<abstract xmlns="http://www.w3.org/1999/xhtml">This study aimed to investigate the effects of possible zinc (Zn) and molybdenum (Mo) contaminations on the critically endangered European Bluestar (<italic>Amsonia orientalis</italic>). The effects of Zn and Mo were tested in a dose-dependent manner on <italic>in vitro</italic> cultures. Zn at 0.1 mM in the medium inhibited root development whereas Mo showed the same effect only at ≥2.5 mM concentration. Gradual inhibition of shoot development was observed after treatment with both metals. Protein contents were also negatively affected by increasing metal concentrations, while proline levels increased gradually. Successive increases in metal concentrations resulted in higher hydrogen peroxide (H2O2) and malondialdehyde (MDA) concentrations. The activity of the antioxidant enzymes, peroxidase (POD) and catalase (CAT), were found to be enhanced in response to increasing metal concentrations. Superoxide dismutase (SOD) activity decreased after Zn treatment but increased after Mo treatment. A marked increase in POD and CAT in response to metal stress suggests that these enzymes might have a significant cooperative role in regulating H2O2 production, although CAT, in response to drought and salt stress, has been reported to only play a supplementary role in <italic>A. orientalis</italic>. These results indicated that <italic>A. orientalis</italic> is susceptible to long-term Zn stress but can tolerate up to 2.5 mM Mo in the long-term. Deficiency of Mo is more common than high toxic concentrations in the environment. Therefore Zn contamination should be considered as one of the major threats for <italic>A. orientalis</italic> in its native habitat.</abstract>

This study aimed to investigate the effects of possible zinc (Zn) and molybdenum (Mo) contaminations on the critically endangered European Bluestar (Amsonia orientalis). The effects of Zn and Mo were tested in a dose-dependent manner on in vitro cultures. Zn at 0.1 mM in the medium inhibited root development whereas Mo showed the same effect only at ≥2.5 mM concentration. Gradual inhibition of shoot development was observed after treatment with both metals. Protein contents were also negatively affected by increasing metal concentrations, while proline levels increased gradually. Successive increases in metal concentrations resulted in higher hydrogen peroxide (H2O2) and malondialdehyde (MDA) concentrations. The activity of the antioxidant enzymes, peroxidase (POD) and catalase (CAT), were found to be enhanced in response to increasing metal concentrations. Superoxide dismutase (SOD) activity decreased after Zn treatment but increased after Mo treatment. A marked increase in POD and CAT in response to metal stress suggests that these enzymes might have a significant cooperative role in regulating H2O2 production, although CAT, in response to drought and salt stress, has been reported to only play a supplementary role in A. orientalis. These results indicated that A. orientalis is susceptible to long-term Zn stress but can tolerate up to 2.5 mM Mo in the long-term. Deficiency of Mo is more common than high toxic concentrations in the environment. Therefore Zn contamination should be considered as one of the major threats for A. orientalis in its native habitat.

Effects of zinc and molybdenum on European Bluestar (<italic>Amsonia orientalis</italic>): An <italic>in vitro</italic> study

The EuroBiotech Journal

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Effects of zinc and molybdenum on European Bluestar (): An  study

This study aimed to investigate the effects of possible zinc (Zn) and molybdenum (Mo) contaminations on the critically endangered European Bluestar (Amsonia...

Effects of zinc and molybdenum on European Bluestar (Amsonia orientalis): An in vitro study

Article Category: Research Article

Published Online: Jan 18, 2020

Page range: 32 - 41

DOI: https://doi.org/10.2478/ebtj-2020-0005

Keywords
Molybdenum, oxidative stress, tissue culture, zinc

© 2020 Arda Acemi, Yonca Avcı Duman, Yonca Yüzügüllü Karakuş and Fazıl Özen, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Effects of zinc and molybdenum on European Bluestar (Amsonia orientalis): An in vitro study

Article Category: Research Article

Published Online: Jan 18, 2020

Page range: 32 - 41

DOI: https://doi.org/10.2478/ebtj-2020-0005

KeywordsMolybdenum, oxidative stress, tissue culture, zinc

© 2020 Arda Acemi, Yonca Avcı Duman, Yonca Yüzügüllü Karakuş and Fazıl Özen, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
Molybdenum, oxidative stress, tissue culture, zinc