<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Conservation of bamboos for future exploitation as fuel, fibre and as an ingredient for cosmetics depends on knowledge of its natural genetic variation. The study of molecular genetic diversity in bamboos will provide important information for its conservation. This article reports on the genetic diversity in 25 species representing five genera of bamboos found in Indonesia using Random Amplified Polymorphic DNA (RAPD) molecular markers. Out of 40 primers, 24 primers produced 1107 total bands and 86.21% of polymorphic bands across the 25 species. Sixteen bands were uniquely found in one species only and their presence or absence helped to define nine bamboo species. RAPD band sizes ranged from 162 to 2247 base pairs. A dendrogram based on the similarity coefficient of Dice divided the bamboo species into three big clusters. In conclusion, RAPD can capture the diversity among five different bamboo genera and has a great potential to be used in the study of genetic diversity in Indonesian bamboos.</p></abstract>

Conservation of bamboos for future exploitation as fuel, fibre and as an ingredient for cosmetics depends on knowledge of its natural genetic variation. The study of molecular genetic diversity in bamboos will provide important information for its conservation. This article reports on the genetic diversity in 25 species representing five genera of bamboos found in Indonesia using Random Amplified Polymorphic DNA (RAPD) molecular markers. Out of 40 primers, 24 primers produced 1107 total bands and 86.21% of polymorphic bands across the 25 species. Sixteen bands were uniquely found in one species only and their presence or absence helped to define nine bamboo species. RAPD band sizes ranged from 162 to 2247 base pairs. A dendrogram based on the similarity coefficient of Dice divided the bamboo species into three big clusters. In conclusion, RAPD can capture the diversity among five different bamboo genera and has a great potential to be used in the study of genetic diversity in Indonesian bamboos.

Evaluation of RAPD markers for molecular identification of five bamboo genera from Indonesia

Faculty of Mathematics and Natural Sciences, Department of Biology

Folia Forestalia Polonica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Conservation of bamboos for future exploitation as fuel, fibre and as an ingredient for cosmetics depends on knowledge of its natural genetic variation. The...

Evaluation of RAPD markers for molecular identification of five bamboo genera from Indonesia

Published Online: Dec 30, 2019

Page range: 255 - 266

Received: Mar 05, 2019

Accepted: Aug 05, 2019

DOI: https://doi.org/10.2478/ffp-2019-0025

Keywords
bamboo, genetics diversity, RAPD, variation

© 2019 Annisa et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Evaluation of RAPD markers for molecular identification of five bamboo genera from Indonesia

Published Online: Dec 30, 2019

Page range: 255 - 266

Received: Mar 05, 2019

Accepted: Aug 05, 2019

DOI: https://doi.org/10.2478/ffp-2019-0025

Keywordsbamboo, genetics diversity, RAPD, variation

© 2019 Annisa et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
bamboo, genetics diversity, RAPD, variation