<abstract xmlns="http://www.w3.org/1999/xhtml">Efficacy of ethanol and methanol extracts of medicinal herb <italic>Withania somnifera</italic> roots was evaluated in Nile tilapia <italic>Oreochromis niloticus</italic> on immunostimulation and growth. <italic>Withania</italic> roots were extracted with water, ethanol, methanol, methylene chloride, hexane, successive methanol. Phenol, flavonoid content and antiradical activity of all the extracts were measured. Ethanol extract showed the highest phenol, flavonoid content and antiradical activity followed by methanol extract. Subsequently, Nile tilapia (n = 126) were fed diets containing different concentrations (0.0, 0.3, 0.5 and 0.7 g kg−1 feed) of ethanol and methanol extracts of <italic>W. somnifera</italic> roots for 30 days (3 replicates). Fish fed plant extract fortified diets showed significantly better (<italic>P&lt;0.05</italic>) immunological, haematological, biochemical and growth parameters compared to the fish fed control diet. Fish fed diet containing ethanol extract at the concentration of 0.7 g kg−1 feed showed the highest immunological (phagocytotic activity, respiratory burst activity, serum lysozyme, total protein, total immunoglobulin), haematological (total red blood cells, haemoglobin, hematocrit, total white blood cells, lymphocyte), biochemical (reduced glutathione, glutathione reductase activity) and growth (final weight, weight gain, daily weight gain, specific growth rate) parameters. The plant extract might act as potent free radical scavenger in fish tissues and have tissue protecting ability, thus increasing fish health.</abstract>

Efficacy of ethanol and methanol extracts of medicinal herb Withania somnifera roots was evaluated in Nile tilapia Oreochromis niloticus on immunostimulation and growth. Withania roots were extracted with water, ethanol, methanol, methylene chloride, hexane, successive methanol. Phenol, flavonoid content and antiradical activity of all the extracts were measured. Ethanol extract showed the highest phenol, flavonoid content and antiradical activity followed by methanol extract. Subsequently, Nile tilapia (n = 126) were fed diets containing different concentrations (0.0, 0.3, 0.5 and 0.7 g kg−1 feed) of ethanol and methanol extracts of W. somnifera roots for 30 days (3 replicates). Fish fed plant extract fortified diets showed significantly better (P<0.05) immunological, haematological, biochemical and growth parameters compared to the fish fed control diet. Fish fed diet containing ethanol extract at the concentration of 0.7 g kg−1 feed showed the highest immunological (phagocytotic activity, respiratory burst activity, serum lysozyme, total protein, total immunoglobulin), haematological (total red blood cells, haemoglobin, hematocrit, total white blood cells, lymphocyte), biochemical (reduced glutathione, glutathione reductase activity) and growth (final weight, weight gain, daily weight gain, specific growth rate) parameters. The plant extract might act as potent free radical scavenger in fish tissues and have tissue protecting ability, thus increasing fish health.

Dietary Administration of Ethanol and Methanol Extracts of <italic>Withania somnifera</italic> Root Stimulates Innate Immunity, Physiological Parameters and Growth in Nile Tilapia <italic>Oreochromis niloticus</italic>

Fish Endocrinology Research Unit, Department of Zoology

Croatian Journal of Fisheries

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Dietary Administration of Ethanol and Methanol Extracts of  Root Stimulates Innate Immunity, Physiological Parameters and Growth in Nile Tilapia 

Efficacy of ethanol and methanol extracts of medicinal herb Withania somnifera roots was evaluated in Nile tilapia Oreochromis niloticus on immunostimulation...

Dietary Administration of Ethanol and Methanol Extracts of Withania somnifera Root Stimulates Innate Immunity, Physiological Parameters and Growth in Nile Tilapia Oreochromis niloticus

Published Online: Oct 18, 2019

Page range: 107 - 118

Received: Aug 24, 2018

Accepted: Mar 20, 2019

DOI: https://doi.org/10.2478/cjf-2019-0012

Keywords
Ethanol extract, Methanol extract, Immunostimulation, Growth, Antioxidant

© 2019 Debosree Mukherjee et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Dietary Administration of Ethanol and Methanol Extracts of Withania somnifera Root Stimulates Innate Immunity, Physiological Parameters and Growth in Nile Tilapia Oreochromis niloticus

Published Online: Oct 18, 2019

Page range: 107 - 118

Received: Aug 24, 2018

Accepted: Mar 20, 2019

DOI: https://doi.org/10.2478/cjf-2019-0012

KeywordsEthanol extract, Methanol extract, Immunostimulation, Growth, Antioxidant

© 2019 Debosree Mukherjee et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
Ethanol extract, Methanol extract, Immunostimulation, Growth, Antioxidant