<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Chronic lymphocytic leukemia (CLL) is a neoplasm characterized by excessive accumulation of B lymphocytes in the peripheral blood, bone marrow and lymph nodes. We assessed the expressions of 22 genes in the p53 pathway in 30 CLL patients and 15 healthy subjects by a RT2 Profiler PCR (polymerase chain reaction) Array technique and their relation to cytogenetic aberrations detected by fluorescent <italic>in situ</italic> hybridization (FISH). Our Student’s <italic>t</italic>-test results indicated that <italic>ATM, ATR, BAX, CASP9, CDK4, CDKN2A, CHEK1, CHEK2, E2F3, MCL1, MDM2, MDM4, PCNA, RB1, P53</italic> and BCL2 genes were statistically significant (<italic>p</italic> &lt;0.001). For six genes <italic>(APAF1, CDKN1A, E2F1, GADD45A, PTEN</italic> and <italic>PTX3)</italic> were not statistically significant. The <italic>ATM, ATR, BAX, CASP9, CDK4, CDKN1A, CDKN2A, CHEK1, CHEK2, MDM2, MDM4, PCNA, RB1, P53, E2F1, GADD45A</italic> and <italic>BCL2</italic> genes were found to be upregulated by the 2<sup>-ᐃᐃCt</sup> (relative fold change in gene expression) method. The highest up-regulation was detected in <italic>CDKN2A</italic> and <italic>BCL2</italic> genes, 10.22- and 8.51-fold, respectively. On the other hand, the <italic>PTX3</italic> gene with a fold regulation of 1.84 was found to the highest downregulation. Overall, the <italic>CDNK2A BCL2</italic> and <italic>PTX3</italic> genes are related to the mechanism of the disease in the p53 pathway and may be an important predictor of the prognosis of the disease. The <italic>BCL2</italic> gene may be associated with increased risk of developing CLL. We suggest that the <italic>PTX3</italic> gene may be considered as a marker associated with CLL disease. The <italic>CDKN2A</italic> gene expression seems to play a protective role in CLL.</p></abstract>

Chronic lymphocytic leukemia (CLL) is a neoplasm characterized by excessive accumulation of B lymphocytes in the peripheral blood, bone marrow and lymph nodes. We assessed the expressions of 22 genes in the p53 pathway in 30 CLL patients and 15 healthy subjects by a RT2 Profiler PCR (polymerase chain reaction) Array technique and their relation to cytogenetic aberrations detected by fluorescent in situ hybridization (FISH). Our Student’s t-test results indicated that ATM, ATR, BAX, CASP9, CDK4, CDKN2A, CHEK1, CHEK2, E2F3, MCL1, MDM2, MDM4, PCNA, RB1, P53 and BCL2 genes were statistically significant (p <0.001). For six genes (APAF1, CDKN1A, E2F1, GADD45A, PTEN and PTX3) were not statistically significant. The ATM, ATR, BAX, CASP9, CDK4, CDKN1A, CDKN2A, CHEK1, CHEK2, MDM2, MDM4, PCNA, RB1, P53, E2F1, GADD45A and BCL2 genes were found to be upregulated by the 2-ᐃᐃCt (relative fold change in gene expression) method. The highest up-regulation was detected in CDKN2A and BCL2 genes, 10.22- and 8.51-fold, respectively. On the other hand, the PTX3 gene with a fold regulation of 1.84 was found to the highest downregulation. Overall, the CDNK2A BCL2 and PTX3 genes are related to the mechanism of the disease in the p53 pathway and may be an important predictor of the prognosis of the disease. The BCL2 gene may be associated with increased risk of developing CLL. We suggest that the PTX3 gene may be considered as a marker associated with CLL disease. The CDKN2A gene expression seems to play a protective role in CLL.

Relationship between chromosomal aberrations and gene expressions in the p53 pathway in chronic lymphocytic leukemia

Balkan Journal of Medical Genetics

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Chronic lymphocytic leukemia (CLL) is a neoplasm characterized by excessive accumulation of B lymphocytes in the peripheral blood, bone marrow and lymph nodes....

Relationship between chromosomal aberrations and gene expressions in the p53 pathway in chronic lymphocytic leukemia

Article Category: Original Article

Published Online: Aug 26, 2020

Page range: 15 - 24

DOI: https://doi.org/10.2478/bjmg-2020-0007

Keywords
Chronic lymphocytic leukemia (CLL), gene expression, p53 Pathway, Prognosis, RT Profiler PCR (polymerase chain reaction)Array

© 2020 Öztan G, Aktan M, Palanduz S, İşsever H, Öztürk S, Nikerel E, Uçur A, Bağatir G, Bayrak B, Çefle K, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Relationship between chromosomal aberrations and gene expressions in the p53 pathway in chronic lymphocytic leukemia

Article Category: Original Article

Published Online: Aug 26, 2020

Page range: 15 - 24

DOI: https://doi.org/10.2478/bjmg-2020-0007

KeywordsChronic lymphocytic leukemia (CLL), gene expression, p53 Pathway, Prognosis, RT Profiler PCR (polymerase chain reaction)Array

© 2020 Öztan G, Aktan M, Palanduz S, İşsever H, Öztürk S, Nikerel E, Uçur A, Bağatir G, Bayrak B, Çefle K, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
Chronic lymphocytic leukemia (CLL), gene expression, p53 Pathway, Prognosis, RT Profiler PCR (polymerase chain reaction)Array