<abstract xmlns="http://www.w3.org/1999/xhtml"><p>There is a growing need for new formulations of carriers with better protection for bacterial inoculum. One of the newer techniques in inoculum making is encapsulation method. With this method, the whole bacterial cells are immobilized in defined space – matrix, where the cells are protected from environmental activities before use. Encapsulation of the inoculum was performed with ionic gelation method. The alginate-based microparticles (500-600 µm) containing viable B. japonicum strain were solidified in CaCl<sub>2</sub>. The initial number of viable bacteria in every sample was 9.0 log CFU/ml. Chitosan coated particles had a higher mortality rate than non-coated particles, with 1.3 log CFU/ml in lyophilized and wet microparticles stored at room temperature. High viability of B. japonicum was registered in wet particles stored at constant −20°C for thirty days with a viability rate of 8.84 log CFU/ml.</p></abstract>

There is a growing need for new formulations of carriers with better protection for bacterial inoculum. One of the newer techniques in inoculum making is encapsulation method. With this method, the whole bacterial cells are immobilized in defined space – matrix, where the cells are protected from environmental activities before use. Encapsulation of the inoculum was performed with ionic gelation method. The alginate-based microparticles (500-600 µm) containing viable B. japonicum strain were solidified in CaCl2. The initial number of viable bacteria in every sample was 9.0 log CFU/ml. Chitosan coated particles had a higher mortality rate than non-coated particles, with 1.3 log CFU/ml in lyophilized and wet microparticles stored at room temperature. High viability of B. japonicum was registered in wet particles stored at constant −20°C for thirty days with a viability rate of 8.84 log CFU/ml.

The Storage Efficiency of Immobilized <italic>Bradyrhizobium japonicum</italic> Strain Using Encapsulation Method

Faculty of Agriculture, Department of Microbiology

Faculty of Agriculture, Department of Chemistry

Contemporary Agriculture

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The Storage Efficiency of Immobilized  Strain Using Encapsulation Method

There is a growing need for new formulations of carriers with better protection for bacterial inoculum. One of the newer techniques in inoculum making is...

The Storage Efficiency of Immobilized Bradyrhizobium japonicum Strain Using Encapsulation Method

Published Online: Jul 30, 2019

Page range: 43 - 50

Received: Dec 03, 2018

Accepted: Feb 28, 2019

DOI: https://doi.org/10.2478/contagri-2019-0008

Keywords
Encapsulation, Bradyrhizobium japonicum, storage, viability, biofertilizers

© 2019 Dora Krznarić et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The Storage Efficiency of Immobilized Bradyrhizobium japonicum Strain Using Encapsulation Method

Published Online: Jul 30, 2019

Page range: 43 - 50

Received: Dec 03, 2018

Accepted: Feb 28, 2019

DOI: https://doi.org/10.2478/contagri-2019-0008

KeywordsEncapsulation, Bradyrhizobium japonicum, storage, viability, biofertilizers

© 2019 Dora Krznarić et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
Encapsulation, Bradyrhizobium japonicum, storage, viability, biofertilizers