<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Gladiolus is one of the most important lucrative cut flower crops that is commercially cultivated worldwide due to its various spike forms, size, and shape and color combinations. In order to further increase the commercial and horticultural value by improving the ornamental traits of gladiolus ‘White Prosperity’, polyploidy was induced by soaking gladiolus corms in different colchicine concentrations (0.1%, 0.2% and 0.3%) for 24 h. Different colchicine concentrations had a little effect on sprouting and survival percentage but it significantly delayed the emergence of sprouts. About one third decreases in plant height along with reduction in number of leaves per plant, leaf area, length and width, chlorophyll content, diameter and number of cormlets per corm was observed in treated plants. Colchicine at 0.1% concentration improved the ornamental value of gladiolus by increasing vase life whereas colchicine at 0.3% was effective in increasing floret diameter. However, the colchicine treated plants exhibited delayed and reduced percentage of flowering corms. Pollen and stomatal study was done for the identification of polyploidy and it showed that both pollen and stomata size were increased while stomatal density and pollen fertility was significantly reduced in polyploid plants. Induction of polyploidy (mixoploids + octoploids) was achieved in all concentrations, however 0.2% and 0.3% concentrations of colchicine were effective for producing large number of polyploid plants. But at 0.1% concentration of colchicine, majority of plants did not show any change in their original ploidy level (tetraploid). These putative polyploids may be helpful for further improvement in ornamental and horticultural value of gladiolus.</p></abstract>

Gladiolus is one of the most important lucrative cut flower crops that is commercially cultivated worldwide due to its various spike forms, size, and shape and color combinations. In order to further increase the commercial and horticultural value by improving the ornamental traits of gladiolus ‘White Prosperity’, polyploidy was induced by soaking gladiolus corms in different colchicine concentrations (0.1%, 0.2% and 0.3%) for 24 h. Different colchicine concentrations had a little effect on sprouting and survival percentage but it significantly delayed the emergence of sprouts. About one third decreases in plant height along with reduction in number of leaves per plant, leaf area, length and width, chlorophyll content, diameter and number of cormlets per corm was observed in treated plants. Colchicine at 0.1% concentration improved the ornamental value of gladiolus by increasing vase life whereas colchicine at 0.3% was effective in increasing floret diameter. However, the colchicine treated plants exhibited delayed and reduced percentage of flowering corms. Pollen and stomatal study was done for the identification of polyploidy and it showed that both pollen and stomata size were increased while stomatal density and pollen fertility was significantly reduced in polyploid plants. Induction of polyploidy (mixoploids + octoploids) was achieved in all concentrations, however 0.2% and 0.3% concentrations of colchicine were effective for producing large number of polyploid plants. But at 0.1% concentration of colchicine, majority of plants did not show any change in their original ploidy level (tetraploid). These putative polyploids may be helpful for further improvement in ornamental and horticultural value of gladiolus.

Induction and identification of colchicine induced polyploidy in <italic>Gladiolus grandiflorus</italic> ‘White Prosperity’

Department of Horticulture PMAS Arid Agriculture

Department of Plant Breeding and Genetics PMAS Arid Agriculture

Folia Horticulturae

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Induction and identification of colchicine induced polyploidy in  ‘White Prosperity’

Gladiolus is one of the most important lucrative cut flower crops that is commercially cultivated worldwide due to its various spike forms, size, and shape and...

Induction and identification of colchicine induced polyploidy in Gladiolus grandiflorus ‘White Prosperity’

Published Online: Dec 14, 2018

Page range: 307 - 319

Received: Nov 11, 2017

Accepted: May 31, 2018

DOI: https://doi.org/10.2478/fhort-2018-0026

Keywords
chromosome, diploid, pollen, stomata, tetraploid

© 2018 2018 Ayesha Manzoor et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Induction and identification of colchicine induced polyploidy in Gladiolus grandiflorus ‘White Prosperity’

Published Online: Dec 14, 2018

Page range: 307 - 319

Received: Nov 11, 2017

Accepted: May 31, 2018

DOI: https://doi.org/10.2478/fhort-2018-0026

Keywordschromosome, diploid, pollen, stomata, tetraploid

© 2018 2018 Ayesha Manzoor et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
chromosome, diploid, pollen, stomata, tetraploid