<abstract xmlns="http://www.w3.org/1999/xhtml"><p> The ageing process is influenced by many internal and external factors. The toxic substances in the environment can cause genomic damages to cells, which increase the risk of early ageing. Furthermore, the cytochrome P450 1A2 (CYP1A2) gene polymorphism is a susceptibility factor and may enhance the risk of DNA damage in cells. The current study was carried out to show whether occupational exposure could cause genotoxicity in cells carrying the CYP1A2 gene polymorphism, thus enhancing the likelihood of early ageing. This study was conducted on mechanical workshop workers and a control group by collecting buccal cells from their mouths. Restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) was used to identify the CYP1A2 gene polymorphism in the cells. In addition, three extra methods including micronuclei (MN) test, comet assay and real-time PCR (RT-PCR) were applied to determine the effects of gene polymorphisms on DNA damage and ageing from occupational exposure. The results showed that DNA damage in the cells carrying the mutated genotype was higher than the wild genotype. In addition, the difference in MN frequency (p = 0.001) and relative telomere length (p = 0.002) between workers and controls was significant (p &lt;0.05) in the mutated genotype. The findings indicated a possible protective effect of gene polymorphism against early ageing, which was characterized by lack of a significant influence of CYP1A2 gene polymorphism on genetic material in the subjects (p >0.05). It was concluded that the CYP1A2 gene could be a contributing factor to prevent early ageing from occupational exposure.</p></abstract>

The ageing process is influenced by many internal and external factors. The toxic substances in the environment can cause genomic damages to cells, which increase the risk of early ageing. Furthermore, the cytochrome P450 1A2 (CYP1A2) gene polymorphism is a susceptibility factor and may enhance the risk of DNA damage in cells. The current study was carried out to show whether occupational exposure could cause genotoxicity in cells carrying the CYP1A2 gene polymorphism, thus enhancing the likelihood of early ageing. This study was conducted on mechanical workshop workers and a control group by collecting buccal cells from their mouths. Restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) was used to identify the CYP1A2 gene polymorphism in the cells. In addition, three extra methods including micronuclei (MN) test, comet assay and real-time PCR (RT-PCR) were applied to determine the effects of gene polymorphisms on DNA damage and ageing from occupational exposure. The results showed that DNA damage in the cells carrying the mutated genotype was higher than the wild genotype. In addition, the difference in MN frequency (p = 0.001) and relative telomere length (p = 0.002) between workers and controls was significant (p <0.05) in the mutated genotype. The findings indicated a possible protective effect of gene polymorphism against early ageing, which was characterized by lack of a significant influence of CYP1A2 gene polymorphism on genetic material in the subjects (p >0.05). It was concluded that the CYP1A2 gene could be a contributing factor to prevent early ageing from occupational exposure.

ROLE OF THE CYP1A2 GENE POLYMORPHISM ON EARLY AGEING FROM OCCUPATIONAL EXPOSURE

Institute of Gerontology, Universiti Putra Malaysia (UPM), Serdang, Selangor, Malaysia Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia (UPM), Serdang, Selangor, Malaysia

Institute of Gerontology, Universiti Putra Malaysia (UPM), Serdang, Selangor, Malaysia

Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia (UPM), Serdang, Selangor, Malaysia

Institute for Medical Research, Ministry of Health Malaysia, Kuala Lumpur, Malaysia

Balkan Journal of Medical Genetics

{"article-title":"ROLE OF THE CYP1A2 GENE POLYMORPHISM ON EARLY AGEING FROM OCCUPATIONAL EXPOSURE"}

The ageing process is influenced by many internal and external factors. The toxic substances in the environment can cause genomic damages to cells, which increase the risk of early ageing. Furthermore, the cytochrome P450 1A2 (CYP1A2) gene polymorphism is a susceptibility factor and may enhance the risk of DNA damage in cells. The current study was carried out to show whether occupational exposure could cause genotoxicity in cells carrying the CYP1A2 gene polymorphism, thus enhancing the likelihood of early ageing. This study was conducted on mechanical workshop workers and a control group by collecting buccal cells from their mouths. Restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) was used to identify the CYP1A2 gene polymorphism in the cells. In addition, three extra methods including micronuclei (MN) test, comet assay and real-time PCR (RT-PCR) were applied to determine the effects of gene polymorphisms on DNA damage and ageing from occupational exposure. The results showed that DNA damage in the cells carrying the mutated genotype was higher than the wild genotype. In addition, the difference in MN frequency (p = 0.001) and relative telomere length (p = 0.002) between workers and controls was significant (p 0.05). It was concluded that the CYP1A2 gene could be a contributing factor to prevent early ageing from occupational exposure.

The ageing process is influenced by many internal and external factors. The toxic substances in the environment can cause genomic damages to cells, which...

ROLE OF THE CYP1A2 GENE POLYMORPHISM ON EARLY AGEING FROM OCCUPATIONAL EXPOSURE

Published Online: Mar 11, 2014

Page range: 45 - 52

DOI: https://doi.org/10.2478/bjmg-2013-0031

Keywords
Ageing, Buccal Cells, Cytochrome P450 (CYP), Cytochrome P450 1A2 (CYP1A2) gene polymorphism, Micronuclei (MN) test, Occupational exposure

This content is open access.

ROLE OF THE CYP1A2 GENE POLYMORPHISM ON EARLY AGEING FROM OCCUPATIONAL EXPOSURE

Published Online: Mar 11, 2014

Page range: 45 - 52

DOI: https://doi.org/10.2478/bjmg-2013-0031

KeywordsAgeing, Buccal Cells, Cytochrome P450 (CYP), Cytochrome P450 1A2 (CYP1A2) gene polymorphism, Micronuclei (MN) test, Occupational exposure

This content is open access.

Keywords
Ageing, Buccal Cells, Cytochrome P450 (CYP), Cytochrome P450 1A2 (CYP1A2) gene polymorphism, Micronuclei (MN) test, Occupational exposure