<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The investigation was carried out to evaluate the influence of glutathione (GSH: levels 0, 0.1, 0.5 1.0, and 2.0 mM) on the callus growth, shoot multiplication and phytochemicals of <italic>in vitro</italic> shoots of the Date palm cv. Barhee. The optimum concentration of GSH was 1.0 mM. Such concentration improved the callus growth and increased its weight to 312.0 mg, the addition of this substance of the same concentration level showed the highest response rate and the number of shoots per jar (73.34 ± 2.69 and 8.83 ± 0.80 shoots/jar, respectively). Also, the 1.0 GSH application resulted in reducing the percentage of browning to 0.0 compared to the other concentrations. The results of the biochemical analysis revealed that treatments GSH of 0.1, 0.5 and 1.0 mM reduced the total soluble phenols compound (TSPC). These treatments were also more effectively reducing peroxidase (POD) and phenylalanine ammonia-lyase (PAL) activity than the concentrations of 2 mM or 0 (control treatment).</p></abstract>

The investigation was carried out to evaluate the influence of glutathione (GSH: levels 0, 0.1, 0.5 1.0, and 2.0 mM) on the callus growth, shoot multiplication and phytochemicals of in vitro shoots of the Date palm cv. Barhee. The optimum concentration of GSH was 1.0 mM. Such concentration improved the callus growth and increased its weight to 312.0 mg, the addition of this substance of the same concentration level showed the highest response rate and the number of shoots per jar (73.34 ± 2.69 and 8.83 ± 0.80 shoots/jar, respectively). Also, the 1.0 GSH application resulted in reducing the percentage of browning to 0.0 compared to the other concentrations. The results of the biochemical analysis revealed that treatments GSH of 0.1, 0.5 and 1.0 mM reduced the total soluble phenols compound (TSPC). These treatments were also more effectively reducing peroxidase (POD) and phenylalanine ammonia-lyase (PAL) activity than the concentrations of 2 mM or 0 (control treatment).

Effect of glutathione (GSH) on Date palm (<italic>Phoenix dactylifera</italic> L.) micropropagation

Folia Oecologica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Effect of glutathione (GSH) on Date palm ( L.) micropropagation

The investigation was carried out to evaluate the influence of glutathione (GSH: levels 0, 0.1, 0.5 1.0, and 2.0 mM) on the callus growth, shoot multiplication...

Effect of glutathione (GSH) on Date palm (Phoenix dactylifera L.) micropropagation

Published Online: May 13, 2020

Page range: 64 - 69

Received: Feb 06, 2020

Accepted: Apr 05, 2020

DOI: https://doi.org/10.2478/foecol-2020-0008

Keywords
antioxidant substances, browning, phenols compound, phenylalanine ammonia-lyase (PAL), shoot regeneration

© 2020 Ahmed Madi Waheed Al-Mayahi et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Effect of glutathione (GSH) on Date palm (Phoenix dactylifera L.) micropropagation

Published Online: May 13, 2020

Page range: 64 - 69

Received: Feb 06, 2020

Accepted: Apr 05, 2020

DOI: https://doi.org/10.2478/foecol-2020-0008

Keywordsantioxidant substances, browning, phenols compound, phenylalanine ammonia-lyase (PAL), shoot regeneration

© 2020 Ahmed Madi Waheed Al-Mayahi et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
antioxidant substances, browning, phenols compound, phenylalanine ammonia-lyase (PAL), shoot regeneration