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Cytotoxic effects of the synthetic oestrogens and androgens on Balb/c 3T3 and HepG2 cells


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1. Amacher D.E.: Drug-associated mitochondrial toxicity and its detection. Curr Med Chem 2005, 12, 1829-1839.10.2174/0929867054546663 Search in Google Scholar

2. Boettcher M., Kosmehl T., Braunbeck T.: Low-dose effects and biphasic effect profiles: is trenbolone a genotoxicant? Mut Res 2011, 723, 152-157.10.1016/j.mrgentox.2011.04.012 Search in Google Scholar

3. Borenfreund E., Puerner J.A.: Toxicity determined in vitro by morphological alterations and neutral red absorption. Toxicol Lett 1985, 24, 119-124.10.1016/0378-4274(85)90046-3 Search in Google Scholar

4. Bradford M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein using the principle of dye binding. Anal Biochem 1976, 72, 248-254.10.1016/0003-2697(76)90527-3 Search in Google Scholar

5. Caraci F., Pistara V., Corsaro A., Tomasello F., Giuffrida M.L., Soetino M.A., Nicoletti F.: Neurotoxic properties of the anabolic androgenic steroids nandrolone and methandrostenolone in primary neuronal cultures. J Neurosci Res 2011, 89, 592-600.10.1002/jnr.22578 Search in Google Scholar

6. Dorn S.B., Bolt H.M., Thevis M., Diel P., Degen G.H.: Induction of micronuclei in V79 cells by the anabolic doping steroids tetrahydrogestrinone and trenbolone. Arch Toxicol 2008, 82, 257-263.10.1007/s00204-007-0241-2 Search in Google Scholar

7. Eisenbrand G., Pool-Zobel B., Baker V., Balls M., Blaauboer B.J., Boobis A., Carere A., Kevekordes S., Lhuguenot J.C., Pieters R., Kleiner J.: Methods of in vitro toxicology. Food Chem Toxicol 2002, 40, 193-236.10.1016/S0278-6915(01)00118-1 Search in Google Scholar

8. Felty Q., Roy D.: Estrogen, mitochondria, and growth of cancer and non-cancer cells. J Carcinog 2005, 4, 1-18. Search in Google Scholar

9. Fotakis G., Timbrell J.A.: In vitro cytotoxicity assays: comparison of LDH, neutral red, MTT and protein assay in hepatoma cell lines following exposure to cadmium chloride. Toxicol Lett 2006, 160, 171-177.10.1016/j.toxlet.2005.07.00116111842 Search in Google Scholar

10. Giannitrapani L., Soresi M., La Spada E., Cervello M., D’Alessandro N., Montalto G.: Sex hormones and risk of liver tumor. Ann NY Acad Sci 2006, 1089, 228-236.10.1196/annals.1386.04417261770 Search in Google Scholar

11. Gerets H.H.J., Hanon E., Cornet M., Dhalluin S., Depelchin O., Canning M., Atienzar F.A.: Selection of cytotoxicity markers for the screening of new chemical entities in a pharmaceutical context: a preliminary study using a multiplexing approach. Toxicol in Vitro 2009, 23, 319-332.10.1016/j.tiv.2008.11.01219110050 Search in Google Scholar

12. Ivanova L., Uhlig S.: A bioassay for the simultaneous measurement of metabolic activity, membrane integrity, and lysosomal activity in cell cultures. Anal Biochem 2008, 379, 16-19.10.1016/j.ab.2008.04.035 Search in Google Scholar

13. Iwasawa A., Ayaki M., Niwano Y.: Cell viability score (CVS) as a good indicator of critical concentration of benzalkonium chloride for toxicity in cultured ocular surface cell lines. Regul Toxicol Pharmacol 2013, 66, 177-183.10.1016/j.yrtph.2013.03.014 Search in Google Scholar

14. Konac E., Ekmekci A., Barkar V., Yilmaz A., Erbas D.: Effects of diethylstilbestrol in human lymphocytes in vitro: a dose and time-dependent study on genotoxic, cytotoxic and apoptotic effects. Mol Cell Biochem 2005, 276, 45-53.10.1007/s11010-005-2815-8 Search in Google Scholar

15. Korzeniewski C., Calleawert D.M.: An enzyme-release assay for natural cytotoxicity. J Immunol Methods 1983, 64, 313-320.10.1016/0022-1759(83)90438-6 Search in Google Scholar

16. Maier W.E., Herman J.R.: Pharmacology and toxicology of ethinyl estradiol and norethindrone acetate in experimental animals. Regul Toxicol Pharmacol 2001, 34, 53-61.10.1006/rtph.2001.1483 Search in Google Scholar

17. Mosmann T.: Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assay. J Immunol Methods 1983, 65, 55-63.10.1016/0022-1759(83)90303-4 Search in Google Scholar

18. Oda T., Tanaka M., Sasaki T.: Cytotoxicity of synthetic estrogen and related compounds in various tumor-derived cells. Biol Pharm Bull 2001, 24, 1142-1144.10.1248/bpb.24.114211642319 Search in Google Scholar

19. Orlando R., Caruso A., Molinaro G., Motolese M., Matrisciano F., Togna G., Melchiorri D., Nicoletti F., Bruno V.: Nanomolar concentrations of anabolic-androgenic steroids amplify excitotoxic neuronal death in mixed mouse cortical cultures. Brain Res 2007, 169, 129-136.10.1016/j.brainres.2007.06.04717662261 Search in Google Scholar

20. Pandey G., Pandey S.P., Sharma M.: Experimental hepatotoxicity produced by ethinyl estradiol. Toxicol Int 2011, 18, 160-162.10.4103/0971-6580.84270318362521976823 Search in Google Scholar

21. Pohjala L., Tammela P., Samanta S.K., Yli-Kauhaluoma J., Vuorela P.: Assessing the data quality in predictive toxicology using a panel of cell lines and cytotoxicity assays. Anal Biochem 2007, 362, 221-228.10.1016/j.ab.2006.12.03817266913 Search in Google Scholar

22. Radošević K., Tonković T., Slivac I., Kniewald Z., Gaurina Srček V.: Comparison of cytotoxicity induced by 17α- ethynylestradiol and diethylstilbestrol in fish CCO and mammalian CHO-K1 cell lines. Bull Environ Contam Toxicol 2011, 86, 252-257.10.1007/s00128-011-0217-z21327609 Search in Google Scholar

23. Robertson C.N., Robertson K.M., Padilla G.M.:, O`Brien E.T., Cook J.M., Kim C.S., Fine R.L.: Induction of apoptosis by diethylstilbestrol in hormone insensitive prostate cancer cells. J Natl Cancer Inst 1996, 88, 908-917.10.1093/jnci/88.13.9088656443 Search in Google Scholar

24. Schoonen W.G.E.J., Westerink W.M.A., de Roos J.A.D.M., Débiton E.: Cytotoxic effects of 100 reference compounds on HepG2 and HeLa cells and of 60 compounds on ECC-1 and CHO cells. I Mechanistic assays on ROS, glutathione depletion and calcein uptake. Toxicol in Vitro 2005, 19, 505-516.10.1016/j.tiv.2005.01.00315826808 Search in Google Scholar

25. Thorpe K.L., Cummings R.I., Hutchinson T.H., Scolze M., Brighty G., Sumptem J.P.: Relative potencies and combination effects of steroidal estrogens in fish. Environ Sci Technol 2003, 37, 1142-1149.10.1021/es020134812680667 Search in Google Scholar

26. van Amsterdam J., Opperhuizen A., Hartgens F.: Adverse health effects of anabolic-androgenic steroids. Regul Toxicol Pharmacol 2010, 57, 117-123.10.1016/j.yrtph.2010.02.00120153798 Search in Google Scholar

27. Wan L., O`Brien P.: Molecular mechanism of 17α- ethinylestradiol cytotoxicity in isolated rat hepatocytes. Can J Physiol Pharmacol 2014, 92, 21-26.10.1139/cjpp-2013-026724383869 Search in Google Scholar

28. Welder A.A., Robertson J.W., Fugate R.D., Melchert R.B.: Anabolic-androgenic steroid induced toxicity in primary neonatal rat myocardial cell cultures. Toxicol Appl Pharmacol 1995, 33, 328-342.10.1006/taap.1995.11587645030 Search in Google Scholar

29. Woźniak B.: Steroid hormones - properties, application, residues in food of animal origin. Med Weter 2010, 66, 177-181. Search in Google Scholar

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Life Sciences, Molecular Biology, Microbiology and Virology, other, Medicine, Veterinary Medicine