<abstract xmlns="http://www.w3.org/1999/xhtml">The influence of basal media composition, concentration of plant growth regulators (PGRs), and the developmental stage of primary explants (dormancy, stage of bud opening and fruit ripening) on the initiation phase of nine <italic>Magnolia</italic> genotypes, including <italic>M. stellata</italic> /Sieb. &amp; Zucc./Maxim., <italic>M.</italic> × <italic>soulangeana</italic> ‘Rustica Rubra’, <italic>M. denudata</italic> Desr., <italic>M.</italic> × <italic>soulangeana</italic> ‘Alexandrina’, <italic>M. liliiflora</italic> Desr., <italic>M. officinalis</italic> var. <italic>biloba</italic> Rehd. &amp; Wils., <italic>M. salicifolia</italic> Maxim., <italic>M.</italic> × <italic>soulangeana</italic> ‘Lennei’, and <italic>M. kobus</italic> DC, was evaluated. The highest efficiency of primary culture initiation of seven <italic>Magnolia</italic> genotypes (except for <italic>M. liliiflora</italic> and <italic>M. salicifolia</italic>) was achieved from primary explants collected in the bud opening stage. A high positive correlation was found between total tannins and efficiency of the primary culture initiation at the fruit ripening stage (r = 0.833). Standardi and Catalano medium (S2) with 0.5 mg l−1 of 6-benzylaminopurine (BAP) was the most appropriate for multiplication of <italic>M</italic>. × <italic>soulangeana</italic> ‘Alexandrina’, whereas tissue cultures of <italic>M</italic>. × <italic>soulangeana</italic> ‘Lennei’ proliferated and grew better on S2 medium with 1.0 mg l−1 of BAP and 1.0 g l−1 of polyvinylpyrrolidone. The requirements for the composition of basal media and concentration of PGRs in the initiation and multiplication stages of micropropagation of various <italic>Magnolia</italic> species and cultivars are genotype-specific.</abstract>

The influence of basal media composition, concentration of plant growth regulators (PGRs), and the developmental stage of primary explants (dormancy, stage of bud opening and fruit ripening) on the initiation phase of nine Magnolia genotypes, including M. stellata /Sieb. & Zucc./Maxim., M. × soulangeana ‘Rustica Rubra’, M. denudata Desr., M. × soulangeana ‘Alexandrina’, M. liliiflora Desr., M. officinalis var. biloba Rehd. & Wils., M. salicifolia Maxim., M. × soulangeana ‘Lennei’, and M. kobus DC, was evaluated. The highest efficiency of primary culture initiation of seven Magnolia genotypes (except for M. liliiflora and M. salicifolia) was achieved from primary explants collected in the bud opening stage. A high positive correlation was found between total tannins and efficiency of the primary culture initiation at the fruit ripening stage (r = 0.833). Standardi and Catalano medium (S2) with 0.5 mg l−1 of 6-benzylaminopurine (BAP) was the most appropriate for multiplication of M. × soulangeana ‘Alexandrina’, whereas tissue cultures of M. × soulangeana ‘Lennei’ proliferated and grew better on S2 medium with 1.0 mg l−1 of BAP and 1.0 g l−1 of polyvinylpyrrolidone. The requirements for the composition of basal media and concentration of PGRs in the initiation and multiplication stages of micropropagation of various Magnolia species and cultivars are genotype-specific.

Genotype-specific requirements for <italic>in vitro</italic> culture initiation and multiplication of <italic>Magnolia</italic> taxa

Mlyňany Arboretum, Institute of Forest Ecology

Department of Botany and Genetics, Faculty of Natural Sciences

University of Constantine the Philosopher

Folia Oecologica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Genotype-specific requirements for  culture initiation and multiplication of  taxa

The influence of basal media composition, concentration of plant growth regulators (PGRs), and the developmental stage of primary explants (dormancy, stage of...

Genotype-specific requirements for in vitro culture initiation and multiplication of Magnolia taxa

Published Online: May 13, 2020

Page range: 34 - 44

Received: Dec 20, 2019

Accepted: Mar 13, 2020

DOI: https://doi.org/10.2478/foecol-2020-0005

Keywords
propagation, multiplication, primary culture initiation

© 2020 Jana Konôpková et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Genotype-specific requirements for in vitro culture initiation and multiplication of Magnolia taxa

Published Online: May 13, 2020

Page range: 34 - 44

Received: Dec 20, 2019

Accepted: Mar 13, 2020

DOI: https://doi.org/10.2478/foecol-2020-0005

Keywordspropagation, multiplication, primary culture initiation

© 2020 Jana Konôpková et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
propagation, multiplication, primary culture initiation