<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Two species of digenetic trematodes of the genus <italic>Pleurogenoides viz</italic>., <italic>P. cyanophlycti</italic><xref ref-type="bibr" rid="j_helm-2020-0006_ref_025_w2aab2b8c24b1b7b1ab2b2c25Aa">Shinad &amp; Prasadan (2018a)</xref> and <italic>P. euphlycti</italic><xref ref-type="bibr" rid="j_helm-2020-0006_ref_026_w2aab2b8c24b1b7b1ab2b2c26Aa">Shinad &amp; Prasadan (2018b)</xref> have been described from India. Information regarding the molecular data of various species of the genus <italic>Pleurogenoides</italic> Travassos, 1921 is virtually lacking. This study addresses the application of molecular markers to validate the phylogenetic position of <italic>P. cyanophlycti</italic> and <italic>P. euphlycti</italic>. In the present study, two species <italic>P. cyanophlycti</italic> and <italic>P. euphlycti</italic> were collected between January 2016 to October 2017, infecting the freshwater frogs inhabiting the Western Ghats, India. In the present study, the two species were identifi ed morphologically and by PCR amplification of the 28S ribosomal RNA gene. Phylogenetic tree results clearly demonstrate that both <italic>P. cyanophlycti</italic> and <italic>P. euphlycti</italic> belongs to the family Pleurogenidae <xref ref-type="bibr" rid="j_helm-2020-0006_ref_018_w2aab2b8c24b1b7b1ab2b2c18Aa">Looss, 1899</xref>. Based on these results, we presented and discussed the phylogenetic relationships of <italic>P. cyanophlycti</italic> and <italic>P. euphlycti</italic> within family Pleurogenidae from India. Phylogenetic analyses showed that <italic>P. cyanophlycti</italic> and <italic>P. euphlycti</italic> cluster according to their vertebrate host and revealed an important congruence between the phylogenetic trees of <italic>Pleurogenoides</italic> and of their vertebrate hosts. <italic>P. cyanophlycti</italic> and <italic>P. euphlycti</italic> clearly constitute a separate, sister branch with other species of the genera, <italic>Pleurogenoides, Pleurogenes</italic> (<italic>=Candidotrema</italic>)<italic>, Prosotocus</italic> and <italic>Brandesia</italic>. The present study firstly provides important information about the molecular study and phylogenetic analysis of <italic>P. cyanophlycti</italic> and <italic>P. euphlycti</italic>. This study will also serve as a baseline for <italic>Pleurogenoides</italic> species identifi cation for further studies.</p></abstract>

Two species of digenetic trematodes of the genus Pleurogenoides viz., P. cyanophlyctiShinad & Prasadan (2018a) and P. euphlyctiShinad & Prasadan (2018b) have been described from India. Information regarding the molecular data of various species of the genus Pleurogenoides Travassos, 1921 is virtually lacking. This study addresses the application of molecular markers to validate the phylogenetic position of P. cyanophlycti and P. euphlycti. In the present study, two species P. cyanophlycti and P. euphlycti were collected between January 2016 to October 2017, infecting the freshwater frogs inhabiting the Western Ghats, India. In the present study, the two species were identifi ed morphologically and by PCR amplification of the 28S ribosomal RNA gene. Phylogenetic tree results clearly demonstrate that both P. cyanophlycti and P. euphlycti belongs to the family Pleurogenidae Looss, 1899. Based on these results, we presented and discussed the phylogenetic relationships of P. cyanophlycti and P. euphlycti within family Pleurogenidae from India. Phylogenetic analyses showed that P. cyanophlycti and P. euphlycti cluster according to their vertebrate host and revealed an important congruence between the phylogenetic trees of Pleurogenoides and of their vertebrate hosts. P. cyanophlycti and P. euphlycti clearly constitute a separate, sister branch with other species of the genera, Pleurogenoides, Pleurogenes (=Candidotrema), Prosotocus and Brandesia. The present study firstly provides important information about the molecular study and phylogenetic analysis of P. cyanophlycti and P. euphlycti. This study will also serve as a baseline for Pleurogenoides species identifi cation for further studies.

Phylogenetic position of <italic>Pleurogenoides</italic> species (Plagiorchiida: Pleurogenidae) from the duodenum of Indian skipper frog, <italic>Euphlyctis cyanophlyctis</italic> (Amphibia: Dicroglossidae) inhabiting the Western Ghats, India

Helminthologia

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Phylogenetic position of  species (Plagiorchiida: Pleurogenidae) from the duodenum of Indian skipper frog,  (Amphibia: Dicroglossidae) inhabiting the Western Ghats, India

Two species of digenetic trematodes of the genus Pleurogenoides viz., P. cyanophlyctiShinad & Prasadan (2018a) and P. euphlyctiShinad & Prasadan (2018b) have...

Phylogenetic position of Pleurogenoides species (Plagiorchiida: Pleurogenidae) from the duodenum of Indian skipper frog, Euphlyctis cyanophlyctis (Amphibia: Dicroglossidae) inhabiting the Western Ghats, India

Article Category: Research Note

Published Online: Jan 25, 2020

Page range: 71 - 77

Received: Sep 10, 2019

Accepted: Oct 30, 2019

DOI: https://doi.org/10.2478/helm-2020-0006

Keywords
28S, frogs, India

© 2020 A. Chaudhary, K. Shinad, P. K. Prasadan, H. S. Singh, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Phylogenetic position of Pleurogenoides species (Plagiorchiida: Pleurogenidae) from the duodenum of Indian skipper frog, Euphlyctis cyanophlyctis (Amphibia: Dicroglossidae) inhabiting the Western Ghats, India

Article Category: Research Note

Published Online: Jan 25, 2020

Page range: 71 - 77

Received: Sep 10, 2019

Accepted: Oct 30, 2019

DOI: https://doi.org/10.2478/helm-2020-0006

Keywords28S, frogs, India

© 2020 A. Chaudhary, K. Shinad, P. K. Prasadan, H. S. Singh, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
28S, frogs, India