<abstract xmlns="http://www.w3.org/1999/xhtml"><p> The aim of the study was to verify the thesis that the cardiotoxic effects of doxorubicin are connected with activation of the ubiquitin - proteasome pathway followed by protein degradation. The expression of myocardial damage markers - fatty acid binding protein (H-FABP) and brain natriuretic peptide (BNP) was evaluated in rat fetal cardiomyocytes simultaneously treated with doxorubicin and the proteasome inhibitor - bortezomib. The level of H-FABP and BNP protein under the influence of doxorubicin was decreased below the detection threshold with unchanged (H-FABP) or elevated (BNP) mRNA expression level. Against the expectations, the inhibitor of proteasome did not abolish this effect. The observed abnormal expression of BNP and H-FABP protein after doxorubicin treatment makes their diagnostic significance in anthracycline cardiotoxicity questionable.</p></abstract>

The aim of the study was to verify the thesis that the cardiotoxic effects of doxorubicin are connected with activation of the ubiquitin - proteasome pathway followed by protein degradation. The expression of myocardial damage markers - fatty acid binding protein (H-FABP) and brain natriuretic peptide (BNP) was evaluated in rat fetal cardiomyocytes simultaneously treated with doxorubicin and the proteasome inhibitor - bortezomib. The level of H-FABP and BNP protein under the influence of doxorubicin was decreased below the detection threshold with unchanged (H-FABP) or elevated (BNP) mRNA expression level. Against the expectations, the inhibitor of proteasome did not abolish this effect. The observed abnormal expression of BNP and H-FABP protein after doxorubicin treatment makes their diagnostic significance in anthracycline cardiotoxicity questionable.

The influence of proteasome inhibitor on the expression of cardiomyocytes damage markers after incubation with doxorubicin

Independent Medical Biology Unit, Medical University of Lublin, Poland

Department of Histology and Embryology, Medical University of Lublin, Poland

Department of Pneumology Oncology and Allergology, Medical University of Lublin, Poland

IMMUNIQ, Beata Solon-Gogol, Sasiedzka 1, 44-240 Zory, Poland

Regional Dental Clinic Independent Public Health Care, Lubartowska 58, Lublin, Poland

Department of Pediatric Pulmonology and Rheumatology, Medical University of Lublin, Poland

Current Issues in Pharmacy and Medical Sciences

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The aim of the study was to verify the thesis that the cardiotoxic effects of doxorubicin are connected with activation of the ubiquitin - proteasome pathway...

The influence of proteasome inhibitor on the expression of cardiomyocytes damage markers after incubation with doxorubicin

Published Online: Nov 25, 2014

Page range: 88 - 91

Received: Jun 11, 2014

Accepted: Jun 13, 2014

DOI: https://doi.org/10.2478/cipms-2014-0020

Keywords
doxorubicin, BNP, H-FABP, proteasom

© 2014

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The influence of proteasome inhibitor on the expression of cardiomyocytes damage markers after incubation with doxorubicin

Published Online: Nov 25, 2014

Page range: 88 - 91

Received: Jun 11, 2014

Accepted: Jun 13, 2014

DOI: https://doi.org/10.2478/cipms-2014-0020

Keywordsdoxorubicin, BNP, H-FABP, proteasom

© 2014

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
doxorubicin, BNP, H-FABP, proteasom