Determining specific thyroid transcripts in peripheral blood: A single center study experience

T Makazlieva; A Eftimov; O Vaskova; T Tripunoski; D Miladinova; S Risteski; H Jovanovic; Z Jakovski

Open Access

Determining specific thyroid transcripts in peripheral blood: A single center study experience

and

| Dec 31, 2018

Balkan Journal of Medical Genetics

Volume 21 (2018): Issue 2 (December 2018)

About this article

Cite

Published Online: Dec 31, 2018

Page range: 13 - 20

DOI: https://doi.org/10.2478/bjmg-2018-0015

Keywords
Thyroglobulin () gene expression, Thyroid carcinoma (TC), Thyroid stimulating hormone receptor () gene expression

© 2018 Makazlieva T, Eftimov A, Vaskova O, Tripunoski T, Miladinova D, Risteski S, Jovanovic H, Jakovski Z, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Distribution of ΔCt = (CtTSHR-CtGAPDH) among four evaluated groups (see tables).

Two-group comparison for ΔCt = (CtTSHR-CtGAPDH) values (see tables).

Four-group comparison for ΔCt = (CtTg-CtGAPDH) values (see tables).

Two-group comparison of ΔCt = (CtTg-CtGAPDH) (see tables).

Intergroup paired comparison in ΔCt = (CtTg-CtGAPDH) values.

Mann-Whitney U Test	TCs^a/TCb^b	TCs^a/TCr^c	TCs^a/HC^d	TCb^b/TCr^c	TCb^b/HC^d	TCr^c/HC^d
Z value	–1.166	–1.964	–2.453	–0.402	–0.442	0.148
Asymptotic significance (two-tailed)	0.243	0.049^e	0.014^e	0.688	0.658	0.882

Fold change in expression of TSHR and Tg target genes between TCs and HC/TCr groups and between TCb and HC/TCr groups.

Fold Change	For TSHR-mRNA	For Tg-mRNA
ΔΔCt = ΔCt (TCs)-ΔCt (HC) 2^–ΔΔCt	–3.09904 (8.57)	–2.72137 (6.6)
ΔΔCt = ΔCt (TCs)-ΔCt (TCr) 2^–ΔΔCt	–2.42523 (5.37)	–3.06057 (8.34)
ΔΔCt = ΔCt (TCb)-ΔCt (HC) 2^–ΔΔCt	–3.82478 (14.17)	–0.61184 (1.53)
ΔΔCt = ΔCt (TCb)-ΔCt (TCr) 2^–ΔΔCt	–3.15097 (8.88)	–0.95104 (1.93)

Intergroup paired comparison in ΔCt = (CtTSHR-CtGAPDH) values.

Mann-Whitney U Test	TCs^a/TCb^b	TCs^a/TCr^c	TCs^a/HC^d	TCb^b/TCr^c	TCb^b/HC^d	TCr^c/HC^d
Z value	0.382	–2.231	–3.416	–2.341	–2.803	–1.757
Asymptotic significance (two-tailed)	0.703	0.026^e	0.0006^e	0.019^e	0.005^e	0.079

Two-group comparison for Ct = (CtTg-CtGAPDH) values.

Groups	Mean	n	SD	Minimum	Maximum		Percentiles
						25^th	50^th (median)	75^th
TCs^a+TCb^b	6.81	27	4.91	–5.49	15.00	4.915	8.35	10.17
TCr^c+HD^d	9.82	27	2.53	5.71	15.45	8.14	9.62	10.85
Summary	8.32	54	4.16	–5.49	15.45	7.23	8.86	10.37

Four intergroup comparisons for ΔCt = (CtTSHR-CtGAPDH) values.

Groups	Mean	n	SD	Minimum	Maximum	Percentiles
						25^th	50^th (median)	75^th
TCs^a	9.62	19	2.66	4.66	13.13	7.47	9.58	12.26
TCb^b	8.89	6	3.26	4.15	12.55	5.63	10.13	10.77
TCr^c	12.04	12	1.76	10.59	17.21	11.06	11.55	12.29
HC^d	12.72	15	1.65	10.36	17.00	11.39	12.66	13.57
Summary	10.99	52	2.70	4.15	17.21	10.13	11.21	12.57

Four-group comparison for ΔCt = (CtTg-CtGAPDH) values.

Groups	Mean	n	SD	Minimum	Maximum	Percentiles
						25^th	50^th (median)	75^th
TCs^a	6.17	21	5.37	–5.49	15.00	2.74	7.79	9.40
TCb^b	9.07	6	1.55	7.22	10.61	7.39	9.36	10.47
TCr^c	10.02	11	2.60	6.01	15.45	7.82	9.62	12.17
HC^d	9.68	16	2.28	5.71	13.85	8.81	9.69	10.64
Summary	8.32	54	4.16	5.49	15.45	7.23	8.86	10.37

Two-group comparison for ΔCt = (TSHR-GAPDH) values.

Groups	Mean	n	SD	Minimum	Maximum	Percentiles
						25^th	50^th (median)	75^th
TCs^a+TCb^b	9.44	25	2.76	4.15	13.13	7.465	9.94	11.66
TCr^c+HC^d	12.42	27	1.70	10.36	17.21	11.20	12.05	13.33
Summary	10.99	52	2.70	4.15	17.21	10.12	11.21	12.57

eISSN:: 1311-0160
Language:: English

Publication timeframe:: 2 times per year
Journal Subjects:: Medicine, Basic Medical Science, other

Journal RSS Feed

Determining specific thyroid transcripts in peripheral blood: A single center study experience

Article Category: Original Article

Published Online: Dec 31, 2018

Page range: 13 - 20

DOI: https://doi.org/10.2478/bjmg-2018-0015

Keywords
Thyroglobulin () gene expression, Thyroid carcinoma (TC), Thyroid stimulating hormone receptor () gene expression

© 2018 Makazlieva T, Eftimov A, Vaskova O, Tripunoski T, Miladinova D, Risteski S, Jovanovic H, Jakovski Z, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1

Figure 2

Figure 3

Figure 4

Intergroup paired comparison in ΔCt = (CtTg-CtGAPDH) values.

Fold change in expression of TSHR and Tg target genes between TCs and HC/TCr groups and between TCb and HC/TCr groups.

Intergroup paired comparison in ΔCt = (CtTSHR-CtGAPDH) values.

Two-group comparison for Ct = (CtTg-CtGAPDH) values.

Four intergroup comparisons for ΔCt = (CtTSHR-CtGAPDH) values.

Four-group comparison for ΔCt = (CtTg-CtGAPDH) values.

Two-group comparison for ΔCt = (TSHR-GAPDH) values.

Determining specific thyroid transcripts in peripheral blood: A single center study experience

Article Category: Original Article

Published Online: Dec 31, 2018

Page range: 13 - 20

DOI: https://doi.org/10.2478/bjmg-2018-0015

KeywordsThyroglobulin () gene expression, Thyroid carcinoma (TC), Thyroid stimulating hormone receptor () gene expression

© 2018 Makazlieva T, Eftimov A, Vaskova O, Tripunoski T, Miladinova D, Risteski S, Jovanovic H, Jakovski Z, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1

Figure 2

Figure 3

Figure 4

Intergroup paired comparison in ΔCt = (CtTg-CtGAPDH) values.

Fold change in expression of TSHR and Tg target genes between TCs and HC/TCr groups and between TCb and HC/TCr groups.

Intergroup paired comparison in ΔCt = (CtTSHR-CtGAPDH) values.

Two-group comparison for Ct = (CtTg-CtGAPDH) values.

Four intergroup comparisons for ΔCt = (CtTSHR-CtGAPDH) values.

Four-group comparison for ΔCt = (CtTg-CtGAPDH) values.

Two-group comparison for ΔCt = (TSHR-GAPDH) values.

Keywords
Thyroglobulin () gene expression, Thyroid carcinoma (TC), Thyroid stimulating hormone receptor () gene expression