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Journals
Gravitational and Space Research
Volume 10 (2022): Issue 1 (January 2022)
Open Access
Polyethersulfone (PES) Membrane on Agar Plates as a Plant Growth Platform for Spaceflight
Alexander Meyers
Alexander Meyers
,
Eric Land
Eric Land
,
Imara Perera
Imara Perera
,
Emma Canaday
Emma Canaday
and
Sarah E. Wyatt
Sarah E. Wyatt
| Dec 31, 2022
Gravitational and Space Research
Volume 10 (2022): Issue 1 (January 2022)
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Article Category:
Research Note
Published Online:
Dec 31, 2022
Page range:
30 - 36
DOI:
https://doi.org/10.2478/gsr-2022-0004
Keywords
Spaceflight
,
advanced plant habitat
,
microgravity
© 2022 Alexander Meyers et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Figure 1
Setup for APEx-07. (A) Plate components from left to right: agar plate, PES membrane, Arabidopsis seed with guar gum, micropore tape. (B) Plates growing vertically in rack.
Figure 2
12-day growth comparison between black and white PES membrane. The more cost-effective white membranes showed comparable growth to black membrane.
Figure 3
Harvest strategy to maintain intact seedlings. (A) Representative 12-day seedling growth in plate, (B) membrane collected with forceps, (C) membrane transferred to lid of Petri dish and wrapped in foil for freezing and shipping, and (D) a frozen plate returned from low Earth orbit.
Figure 4
Ground control Veggie unit at Kennedy Space Center (left) and both Veggie units at capacity aboard the ISS (right).
Success criteria of EVT samples as measured across 60 plates.
Success Criteria
Goal
Observed
Score
Germination
>90%
99%
Excellent
radical emergence
Growth
90%
>99%
Excellent
roots > 4cm
Contamination
<10%
0%
Excellent
Bacterial or fungal growth
Seedling intactness
>90%
100%
Excellent
Dissectible into roots/shoots
RNA quality
>75%
100%
Excellent
RIN> 8, 260/280>2