<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Cell culture on orbit is complicated by numerous operational constraints, including g-loads on the ascent, vibrations, transit time to International Space Station, and delays in experiment initiation. Cryopreserving cells before launch would negate these factors. However, defrosting these cells is problematic, since the traditional method of employing a water bath is not possible. We here describe a unique apparatus designed to accomplish this in a microgravitational environment. This apparatus resulted in rapid defrost of cryopreserved cell cultures and allowed successful tissue culture operations on the station for periods of up to 21 days.</p></abstract>

Cell culture on orbit is complicated by numerous operational constraints, including g-loads on the ascent, vibrations, transit time to International Space Station, and delays in experiment initiation. Cryopreserving cells before launch would negate these factors. However, defrosting these cells is problematic, since the traditional method of employing a water bath is not possible. We here describe a unique apparatus designed to accomplish this in a microgravitational environment. This apparatus resulted in rapid defrost of cryopreserved cell cultures and allowed successful tissue culture operations on the station for periods of up to 21 days.

A Novel Protocol Permitting the Use of Frozen Cell Cultures on Low Earth Orbit

Department of Radiology, Center for Magnetic Resonance Research

Gravitational and Space Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"A Novel Protocol Permitting the Use of Frozen Cell Cultures on Low Earth Orbit"}

Cell culture on orbit is complicated by numerous operational constraints, including g-loads on the ascent, vibrations, transit time to International Space...

A Novel Protocol Permitting the Use of Frozen Cell Cultures on Low Earth Orbit

Article Category: Research Note

Published Online: Jun 14, 2020

Page range: 25 - 30

DOI: https://doi.org/10.2478/gsr-2020-0003

Keywords
Cryopreserved cells, Defrost frozen cultures on orbit, Cell culture on ISS

© 2020 L. S. Kidder et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

A Novel Protocol Permitting the Use of Frozen Cell Cultures on Low Earth Orbit

Article Category: Research Note

Published Online: Jun 14, 2020

Page range: 25 - 30

DOI: https://doi.org/10.2478/gsr-2020-0003

KeywordsCryopreserved cells, Defrost frozen cultures on orbit, Cell culture on ISS

© 2020 L. S. Kidder et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
Cryopreserved cells, Defrost frozen cultures on orbit, Cell culture on ISS