<abstract xmlns="http://www.w3.org/1999/xhtml">
<p>The expression of miRNA-21, miRNA-10b, and miRNA-34a in malignant and benign tumours and non-neoplastic lesions in canine mammary gland, using real-time PCR with TaqMan probes was determined. The expression in normal tissues was compared to neoplastic and non-neoplastic lesions using one-way ANOVA test. Significant changes in miRNA expression in neoplastic tissues, as compared to normal ones, were demonstrated. In all neoplastic tissues, the miRNA-21 expression increased while in non-neoplastic lesions slightly decreased in comparison to normal ones. MiRNA-10b expression in malignant and benign tumours increased in comparison to normal tissues and non-neoplastic lesions. MiRNA-34a expression profile in neoplastic and non-neoplastic tissues differed from other examined miRNAs (miRNA-21 and miRNA-10b). In all samples miRNA-34a expression level decreased in comparison to normal tissues.</p>
</abstract>

The expression of miRNA-21, miRNA-10b, and miRNA-34a in malignant and benign tumours and non-neoplastic lesions in canine mammary gland, using real-time PCR with TaqMan probes was determined. The expression in normal tissues was compared to neoplastic and non-neoplastic lesions using one-way ANOVA test. Significant changes in miRNA expression in neoplastic tissues, as compared to normal ones, were demonstrated. In all neoplastic tissues, the miRNA-21 expression increased while in non-neoplastic lesions slightly decreased in comparison to normal ones. MiRNA-10b expression in malignant and benign tumours increased in comparison to normal tissues and non-neoplastic lesions. MiRNA-34a expression profile in neoplastic and non-neoplastic tissues differed from other examined miRNAs (miRNA-21 and miRNA-10b). In all samples miRNA-34a expression level decreased in comparison to normal tissues.

MiRNA-21, miRNA-10b, and miRNA-34a Expression in Canine Mammary Gland Neoplasms

Department of Pathophysiology, Forensic Veterinary and Administration

Department of Clinical Sciences, Faculty of Veterinary Medicine

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"MiRNA-21, miRNA-10b, and miRNA-34a Expression in Canine Mammary Gland Neoplasms"}

The expression of miRNA-21, miRNA-10b, and miRNA-34a in malignant and benign tumours and non-neoplastic lesions in canine mammary gland, using real-time PCR...

MiRNA-21, miRNA-10b, and miRNA-34a Expression in Canine Mammary Gland Neoplasms

Published Online: Oct 01, 2014

Page range: 447 - 451

Received: Apr 07, 2014

Accepted: Aug 28, 2014

DOI: https://doi.org/10.2478/bvip-2014-0068

Keywords
dog, mammary gland tumours, miRNA, real-time PCR

© 2014 National Veterinary Research Institute in Pulawy

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

MiRNA-21, miRNA-10b, and miRNA-34a Expression in Canine Mammary Gland Neoplasms

Published Online: Oct 01, 2014

Page range: 447 - 451

Received: Apr 07, 2014

Accepted: Aug 28, 2014

DOI: https://doi.org/10.2478/bvip-2014-0068

Keywordsdog, mammary gland tumours, miRNA, real-time PCR

© 2014 National Veterinary Research Institute in Pulawy

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
dog, mammary gland tumours, miRNA, real-time PCR