<abstract xmlns="http://www.w3.org/1999/xhtml">This study presents an efficient improvement in the in vitro propagation protocol for one cloned genotype of lentisk (<italic>Pistacia lentiscus</italic> L.) by assessing the effects of gibberellic acid (GA3) concentrations, different cytokinins and amino acids and their concentrations on shoot proliferation, the effects of shoot length on rooting and the effects of compost type (sterile and non-sterile) on acclimatization. The best growth medium for multiple shoot induction was the MS medium supplemented with a combination of 1 mg l−1 BA, 100 mg l−1 tryptophan and 0.5 mg l-1 GA3, which gave a mean shoot length of 1.64 ± 0.07 cm and a mean bud number of 5.46 ± 0.16. The best results in terms of root length, rooting rate and the number of roots per shoot were obtained with 2 cm long shoots. The rooted plantlets were readily acclimatized in the sterile compost. In conclusion, the micropropagation protocol developed in this study can be used for large-scale propagation of <italic>P. lentiscus</italic> L. in reforestation programmes.</abstract>

This study presents an efficient improvement in the in vitro propagation protocol for one cloned genotype of lentisk (Pistacia lentiscus L.) by assessing the effects of gibberellic acid (GA3) concentrations, different cytokinins and amino acids and their concentrations on shoot proliferation, the effects of shoot length on rooting and the effects of compost type (sterile and non-sterile) on acclimatization. The best growth medium for multiple shoot induction was the MS medium supplemented with a combination of 1 mg l−1 BA, 100 mg l−1 tryptophan and 0.5 mg l-1 GA3, which gave a mean shoot length of 1.64 ± 0.07 cm and a mean bud number of 5.46 ± 0.16. The best results in terms of root length, rooting rate and the number of roots per shoot were obtained with 2 cm long shoots. The rooted plantlets were readily acclimatized in the sterile compost. In conclusion, the micropropagation protocol developed in this study can be used for large-scale propagation of P. lentiscus L. in reforestation programmes.

An improved micropropagation protocol for lentisk (<italic>Pistacia lentiscus</italic> L.)

Agriculture Faculty, Horticulture Department

Folia Horticulturae

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

An improved micropropagation protocol for lentisk ( L.)

This study presents an efficient improvement in the in vitro propagation protocol for one cloned genotype of lentisk (Pistacia lentiscus L.) by assessing the...

An improved micropropagation protocol for lentisk (Pistacia lentiscus L.)

Published Online: Jun 30, 2019

Page range: 61 - 69

Received: Jul 09, 2018

Accepted: Dec 28, 2018

DOI: https://doi.org/10.2478/fhort-2019-0003

Keywords
acclimatization, amino acid, cytokinins, gibberellic acid, rooting rate, shoot proliferation

© 2019 Hakan Yildirim et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

An improved micropropagation protocol for lentisk (Pistacia lentiscus L.)

Published Online: Jun 30, 2019

Page range: 61 - 69

Received: Jul 09, 2018

Accepted: Dec 28, 2018

DOI: https://doi.org/10.2478/fhort-2019-0003

Keywordsacclimatization, amino acid, cytokinins, gibberellic acid, rooting rate, shoot proliferation

© 2019 Hakan Yildirim et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
acclimatization, amino acid, cytokinins, gibberellic acid, rooting rate, shoot proliferation