<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The aim of the study was to determine the hybridity of the F<sub>1</sub> generation of <italic>Vaccinium cf. uliginosum</italic> × <italic>V. vitis-idaea</italic> × <italic>Oxycoccus macrocarpus</italic> created through the consecutive crossing of some common berry species of the family Ericaceae (bog whortleberry, cowberry and marsh cranberry) at the tetraploid level. Certain aspects of phenology and morphometric parameters of vegetative organs of the supposed hybrid and parent plants were analysed using traditional methods of comparative analysis. Molecular genetic assay, including random amplification of polymorphic DNA, simple sequence repeat and sequencing, were also used. Comparison of the phenological and morphometric features of the experimental plant and its parents allows suggesting that in the combination of crossing of (<italic>V. uliginosum</italic> × <italic>V. vitis-idaea</italic>) × <italic>O. macrocarpus</italic> (the cultivar Searles), a three-species hybrid was created. The allelic variants, specific for <italic>V. uliginosum, V. vitis-idaea</italic> and <italic>O. macrocarpus</italic>, were detected in the <italic>V. cf. uliginosum</italic> × <italic>V. vitis-idaea</italic> × <italic>O. macrocarpus</italic> genotype. A next-generation sequencing approach is suggested for estimating the share of the genomes of <italic>Vaccinium</italic> spp. in the formation of the interspecies hybrid.</p></abstract>

The aim of the study was to determine the hybridity of the F1 generation of Vaccinium cf. uliginosum × V. vitis-idaea × Oxycoccus macrocarpus created through the consecutive crossing of some common berry species of the family Ericaceae (bog whortleberry, cowberry and marsh cranberry) at the tetraploid level. Certain aspects of phenology and morphometric parameters of vegetative organs of the supposed hybrid and parent plants were analysed using traditional methods of comparative analysis. Molecular genetic assay, including random amplification of polymorphic DNA, simple sequence repeat and sequencing, were also used. Comparison of the phenological and morphometric features of the experimental plant and its parents allows suggesting that in the combination of crossing of (V. uliginosum × V. vitis-idaea) × O. macrocarpus (the cultivar Searles), a three-species hybrid was created. The allelic variants, specific for V. uliginosum, V. vitis-idaea and O. macrocarpus, were detected in the V. cf. uliginosum × V. vitis-idaea × O. macrocarpus genotype. A next-generation sequencing approach is suggested for estimating the share of the genomes of Vaccinium spp. in the formation of the interspecies hybrid.

Hybridity of a plant created in a combination of crossing of (<italic>Vaccinium uliginosum</italic> L. × <italic>V. vitis-idaea</italic> L.) × <italic>Oxycoccus macrocarpus</italic> (Aiton) Pursh at the tetraploid level

Forest Research Institute of the National Academy of Sciences of Belarus

Folia Forestalia Polonica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Hybridity of a plant created in a combination of crossing of ( L. ×  L.) ×  (Aiton) Pursh at the tetraploid level

The aim of the study was to determine the hybridity of the F1 generation of Vaccinium cf. uliginosum × V. vitis-idaea × Oxycoccus macrocarpus created through...

Hybridity of a plant created in a combination of crossing of (Vaccinium uliginosum L. × V. vitis-idaea L.) × Oxycoccus macrocarpus (Aiton) Pursh at the tetraploid level

Published Online: Jan 25, 2019

Page range: 281 - 291

Received: Jul 25, 2018

Accepted: Oct 01, 2018

DOI: https://doi.org/10.2478/ffp-2018-0029

Keywords
consecutive crossing, DNA markers, Ericaceae, hybridisation, sequencing, tetraploids

© 2018 Aleh Marozau et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Hybridity of a plant created in a combination of crossing of (Vaccinium uliginosum L. × V. vitis-idaea L.) × Oxycoccus macrocarpus (Aiton) Pursh at the tetraploid level

Published Online: Jan 25, 2019

Page range: 281 - 291

Received: Jul 25, 2018

Accepted: Oct 01, 2018

DOI: https://doi.org/10.2478/ffp-2018-0029

Keywordsconsecutive crossing, DNA markers, Ericaceae, hybridisation, sequencing, tetraploids

© 2018 Aleh Marozau et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
consecutive crossing, DNA markers, Ericaceae, hybridisation, sequencing, tetraploids