<abstract xmlns="http://www.w3.org/1999/xhtml"><p>This research was performed to ascertain the ubiquity of bacterial pathogens which hyper-produced AmpC <italic>β</italic>-lactamase enzymes in adult catfish cultured in the Nigerian catfish grow-out pond systems. Phenotypic and molecular methods were used to isolate and identify bacterial pathogens that hyper-produced AmpC <italic>β</italic>-lactamase enzymes. The AmpC <italic>β</italic>-lactamase enzymes produced by the bacterial pathogens were subsequently characterized by BLASTX and RPS-BLAST bioinformatics software as well as with the Cn3D molecular modelling software. Findings from the present study indicated that pathogenic bacterial strains which hyper-produced the AmpC <italic>β</italic>-lactamase enzymes were isolated from 49 catfish samples out of the 54 catfish samples which were examined. The pathogenic bacterial strains were mainly identified as <italic>Citrobacter freundii</italic> MGH 150, <italic>Enterobacter cloacae</italic> NG 14, and <italic>Enterobacter cloacae</italic> subspecies <italic>dissolvens</italic> HKE 15. The AmpC <italic>β</italic>-lactamase enzymes produced by the bacterial pathogens were also respectively identified as cephalosporinase hydrolyzing class C CMY-LAT-MOX-ACT-MIR-FOX, CMY2/MIR/ACT/EC family class C beta-lactamase, and CMY2/MIR/ACT/EC family class C beta-lactamase. The presence of AmpC enzymes that are hyper-produced by bacterial pathogens which were isolated from almost all the catfish examined calls for urgent monitoring/surveillance of the Nigerian catfish ponds by the relevant regulatory agencies.</p></abstract>

This research was performed to ascertain the ubiquity of bacterial pathogens which hyper-produced AmpC β-lactamase enzymes in adult catfish cultured in the Nigerian catfish grow-out pond systems. Phenotypic and molecular methods were used to isolate and identify bacterial pathogens that hyper-produced AmpC β-lactamase enzymes. The AmpC β-lactamase enzymes produced by the bacterial pathogens were subsequently characterized by BLASTX and RPS-BLAST bioinformatics software as well as with the Cn3D molecular modelling software. Findings from the present study indicated that pathogenic bacterial strains which hyper-produced the AmpC β-lactamase enzymes were isolated from 49 catfish samples out of the 54 catfish samples which were examined. The pathogenic bacterial strains were mainly identified as Citrobacter freundii MGH 150, Enterobacter cloacae NG 14, and Enterobacter cloacae subspecies dissolvens HKE 15. The AmpC β-lactamase enzymes produced by the bacterial pathogens were also respectively identified as cephalosporinase hydrolyzing class C CMY-LAT-MOX-ACT-MIR-FOX, CMY2/MIR/ACT/EC family class C beta-lactamase, and CMY2/MIR/ACT/EC family class C beta-lactamase. The presence of AmpC enzymes that are hyper-produced by bacterial pathogens which were isolated from almost all the catfish examined calls for urgent monitoring/surveillance of the Nigerian catfish ponds by the relevant regulatory agencies.

AmpC beta-lactamase enzymes are ubiquitous in catfish (<italic>Clarias gariepinus</italic>) cultured in the Nigerian catfish grow-out pond systems

Department of Microbiology, Faculty of Life Sciences

Ovidius University Annals of Chemistry

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

AmpC beta-lactamase enzymes are ubiquitous in catfish () cultured in the Nigerian catfish grow-out pond systems

This research was performed to ascertain the ubiquity of bacterial pathogens which hyper-produced AmpC β-lactamase enzymes in adult catfish cultured in the...

AmpC beta-lactamase enzymes are ubiquitous in catfish (Clarias gariepinus) cultured in the Nigerian catfish grow-out pond systems

Published Online: Apr 15, 2019

Page range: 25 - 28

Received: Dec 18, 2018

Accepted: Mar 07, 2019

DOI: https://doi.org/10.2478/auoc-2019-0005

Keywords
cephalosporinase hydrolyzing class C CMY-LAT-MOX-ACT-MIR-FOX, CMY2/MIR/ACT/EC family class C beta-lactamase,

© 2019 Faith I. Akinnibosun et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

AmpC beta-lactamase enzymes are ubiquitous in catfish (Clarias gariepinus) cultured in the Nigerian catfish grow-out pond systems

Published Online: Apr 15, 2019

Page range: 25 - 28

Received: Dec 18, 2018

Accepted: Mar 07, 2019

DOI: https://doi.org/10.2478/auoc-2019-0005

Keywordscephalosporinase hydrolyzing class C CMY-LAT-MOX-ACT-MIR-FOX, CMY2/MIR/ACT/EC family class C beta-lactamase,

© 2019 Faith I. Akinnibosun et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
cephalosporinase hydrolyzing class C CMY-LAT-MOX-ACT-MIR-FOX, CMY2/MIR/ACT/EC family class C beta-lactamase,