<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The nucleotides are the building blocks of nucleic acids and determining their sequential arrangement had always been an integral part of biological research. Since the past seven decades, researchers from multi-disciplinary fields has been working together to innovate the best sequencing methods. Various methods had been proposed, from some oligonucleotides to the whole genome sequencing, and the growth had gone through adolescence to the mature phase where it is now capable of sequencing the whole genome at a low cost and within a short time frame. DNA sequencing has become a key technology in every discipline of biology and medicine. This review aims to highlight the evolution of DNA sequencing techniques and the machines used, including their principles and key achievements.</p></abstract>

The nucleotides are the building blocks of nucleic acids and determining their sequential arrangement had always been an integral part of biological research. Since the past seven decades, researchers from multi-disciplinary fields has been working together to innovate the best sequencing methods. Various methods had been proposed, from some oligonucleotides to the whole genome sequencing, and the growth had gone through adolescence to the mature phase where it is now capable of sequencing the whole genome at a low cost and within a short time frame. DNA sequencing has become a key technology in every discipline of biology and medicine. This review aims to highlight the evolution of DNA sequencing techniques and the machines used, including their principles and key achievements.

History of DNA Sequencing

Laboratory of Biomedical Microbiology and Immunology

University of Veterinary Medicine and Pharmacy in Košice

Folia Veterinaria

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

{"article-title":"History of DNA Sequencing"}

The nucleotides are the building blocks of nucleic acids and determining their sequential arrangement had always been an integral part of biological research....

History of DNA Sequencing

Published Online: Jul 04, 2020

Page range: 66 - 73

Received: May 05, 2020

Accepted: Jun 06, 2020

DOI: https://doi.org/10.2478/fv-2020-0019

Keywords
DNA, NGS, RNA, sequencing, Sequencing machines

© 2020 P. Tyagi et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

History of DNA Sequencing

Published Online: Jul 04, 2020

Page range: 66 - 73

Received: May 05, 2020

Accepted: Jun 06, 2020

DOI: https://doi.org/10.2478/fv-2020-0019

KeywordsDNA, NGS, RNA, sequencing, Sequencing machines

© 2020 P. Tyagi et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
DNA, NGS, RNA, sequencing, Sequencing machines