<abstract xmlns="http://www.w3.org/1999/xhtml">

The aim of the present study was to evaluate the hypoglycemic activity of the aqueous extract from the fruit walls of <italic>Phaseolus vulgaris</italic> pods and to examine the potential mechanism underlying the improvement of the glycemic level. In the course of the study, diabetes mellitus was induced in rats with a single intraperitoneal injection of streptozotocin (45 mg·kg−1 b.w.). Diabetic and control rats were then orally administered with a single-dose or repeated-dose (28 day) of <italic>P. vulgaris</italic> extract (200 mg·kg−1). Results show that the extract was found to possess significant hypoglycemic activity, and the study of glucose utilization by isolated rat hemidiaphragm suggests that the aqueous extract may enhance the peripheral utilization of glucose. The subsequent experiments have revealed that the <italic>P. vulgaris</italic> extract could increase glucose transporter 4 (GLUT-4) content in skeletal muscle cells of control and diabetic rats. Our data also indicate that the <italic>P. vulgaris</italic> extract did not affect the content of the insulin receptor, but significantly reduced the total tyrosine kinase activity in skeletal muscle cells of both experimental groups of rats. The present results clearly indicated that <italic>P. vulgaris</italic> extract may be beneficial for reducing hyperglycemia through its potency in regulation of glucose utilization via GLUT-4, but the current mechanism remains to be unidentified.
</abstract>

The aim of the present study was to evaluate the hypoglycemic activity of the aqueous extract from the fruit walls of Phaseolus vulgaris pods and to examine the potential mechanism underlying the improvement of the glycemic level. In the course of the study, diabetes mellitus was induced in rats with a single intraperitoneal injection of streptozotocin (45 mg·kg−1 b.w.). Diabetic and control rats were then orally administered with a single-dose or repeated-dose (28 day) of P. vulgaris extract (200 mg·kg−1). Results show that the extract was found to possess significant hypoglycemic activity, and the study of glucose utilization by isolated rat hemidiaphragm suggests that the aqueous extract may enhance the peripheral utilization of glucose. The subsequent experiments have revealed that the P. vulgaris extract could increase glucose transporter 4 (GLUT-4) content in skeletal muscle cells of control and diabetic rats. Our data also indicate that the P. vulgaris extract did not affect the content of the insulin receptor, but significantly reduced the total tyrosine kinase activity in skeletal muscle cells of both experimental groups of rats. The present results clearly indicated that P. vulgaris extract may be beneficial for reducing hyperglycemia through its potency in regulation of glucose utilization via GLUT-4, but the current mechanism remains to be unidentified.

Hypoglycemic activity of <italic>Phaseolus vulgaris</italic> (L.) aqueous extract in type 1 diabetic rats

Department of Biochemistry Educational an d Scientific Center “Institute of Biology and Medicine”

Taras Shevchenko National University of Kyiv

Department of Cytology Educational and Scientific Center “Institute of Biology and Medicine”

Current Issues in Pharmacy and Medical Sciences

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Hypoglycemic activity of  (L.) aqueous extract in type 1 diabetic rats

The aim of the present study was to evaluate the hypoglycemic activity of the aqueous extract from the fruit walls of Phaseolus vulgaris pods and to examine...

Hypoglycemic activity of Phaseolus vulgaris (L.) aqueous extract in type 1 diabetic rats

Published Online: Dec 31, 2019

Page range: 210 - 218

Received: Feb 19, 2019

Accepted: Apr 24, 2019

DOI: https://doi.org/10.2478/cipms-2019-0036

Keywords
aqueous extract, hypoglycemic activity, diabetes mellitus

© 2019 Tetiana Halenova et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Hypoglycemic activity of Phaseolus vulgaris (L.) aqueous extract in type 1 diabetic rats

Published Online: Dec 31, 2019

Page range: 210 - 218

Received: Feb 19, 2019

Accepted: Apr 24, 2019

DOI: https://doi.org/10.2478/cipms-2019-0036

Keywordsaqueous extract, hypoglycemic activity, diabetes mellitus

© 2019 Tetiana Halenova et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
aqueous extract, hypoglycemic activity, diabetes mellitus