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Comparative study among lactophenol blue, lactophenol solution and proteinase-K lytic solution for rostellar hooks morphometry of Echinococcus granulosus protoscolices

S. J. Hammad
S. J. Hammad
, 
S. Cavallero
S. Cavallero
, 
F. S. Al-Nasiri
F. S. Al-Nasiri
 and 
S. D᾿ Amelio
S. D᾿ Amelio
   | Jan 25, 2020
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Article Category: Research Note
Published Online: Jan 25, 2020
Page range: 63 - 70
Received: Nov 15, 2019
Accepted: Dec 13, 2019
DOI: https://doi.org/10.2478/helm-2020-0010
Keywords
© 2020 S. J. Hammad, S. Cavallero, F. S. Al-Nasiri, S. D᾿Amelio, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Fig.1

Microscopic photography of protoscolices at different magnification (a, b) before use of lytic solutions (zero time), showing aggregation of protoscolices within the preservation solution (ethanol 70%).
Microscopic photography of protoscolices at different magnification (a, b) before use of lytic solutions (zero time), showing aggregation of protoscolices within the preservation solution (ethanol 70%).

Fig. 2

Microscopic photography of protoscolices after 30 min from zero time of addition of lactophenol blue (a), lactophenol solution (b), proteinase-K lytic solution (c, d).
Microscopic photography of protoscolices after 30 min from zero time of addition of lactophenol blue (a), lactophenol solution (b), proteinase-K lytic solution (c, d).

Fig. 3

Microscopic photography of protoscolices after 60 min from zero time of addition of lactophenol blue (a), lactophenol solution (b), proteinase-K lytic solution (c, d).
Microscopic photography of protoscolices after 60 min from zero time of addition of lactophenol blue (a), lactophenol solution (b), proteinase-K lytic solution (c, d).

Fig. 4

Microscopic photography of protoscolices after 90 min from zero time of addition of lactophenol blue (a), lactophenol solution (b), proteinase-K lytic solution (c, d).
Microscopic photography of protoscolices after 90 min from zero time of addition of lactophenol blue (a), lactophenol solution (b), proteinase-K lytic solution (c, d).

Fig. 5

Microscopic photography of protoscolices after 120 min from zero time of addition of lactophenol blue (a), lactophenol solution (b), proteinase-K lytic solution (c, d); arrows indicated the sites of lysis in cellular membrane.
Microscopic photography of protoscolices after 120 min from zero time of addition of lactophenol blue (a), lactophenol solution (b), proteinase-K lytic solution (c, d); arrows indicated the sites of lysis in cellular membrane.

Fig. 6

Microscopic photography of protoscolices after 150 min from zero time of addition of lactophenol blue (a), lactophenol solution (b), proteinase-K lytic solution (c, d).
Microscopic photography of protoscolices after 150 min from zero time of addition of lactophenol blue (a), lactophenol solution (b), proteinase-K lytic solution (c, d).

Fig. 7

Microscopic photography of protoscolices after 240 min from zero time of addition of lactophenol blue (a), lactophenol solution (b), proteinase-K lytic solution (c, d), arrows indicated the sites of lysis in cellular membrane.
Microscopic photography of protoscolices after 240 min from zero time of addition of lactophenol blue (a), lactophenol solution (b), proteinase-K lytic solution (c, d), arrows indicated the sites of lysis in cellular membrane.

Fig. 8

Microscopic photography of hooks after lysis the membrane of protoscolices after 240 min from zero time of addition of proteinase-K lytic solution to the protoscolices.
Microscopic photography of hooks after lysis the membrane of protoscolices after 240 min from zero time of addition of proteinase-K lytic solution to the protoscolices.

Clustering behavior of protoscolices and visibility of components at different times after treated with lactophenol-based solutions and Proteinase-K lytic solution.

Time of exposureLactophenol blue or lactophenol solutionProteinase-K lytic solution
Time 30 minutesProtoscolices aggregated/ Components and rostellar hooks not visible (Fig. 2a-b)Protoscolices separated/ clearer vision of components (Fig. 2c-d)
Time 60 minutesProtoscolices aggregated/ Components and rostellar hooks not visible (Fig. 3a-b)Protoscolices separated/ clearer vision of components (Fig. 3c-d)
Time 90 minutesProtoscolices aggregated/ clearer vision of components (Fig. 4a-b)Protoscolices separated/ vision of components same as time 60 (Fig. 4c-d)
Time 120 minutesProtoscolices partially separated/ components visible but with no lysis of membrane (Fig. 5a-b)Protoscolices well separated/ starting of membrane lysis (Fig. 5c-d)
Time 150 minutesProtoscolices partially separated/ components visible but with no lysis of membrane (Fig. 6a-b)Protoscolices well separated/ increasing of membrane lysis (Fig. 6c-d)
Time 240 minutesProtoscolices partially separated/ components visible (better with lactophenol) but with no lysis of membrane (Fig. 7a-b)Protoscolices well separated/ membrane lysis semi-completed (Fig. 7c-d) Rostellar hooks free and visible (Fig. 8)
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