03.009Open DOISearch in Google Scholar

Lawson, E., Burden, F., Elsheikha, H.M. (2015): Pyrantel resistance in two herds of donkey in the UK. Vet. Parasitol. 207(3-4): 346–349. DOI: 10.1016/j.vetpar.2014LawsonE.BurdenF.ElsheikhaH.M.2015Pyrantel resistance in two herds of donkey in the UKVet. Parasitol2073-434634910.1016/j.vetpar.2014Open DOISearch in Google Scholar

Little, D., Flowers, J.R., Hamerberg, B.H., Gardner, S.Y. (2013): Management of drug resistant cyathostominosis on a breeding farm in central North Carolina. Equine Vet. J. 35(3): 246–251. DOI: 10.2746/042516403776148264LittleD.FlowersJ.R.HamerbergB.H.GardnerS.Y.2013Management of drug resistant cyathostominosis on a breeding farm in central North CarolinaEquine Vet. J35324625110.2746/042516403776148264Open DOISearch in Google Scholar

Mao, X., Gu, J., Sun, Y., Xu, S., Zhang, X., Yang, H., Ren, F. (2009): Anti-proliferative and anti-tumour effect of active components in donkey milk on A549 human lung cancer cells. Int. Dairy J. 19(11): 703–708. DOI: 10.1016/j.idairyj.2009.05.007MaoX.GuJ.SunY.XuS.ZhangX.YangH.RenF.2009Anti-proliferative and anti-tumour effect of active components in donkey milk on A549 human lung cancer cellsInt. Dairy J191170370810.1016/j.idairyj.2009.05.007Open DOISearch in Google Scholar

MAFF (1986): Manual of veterinary parasitological laboratory techniques 3rd Edition, London, UK, H.M.S.O., pp 160.MAFF1986Manual of veterinary parasitological laboratory techniques3rd EditionLondon, UK, H.M.S.O.160Search in Google Scholar

Matthee, S., Krecek, R.C., Milne, S.A. (2000): Prevalence and biodiversity of helminth parasites in donkeys from South Africa. J. Parasitol. 86(4): 756–762. DOI: 10.1645/0022-3395(2000)086[0756:PABOHP]2.0.CO;2MattheeS.KrecekR.C.MilneS.A.2000Prevalence and biodiversity of helminth parasites in donkeys from South AfricaJ. Parasitol86475676210.1645/0022-3395(2000)086[0756:PABOHP]2.0.CO;2Open DOISearch in Google Scholar

Matthee, S., Krecek, R.C., Milne, S.A., Boshof, M., Guthrie, A.J. (2002): Impact of management interventions on helminth levels, and body and blood measurements in working donkeys in South Africa. Vet. Parasitol. 107(1-2): 103–113. DOI: 10.1016/S0304-4017(02)00113-9MattheeS.KrecekR.C.MilneS.A.BoshofM.GuthrieA.J.2002Impact of management interventions on helminth levels, and body and blood measurements in working donkeys in South AfricaVet. Parasitol1071-210311310.1016/S0304-4017(02)00113-9Open DOISearch in Google Scholar

Matthews, J.B., Burden, F.A. (2013): Common helminth infections of donkeys and their control in temperate regions. Equine Vet. Educ. 25(9): 461–467. DOI: 10.1111/eve.12018MatthewsJ.B.BurdenF.A.2013Common helminth infections of donkeys and their control in temperate regionsEquine Vet. Educ25946146710.1111/eve.12018Open DOISearch in Google Scholar

McFarlane, D., Hale, G.M., Johnson, E.M., Maxwell, L.K. (2010): Fecal egg counts after anthelmintic administration to aged horses and horses with pituitary pars intermedia dysfunction J. Am. Vet. Med. Assoc. 23(3): 330–334. DOI: 10.2460/javma.236.3.330McFarlaneD.HaleG.M.JohnsonE.M.MaxwellL.K.2010Fecal egg counts after anthelmintic administration to aged horses and horses with pituitary pars intermedia dysfunctionJ. Am. Vet. Med. Assoc23333033410.2460/javma.236.3.330Open DOISearch in Google Scholar

Mercier, P., Chick, B., Alves-Branco, F., White, C.R. (2001): Comparative efficacy, persistent effect, and treatment intervals of anthelmintic pastes in naturally infected horses. Vet. Parasitol. 99(1): 29–39. DOI: 10.1016/S0304-4017(01)00453-8MercierP.ChickB.Alves-BrancoF.WhiteC.R.2001Comparative efficacy, persistent effect, and treatment intervals of anthelmintic pastes in naturally infected horsesVet. Parasitol991293910.1016/S0304-4017(01)00453-8Open DOISearch in Google Scholar

Molena, R.A., Peachey, L.E., Di Cesare, A., Traversa, D., Cantacessi, C. (2018): Cyathostomine egg reappearance period following ivermectin treatment in a cohort of UK Thoroughbreds. Parasit. Vectors 11(1): 61. DOI: 10.1186/s13071-018-2638-6MolenaR.A.PeacheyL.E.Di CesareA.TraversaD.CantacessiC.2018Cyathostomine egg reappearance period following ivermectin treatment in a cohort of UK ThoroughbredsParasit. Vectors1116110.1186/s13071-018-2638-6578588729370872Open DOISearch in Google Scholar

Nielsen, M.K., Mittel, L., Grice, A., Erskine, M., Graves, E., Vala, W., Tully. R.C., French, D.D., Bowman, R., Kaplan, R.M. (2013): AAEP parasite control guidelines. American association of equine practitioners. Retrieved September 7, 2019 from https://aaep.org/sites/default/files/Documents/InternalParasiteGuidelines-Final5.23.19.pdf.NielsenM.K.MittelL.GriceA.ErskineM.GravesE.ValaW.TullyR.C.FrenchD.D.BowmanR.KaplanR.M.2013AAEP parasite control guidelinesAmerican association of equine practitioners. Retrieved September72019fromhttps://aaep.org/sites/default/files/Documents/InternalParasiteGuidelines-Final5.23.19.pdfSearch in Google Scholar

Nielsen, M.K., Vidyashankar, A., Andersen, U., De Lisi, K., Pilegard, K., Kaplan, R. (2010): Effects of fecal collection and storage factors on strongylid egg counts in horses. Vet. Parasitol. 167(1): 55–61. DOI: 10.1016/j.vetpar.2009.09.043NielsenM.K.VidyashankarA.AndersenU.De LisiK.PilegardK.KaplanR.2010Effects of fecal collection and storage factors on strongylid egg counts in horsesVet. Parasitol1671556110.1016/j.vetpar.2009.09.04319850412Open DOISearch in Google Scholar

Oryan, A., Farjani, Kish, G., Rajabloo, M. (2015): Larval cyathostominosis in a working donkey. J. Parasit. Dis. 39(2): 324–327. DOI: 10.1007/s12639-013-0313-6OryanA.FarjaniKish, G.RajablooM.2015Larval cyathostominosis in a working donkeyJ. Parasit. Dis39232432710.1007/s12639-013-0313-6445654626064028Open DOISearch in Google Scholar

Polidori, P., Vincenzetti, S. (2013): Use of donkey milk in children with cow’s milk protein allergy. Foods 2(2): 151–159. DOI: 10.3390/foods2020151PolidoriP.VincenzettiS.2013Use of donkey milk in children with cow’s milk protein allergyFoods2215115910.3390/foods2020151530226228239105Open DOISearch in Google Scholar

Polidori, P., Vincenzetti, S., Cavalluci, C., Beghelli, D. (2008): Quality of donkey meat and carcass characteristics. Meat Sci. 80(4): 1222–1224. DOI: 10.1016/j.meatsci.2008.05.027PolidoriP.VincenzettiS.CavalluciC.BeghelliD.2008Quality of donkey meat and carcass characteristicsMeat Sci8041222122410.1016/j.meatsci.2008.05.02722063861Open DOISearch in Google Scholar

Rinaldi, L., Levecke, B., Bosco, A., Ianniello, D., Pepe, P., Charlier, J., Cringoli, G., Vercruysse, J. (2014): Comparison of individual and pooled faecal samples in sheep for the assessment of gastrointestinal strongyle infection intensity and anthelmintic drug efficacy using McMaster and Mini-FLOTAC. Vet. Parasitol. 205(1-2): 216–223. DOI: 10.1016/j.vetpar.2014.06.011RinaldiL.LeveckeB.BoscoA.IannielloD.PepeP.CharlierJ.CringoliG.VercruysseJ.2014Comparison of individual and pooled faecal samples in sheep for the assessment of gastrointestinal strongyle infection intensity and anthelmintic drug efficacy using McMaster and Mini-FLOTACVet. Parasitol2051-221622310.1016/j.vetpar.2014.06.01125002307Open DOISearch in Google Scholar

Saeed, M.A., Beveridge, I., Abbas, G., Beasley, A., Bauquier, J., Wilkes, E., Jacobson, C., Hughes, K.J., El-Hage, C., O’Handley, R., Hurley, J., Cudmore, L., Carrigan, P., Walter, L., Tennent-Brown, B., Nielsen, M.K., Jabbar, A. (2019): Systematic review of gastrointestinal nematodes of horses from Australia. Parasit Vectors 12(1): 188. DOI: 10.1186/s13071-019-3445-4SaeedM.A.BeveridgeI.AbbasG.BeasleyA.BauquierJ.WilkesE.JacobsonC.HughesK.J.El-HageC.O’HandleyR.HurleyJ.CudmoreL.CarriganP.WalterL.Tennent-BrownB.NielsenM.K.JabbarA.2019Systematic review of gastrointestinal nematodes of horses from AustraliaParasit Vectors12118810.1186/s13071-019-3445-4648919931036059Open DOISearch in Google Scholar

Seri, H.I., Abakar, A.D., Ismail, A.A., Tigani, T.A. (2005): Efficacy of Ivermectin in an injectable formulation against gastrointestinal nematodes of donkeys Equus asinusVet. Arhiv 75(4): 369–374 Tarigo-Martinie, J.L., Wyatt, A.R., Kaplan, R.M. (2001): Prevalence and clinical implications of anthelmintic resistance in cyathostomes of horses. J. Am. Vet. Med. Assoc. 218(12): 1957–1960. DOI: 15;218(12):1957-60SeriH.I.AbakarA.D.IsmailA.A.TiganiT.A.2005Efficacy of Ivermectin in an injectable formulation against gastrointestinal nematodes of donkeys Equus asinusVet. Arhiv754369374Tarigo-Martinie, J.L., Wyatt, A.R., Kaplan, R.M. (2001): Prevalence and clinical implications of anthelmintic resistance in cyathostomes of horses. J. Am. Vet. Med. Assoc. 218(12): 1957–1960. DOI: 15;218(12):1957-6010.2460/javma.2001.218.1957Search in Google Scholar

Trawford, A.F., Burden, F.A., Hodgkinson, J. (2005): Suspected moxidectin resistance in cyathostomes in two donkey herds at The Donkey Sanctuary, UK. In Proceedings of the 20th International Conference of the World Association for the Advancement of Veterinary ParasitologyOctober 16-20, 2005 Christchurch, NZ, 2005, p 196TrawfordA.F.BurdenF.A.HodgkinsonJ.2005Suspected moxidectin resistance in cyathostomes in two donkey herds at The Donkey Sanctuary, UKInProceedings of the 20th International Conference of the World Association for the Advancement of Veterinary Parasitology October 16-20, 2005Christchurch, NZ2005196Search in Google Scholar

Van Doorn, D.C.K., Eysker, M., Kooyman, F.N.J., Wagenar, J.A., Ploeger, H.W. (2012): Searching for ivermectin resistance in Dutch horses. Vet. Parasitol. 185(2-4): 355–358. DOI: 10.1016/j.vetpar.2011.09.02Van DoornD.C.K.EyskerM.KooymanF.N.J.WagenarJ.A.PloegerH.W.2012Searching for ivermectin resistance in Dutch horsesVet. Parasitol1852-435535810.1016/j.vetpar.2011.09.02Open DOISearch in Google Scholar

Waqas, M., Nawaz, M., Sajid, M., Ahmad, Z., Mushtaq, A., Jabbar, A., Zubair, M. (2015): Strongylosis (red worms infestation); a potential threat to donkey’s health and performance. Glob. Vet. 14(3): 345–350. DOI: 10.5829/idosi.gv.2015.14.03.9340WaqasM.NawazM.SajidM.AhmadZ.MushtaqA.JabbarA.ZubairM.2015Strongylosis (red worms infestation); a potential threat to donkey’s health and performanceGlob. Vet14334535010.5829/idosi.gv.2015.14.03.9340Open DOISearch in Google Scholar

Yoseph, S., Smith, D.G., Mengistu, A., Teklu, F., Firew, T., Betere, Y. (2005): Seasonal variation in the parasite burden and body condition of working donkeys in East Shewa and West Shewa regions of Ethiopia. Trop. Anim. Health Prod. 37 (Suppl. 1): 35–45. DOI: 10.1007/s11250-005-9004YosephS.SmithD.G.MengistuA.TekluF.FirewT.BetereY.2005Seasonal variation in the parasite burden and body condition of working donkeys in East Shewa and West Shewa regions of EthiopiaTrop. Anim. Health Prod37Suppl. 1354510.1007/s11250-005-9004Open DOISearch in Google Scholar

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