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<p> The aim of the study was to investigate if the enterotoxigenic strains of S. aureus isolated from raw milk are able to produce staphylococcal enterotoxins (SEs) A - E. A total of 168 of S. aureus isolates from raw milk collected in the south - east region of Poland (Lubelskie Province) were tested for SE production by the ELFA, while multiplex PCR was applied for detection of enterotoxin genes (sea, seb, sec, sed, see). It was found that 20 (11.9%) out of 168 strains were positive for one or more classical SE markers and 19 of them produced a detectable level of enterotoxins. The results obtained by mPCR and ELFA were in agreement, when the presence of A, B, and D toxin types was tested; whereas SEC was not found by the ELFA although the S. aureus was positive for the respective gene. The results of the two methods showed that mPCR identified one more strain potentially producing enterotoxin than the ELFA, which may suggest that the enterotoxigenic S. aureus are not always able to express the toxin protein.</p>
</abstract>

The aim of the study was to investigate if the enterotoxigenic strains of S. aureus isolated from raw milk are able to produce staphylococcal enterotoxins (SEs) A - E. A total of 168 of S. aureus isolates from raw milk collected in the south - east region of Poland (Lubelskie Province) were tested for SE production by the ELFA, while multiplex PCR was applied for detection of enterotoxin genes (sea, seb, sec, sed, see). It was found that 20 (11.9%) out of 168 strains were positive for one or more classical SE markers and 19 of them produced a detectable level of enterotoxins. The results obtained by mPCR and ELFA were in agreement, when the presence of A, B, and D toxin types was tested; whereas SEC was not found by the ELFA although the S. aureus was positive for the respective gene. The results of the two methods showed that mPCR identified one more strain potentially producing enterotoxin than the ELFA, which may suggest that the enterotoxigenic S. aureus are not always able to express the toxin protein.

Detection of classical genes and enterotoxins of Staphylococcus aureus isolated from raw milk in the south-east region of Poland

Department of Hygiene of Food of Animal Origin

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The aim of the study was to investigate if the enterotoxigenic strains of S. aureus isolated from raw milk are able to produce staphylococcal enterotoxins...

Detection of classical genes and enterotoxins of Staphylococcus aureus isolated from raw milk in the south-east region of Poland

Published Online: Dec 20, 2014

Page range: 559 - 561

Received: Jun 12, 2014

Accepted: Oct 16, 2014

DOI: https://doi.org/10.2478/bvip-2014-0086

Keywords
raw milk, S. aureus, staphylococcal enterotoxins, ELFA, PCR, Poland

© 2014 National Veterinary Research Institute in Pulawy

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Detection of classical genes and enterotoxins of Staphylococcus aureus isolated from raw milk in the south-east region of Poland

Published Online: Dec 20, 2014

Page range: 559 - 561

Received: Jun 12, 2014

Accepted: Oct 16, 2014

DOI: https://doi.org/10.2478/bvip-2014-0086

Keywordsraw milk, S. aureus, staphylococcal enterotoxins, ELFA, PCR, Poland

© 2014 National Veterinary Research Institute in Pulawy

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
raw milk, S. aureus, staphylococcal enterotoxins, ELFA, PCR, Poland