<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Faecal samples were collected from 224 dogs (47 villages) in Ankara. <italic>Toxocara</italic> spp. eggs were diagnosed in faeces using centrifugal flotation and sedimentation methods. A total of 21 dogs (9.38 %) were positive for <italic>Toxocara</italic> spp. eggs. In this study, we used the PCR technique that, in combination with DNA sequencing, allows the detection and identification of <italic>T.canis</italic> eggs in faeces of infected dogs. For this purpose, the ATPase subunit-6 gene (mtDNA) was selected as a target for the amplification <italic>T. canis</italic>. The primers were used to amplify 217 bp region. Amongst 21 coproscopically detected <italic>Toxocara</italic> isolates from dogs, 5 (23.8 %) samples were PCR-positive for <italic>T. canis</italic>, and the remaining 16 samples were PCR-negative. Results indicate that PCR can detect <italic>Toxocara canis</italic> DNA in faeces of infected dogs, but efficacy was low when compare to sedimentation/flotation. PCR is additional test for diagnosing of this infection. But, the difficulties of identification based on PCR in faecal examinations need to be investigated further.</p></abstract>

Faecal samples were collected from 224 dogs (47 villages) in Ankara. Toxocara spp. eggs were diagnosed in faeces using centrifugal flotation and sedimentation methods. A total of 21 dogs (9.38 %) were positive for Toxocara spp. eggs. In this study, we used the PCR technique that, in combination with DNA sequencing, allows the detection and identification of T.canis eggs in faeces of infected dogs. For this purpose, the ATPase subunit-6 gene (mtDNA) was selected as a target for the amplification T. canis. The primers were used to amplify 217 bp region. Amongst 21 coproscopically detected Toxocara isolates from dogs, 5 (23.8 %) samples were PCR-positive for T. canis, and the remaining 16 samples were PCR-negative. Results indicate that PCR can detect Toxocara canis DNA in faeces of infected dogs, but efficacy was low when compare to sedimentation/flotation. PCR is additional test for diagnosing of this infection. But, the difficulties of identification based on PCR in faecal examinations need to be investigated further.

Detection and identification of <italic>Toxocara canis</italic> in infected dogs using PCR

Faculty of Veterinary Medicine, Department of Parasitology

Helminthologia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Detection and identification of  in infected dogs using PCR

Faecal samples were collected from 224 dogs (47 villages) in Ankara. Toxocara spp. eggs were diagnosed in faeces using centrifugal flotation and sedimentation...

Detection and identification of Toxocara canis in infected dogs using PCR

Published Online: May 04, 2019

Page range: 118 - 123

Received: Sep 26, 2018

Accepted: Dec 18, 2018

DOI: https://doi.org/10.2478/helm-2019-0008

Keywords
dog, faecal examination, ATPase subunit-6

© 2019 H. Öge et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Detection and identification of Toxocara canis in infected dogs using PCR

Published Online: May 04, 2019

Page range: 118 - 123

Received: Sep 26, 2018

Accepted: Dec 18, 2018

DOI: https://doi.org/10.2478/helm-2019-0008

Keywordsdog, faecal examination, ATPase subunit-6

© 2019 H. Öge et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
dog, faecal examination, ATPase subunit-6