<abstract xmlns="http://www.w3.org/1999/xhtml"><p>In present study, the effects of carbon sources (glucose, lactose, sucrose, galactose, maltose and soluble starch) and nitrogen sources (casein peptone, whey protein, soy peptone, yeast, tryptone, beef extract and peptone) on activity of cell envelope proteinases (CEP), specific activity, protein content, OD<sub>600</sub> value and pH in MRS broth fermented by <italic>Lactobacillus plantarum</italic> LP69 were investigated by individual factor experiment. The results indicated that carbon and nitrogen sources have significant influence on the activity of CEP and specific activitys of <italic>L. plantarum</italic> LP69, glucose, maltose, casein peptone and peptone are superior to other selected carbon and nitrogen sources. The optimum concentrations of glucose, maltose, casein peptone and peptone for <italic>L. plantarum</italic> LP69 are 2%, 2%, 1% and 1%; the activity of CEP are 19.52U/mL, 21.13U/mL, 13.49U/mL and 20.61U/mL, respectively.</p></abstract>

In present study, the effects of carbon sources (glucose, lactose, sucrose, galactose, maltose and soluble starch) and nitrogen sources (casein peptone, whey protein, soy peptone, yeast, tryptone, beef extract and peptone) on activity of cell envelope proteinases (CEP), specific activity, protein content, OD600 value and pH in MRS broth fermented by Lactobacillus plantarum LP69 were investigated by individual factor experiment. The results indicated that carbon and nitrogen sources have significant influence on the activity of CEP and specific activitys of L. plantarum LP69, glucose, maltose, casein peptone and peptone are superior to other selected carbon and nitrogen sources. The optimum concentrations of glucose, maltose, casein peptone and peptone for L. plantarum LP69 are 2%, 2%, 1% and 1%; the activity of CEP are 19.52U/mL, 21.13U/mL, 13.49U/mL and 20.61U/mL, respectively.

Effects of Carbon and Nitrogen Sources on Activity of Cell Envelope Proteinase Produced by <italic>Lactobacillus plantarum</italic> LP69

School of Food and Biological Engineering

Shaanxi University of Science & Technology

Acta Universitatis Cibiniensis. Series E: Food Technology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Effects of Carbon and Nitrogen Sources on Activity of Cell Envelope Proteinase Produced by  LP69

In present study, the effects of carbon sources (glucose, lactose, sucrose, galactose, maltose and soluble starch) and nitrogen sources (casein peptone, whey...

Effects of Carbon and Nitrogen Sources on Activity of Cell Envelope Proteinase Produced by Lactobacillus plantarum LP69

Published Online: Jul 18, 2019

Page range: 11 - 18

DOI: https://doi.org/10.2478/aucft-2019-0002

Keywords
LP69, cell-envelope proteinases, carbon sources, nitrogen sources

© 2019 Fangfang Cheng et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Effects of Carbon and Nitrogen Sources on Activity of Cell Envelope Proteinase Produced by Lactobacillus plantarum LP69

Published Online: Jul 18, 2019

Page range: 11 - 18

DOI: https://doi.org/10.2478/aucft-2019-0002

KeywordsLP69, cell-envelope proteinases, carbon sources, nitrogen sources

© 2019 Fangfang Cheng et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
LP69, cell-envelope proteinases, carbon sources, nitrogen sources