NodC -based evaluation of the occurrence of bacteria in nodules of Pisum sativum isolated on YEM agar
, , and | Jun 11, 2019
About this article
Article Category: Research Article
Published Online: Jun 11, 2019
Page range: 59 - 67
Received: Oct 03, 2018
Accepted: Dec 10, 2018
DOI: https://doi.org/10.2478/boku-2019-0005
Keywords
© 2019 Anna Lenart-Boroń, Tadeusz Zając, Piotr Mateusz Boroń, Agnieszka Klimek-Kopyra, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Figure 1
Detection of nodC gene in the studied bacterial isolatesTabelle 4. Nachweis des nodC-Gens in den untersuchten Bakterienisolaten
| Isolate No. | Variant | Nodule | Number of isolates per variant | |
|---|---|---|---|---|
| 1 | Klif (uninoculated) | Large red | 1 | Yes |
| 2 | Klif (uninoculated) | Large red | 2 | No |
| 3 | Klif (uninoculated) | Small red | 1 | No |
| 4 | Klif (uninoculated) | Small red | 2 | No |
| 5 | Klif (uninoculated) | Large cream | 1 | Yes |
| 6 | Klif (uninoculated) | Small cream | 1 | Yes |
| 7 | Klif (uninoculated) | Small cream | 2 | No |
| 8 | Tarchalska (uninoculated) | Large red | 1 | Yes |
| 9 | Tarchalska (uninoculated) | Small red | 1 | Yes |
| 10 | Tarchalska (uninoculated) | Small red | 2 | No |
| 11 | Tarchalska (uninoculated) | Large cream | 1 | Yes |
| 12 | Tarchalska (uninoculated) | Small cream | 2 | Yes |
| 13 | Klif + IUNG | Large red | 1 | Yes |
| 14 | Klif + IUNG | Small red | 1 | Yes |
| 15 | Klif + IUNG | Large cream | 1 | Yes |
| 16 | Klif + IUNG | Small cream | 1 | No |
| 17 | Tarchalska + IUNG | Large red | 1 | Yes |
| 18 | Tarchalska + IUNG | Large red | 2 | No |
| 19 | Tarchalska + IUNG | Large red | 3 | No |
| 20 | Tarchalska + IUNG | Large red | 4 | No |
| 21 | Tarchalska + IUNG | Small red | 1 | No |
| 22 | Tarchalska + IUNG | Small red | 2 | No |
| 23 | Tarchalska + IUNG | Small red | 3 | No |
| 24 | Tarchalska/IUNG | Large cream | 1 | No |
| 25 | Tarchalska + IUNG | Large cream | 2 | No |
| 26 | Tarchalska + IUNG | Large cream | 3 | No |
| 27 | Tarchalska + IUNG | Small cream | 1 | No |
| 28 | Klif + Nitragine™ | Large red | 1 | No |
| 29 | Klif + Nitragine™ | Large red | 2 | No |
| 30 | Klif + Nitragine™ | Large red | 3 | No |
| 31 | Klif + Nitragine™ | Large red | 4 | No |
| 32 | Klif + Nitragine™ | Small red | 1 | Yes |
| 33 | Klif + Nitragine™ | Small red | 2 | No |
| 34 | Klif + Nitragine™ | Large cream | 1 | Yes |
| 35 | Klif + Nitragine™ | Small cream | 1 | Yes |
| 36 | Tarchalska/Nitragine™ | Large red | 1 | Yes |
| 37 | Tarchalska + Nitragine™ | Large red | 2 | No |
| 38 | Tarchalska + Nitragine™ | Small red | 1 | Yes |
| 39 | Tarchalska + Nitragine™ | Large cream | 1 | Yes |
| 40 | Tarchalska + Nitragine™ | Small cream | 1 | Yes |
| 41 | Tarchalska + Nitragine™ | Small cream | 2 | Yes |
| 42 | Tarchalska/Nitragine™ | Small cream | 3 | Yes |
| 43 | Tarchalska + Nitragine™ | Small cream | 4 | Yes |
| 44 | Nitragine™ | Nonapplicable | 1 | No |
| 45 | IUNG | Nonapplicable | 1 | Yes |
| 46 | Nonapplicable | 1 | No |
Oligonucleotides used for nodC amplificationTabelle 1. Für die nodC-Amplifikation verwendete Oligonukleotide
| Primer | 5′–3′ nucleotide sequence |
|---|---|
| nodCF | AYGTHGTYGAYGACGGTTC |
| nodCFu | AYGTHGTYGAYGACGGITC |
| nodCF2 | AYGTHGTYGAYGACGGCTC |
| nodCF4 | AYGTHGTYGAYGACGGATC |
| nodCFn | AGGTGGTYGAYGACGGTTC |
| nodCI | CGYGACAGCCANTCKCTATTG |
Identification of bacteria colonizing pea nodulesTabelle 3. Identifizierung der Bakterien, welche die Erbsenknöllchen besiedelten
| Variant | Bacteria species | Maximum identity (%) |
|---|---|---|
| IUNG | 99 | |
| NitragineTM | 99 | |
| Klif (uninoculated) | 99 | |
| Tarchalska (uninoculated) | 99 | |
| Tarchalska + IUNG | 99 | |
| Klif + NitragineTM | 98 | |
| Tarchalska + NitragineTM | 99 |
The mean numbers of colony forming units (CFUs) of bacteria colonizing pea nodules per one nodule depending on variants (pea varieties and inoculants).Tabelle 2. Die Durchschnittswerte koloniebildender Einheiten (KBE) von Bakterien, die Erbsenknollen besiedeln, pro Knöllchen in Abhängigkeit der Varianten (Erbsensorten und Inokulen).
| Variant | Nodules | ||||
|---|---|---|---|---|---|
| Large cream | Small cream | Large red | Small red | Mean | |
| Klif (uninoculated) | 20.5 | 87.0 | 128.5 | 52.0 | |
| Tarchalska (uninoculated) | 6.0 | 110.0 | 157.5 | 36.0 | |
| Klif + IUNG | 84.0 | 97.5 | 310.0 | 212.0 | |
| Tarchalska + IUNG | 110.0 | 45.0 | 101.0 | 49.0 | |
| Klif + NitragineTM | 54.0 | 79.5 | 67.0 | 39.0 | |
| Tarchalska + NitragineTM | 45.0 | 54.0 | 32.0 | 53.0 | |